Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F15 I0 R1	137	139.7	3319070	88.4%	2934057	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	512,643	A→G	52.6%	intergenic (+711/‑150)	recR → / → rrs1	recombination protein RecR/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	512,643	0	A	G	52.6%	37.4 / inf	268	intergenic (+711/‑150)	recR/rrs1	recombination protein RecR/16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (68/59); new base G (73/68); total (141/127)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-02

Only 100 of 277 total aligned reads displayed.

GGTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAACATCATAA  >  CP000730/512504‑512783

ggTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTtaa                                                                                                                                                <  1:159435/141‑1 (MQ=255)

  tgtAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTtaata                                                                                                                                              >  1:1281962/1‑141 (MQ=255)

   gtAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTtaataa                                                                                                                                             <  2:539202/141‑1 (MQ=255)

   gaagttaagatcattagcgtcgatggtagtcgaacttacgttccgctagagtagaaagttgacaggcagttttttaatcaaaaaaaaaaaaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTtgataa                                                                                                                                             >  2:175836‑M1/114‑141 (MQ=255)

      gttaagctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAAt                                                                                                                                          <  2:492587‑M1/31‑1 (MQ=255)

       tttttACTGTTGTTACGTAGTTTAAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACAAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATg                                                                                                                                         <  1:779082/141‑1 (MQ=255)

          ttACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGtat                                                                                                                                      <  2:1107488/141‑1 (MQ=255)

          agctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGtat                                                                                                                                      <  2:381047‑M1/35‑1 (MQ=255)

          agctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggaaaaaataaAAAAAGTTATTGACTTAAATGTTGATAAAATGtat                                                                                                                                      >  2:665239‑M1/107‑141 (MQ=255)

            ctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTAtaa                                                                                                                                    >  2:99053‑M1/105‑141 (MQ=255)

             tccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaataaaaaaaaaaaAAAAAGTTATTGACTTAAATGTTGATAAAATGTAtaat                                                                                                                                   >  2:967539‑M1/104‑141 (MQ=255)

              tgttgtTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTAtaatt                                                                                                                                  <  1:805243/141‑1 (MQ=255)

                ttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTAtaattaa                                                                                                                                >  1:136952‑M1/101‑141 (MQ=255)

                ttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttgattaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTAtaattaa                                                                                                                                >  2:451585‑M1/101‑141 (MQ=255)

                   gcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTc                                                                                                                             <  2:1530645‑M1/44‑1 (MQ=255)

                     gtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCtt                                                                                                                           <  2:1421736‑M1/46‑1 (MQ=255)

                       gCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGt                                                                                                                         <  2:820649/141‑1 (MQ=255)

                       cgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGt                                                                                                                         >  2:1264256‑M1/94‑141 (MQ=255)

                           tttGAAAGCGTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAAATTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGt                                                                                                                     <  1:918231/141‑1 (MQ=255)

                              agtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAg                                                                                                                  <  2:7355‑M1/55‑1 (MQ=255)

                                aaGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGaa                                                                                                                >  2:1619795/1‑141 (MQ=255)

                                   aacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatgaacaaaataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGaaaaa                                                                                                             >  2:554925‑M1/82‑141 (MQ=255)

                                    cTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑t                                                                                                         >  2:1429568/1‑141 (MQ=255)

                                      ttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGa                                                                                                       <  2:219499‑M1/63‑1 (MQ=255)

                                                ctctagcggaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGaaaa                                                                                             <  1:1123047‑M1/73‑1 (MQ=255)

                                                   gagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTg                                                                                          >  1:1035301‑M1/66‑141 (MQ=255)

                                                     gttgAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGaa                                                                                        >  1:1149240/1‑141 (MQ=255)

                                                     gtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGaa                                                                                        >  1:120941‑M1/64‑141 (MQ=255)

                                                       tgAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATg                                                                                      >  2:1136668/1‑141 (MQ=255)

                                                        gaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGa                                                                                     >  2:696796‑M1/61‑141 (MQ=255)

                                                         aacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAAAAATGAACATTGAAAACTGAAt                                                                                       <  2:1016095‑M1/82‑1 (MQ=255)

                                                         aacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGAc                                                                                    <  1:1438838‑M1/82‑1 (MQ=255)

                                                          ggCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACa                                                                                   >  2:732070/1‑141 (MQ=255)

                                                           cgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggaaaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACaa                                                                                  >  2:1142246‑M1/58‑141 (MQ=255)

                                                             aaacaaaacaACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAata                                                                                <  1:342089/141‑1 (MQ=255)

                                                              tgccaggcaggtttgtaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAatat                                                                               >  2:612483‑M1/55‑141 (MQ=255)

                                                              aacaaaacaaCTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAatat                                                                               >  1:823284/1‑141 (MQ=255)

                                                                ccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGt                                                                             >  1:1564192‑M1/53‑141 (MQ=255)

                                                                caaaacaaCTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGGTATTGACGTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGt                                                                             <  1:325566/141‑1 (MQ=255)

                                                                aaaaacaaCTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGt                                                                             <  1:438088/140‑1 (MQ=255)

