Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F57 I0 R1
|
50 |
10.6 |
235668 |
93.0% |
219171 |
141.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
2,187,071 |
C→T |
100% |
R331K (AGA→AAA) |
rsbU ← |
sigma factor B regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,187,071 | 0 | C | T | 100.0%
| 30.8
/ NA
| 10 | R331K (AGA→AAA) | rsbU | sigma factor B regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (4/6); total (4/6) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. |
ATTACACTCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTCTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATAT > CP000730/2186946‑2187188
|
attACACTCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCaaaa > 2:28628/1‑141 (MQ=255)
tATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGt > 1:57667/1‑141 (MQ=255)
atatttatatttCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGAt < 2:45121/141‑1 (MQ=255)
atttCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGaa < 1:51945/141‑1 (MQ=255)
aaTTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAatat < 1:7250/141‑1 (MQ=255)
tatataCCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGTACAATATCTtgtg > 2:100371/1‑141 (MQ=255)
tatataCCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTtgtg < 1:28628/141‑1 (MQ=255)
gAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCAtatgtt < 2:54705/141‑1 (MQ=255)
aaaTAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCAtatgttt > 2:108030/1‑141 (MQ=255)
cttcttTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAatat < 1:85218/141‑1 (MQ=255)
|
ATTACACTCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTCTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATAT > CP000730/2186946‑2187188
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