                                                                 aaaacaaCTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTc                                                                            >  2:1215511/1‑141 (MQ=255)

                                                                  aggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCa                                                                           <  1:408774‑M1/91‑1 (MQ=255)

                                                                   aacaacTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATGGGAAAAAGTTATGGACTGAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCaa                                                                          <  1:1073409/141‑1 (MQ=39)

                                                                    acaacTCAACTTAAGAAATAACTTGAATTACTAACGAGAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAAc                                                                         >  1:1628078/1‑141 (MQ=255)

                                                                    acaacTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAAc                                                                         >  1:1413301/1‑141 (MQ=255)

                                                                       gttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGtt                                                                      <  2:1478905‑M1/96‑1 (MQ=255)

                                                                       gttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGtt                                                                      >  2:1442779‑M1/46‑141 (MQ=255)

                                                                       gttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGtt                                                                      >  2:1331669‑M1/46‑141 (MQ=255)

                                                                           tttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAAtt                                                                  <  2:316291‑M1/100‑1 (MQ=255)

                                                                                  gAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCaaaaa                                                           >  1:969852/1‑141 (MQ=255)

                                                                                   aattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGCAAACTGAATGACAATATGTCAACGTTAATTCCaaaaaa                                                          >  1:879103‑M1/34‑141 (MQ=255)

                                                                                    attttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAAc                                                         <  1:1526110‑M1/109‑1 (MQ=255)

                                                                                    aaTAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAAc                                                         >  1:1027421/1‑141 (MQ=255)

                                                                                      tAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑t                                                                                                         >  2:596664/1‑91 (MQ=255)

                                                                                      tAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATGCCAAAAAACGt                                                       <  1:419558/141‑1 (MQ=255)

                                                                                         ggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAAc                                                    >  2:856261‑M1/28‑141 (MQ=255)

                                                                                         ggtgaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAAc                                                    <  2:88702‑M1/114‑1 (MQ=255)

                                                                                          gttaaaaaataaaatggacaagataaAAAAAGTTATTGACATAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAaca                                                   <  2:1064955‑M1/115‑2 (MQ=255)

                                                                                          gttaaaaaataaaatggacaagataaAAAAAGTNATTNACTTAAATGTTGATAAAATGTATAATTAATTCTTTTCGGAAAGAAAAA‑‑‑AGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAATAACCNTAACt                                                   <  1:496005‑M1/115‑1 (MQ=255)

                                                                                           ttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACta                                                  <  1:200604‑M1/116‑1 (MQ=255)

                                                                                            taaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACtat                                                 >  1:1393767‑M1/25‑141 (MQ=255)

                                                                                                 aataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGtt                                            >  1:1042762‑M1/20‑141 (MQ=255)

                                                                                                 aCTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGGATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAAAAAAAAAAAAAAAAAACGTAACTATAAGtt                                            <  2:517737/141‑1 (MQ=38)

                                                                                                  cTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTa                                           >  2:831827/1‑141 (MQ=255)

                                                                                                  cTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAAGTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTa                                           <  2:1543419/141‑1 (MQ=255)

                                                                                                  ataaaatggacaagataaAAAAAGTTATTGCCTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAACATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTa                                           <  1:1215012‑M1/123‑1 (MQ=255)

                                                                                                   tAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTc                                                                                                                        <  2:721111/66‑1 (MQ=255)

                                                                                                    aaaaaggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACa                                         >  1:854177‑M1/17‑141 (MQ=255)

                                                                                                    aaCGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACa                                         <  1:670738/141‑1 (MQ=255)

                                                                                                      aatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACaaa                                       >  1:371910‑M1/15‑141 (MQ=255)

                                                                                                        tggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACa                                     <  1:57358‑M1/129‑1 (MQ=255)

                                                                                                        tggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACa                                     >  1:7415‑M1/13‑141 (MQ=255)

                                                                                                        tggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACa                                     >  1:7355‑M1/13‑141 (MQ=255)

                                                                                                             aagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGACCATTGAAAAATGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACattatt                                >  1:414319‑M1/8‑141 (MQ=255)

                                                                                                              agataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTAttt                               >  1:462617‑M1/7‑141 (MQ=255)

                                                                                                              aattTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTAttt                               >  2:1220503/1‑141 (MQ=255)

                                                                                                              aattTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTAttt                               >  1:1639360/1‑141 (MQ=255)

                                                                                                              aattTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTAttt                               >  1:277730/1‑141 (MQ=255)

                                                                                                              aattTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTACAAAAAACGTAACTATAAGTTACAAACATTAttt                               >  1:1537284/1‑141 (MQ=255)

                                                                                                               attTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTa                              >  2:1195474/1‑141 (MQ=255)

                                                                                                                ttttAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAg                             >  2:1347699/1‑141 (MQ=255)

                                                                                                                ttttAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAg                             >  1:603238/1‑141 (MQ=255)

                                                                                                                  aaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTAGAAGTTACAAACATTATTTAGTa                           >  1:1172521‑M1/3‑141 (MQ=255)

                                                                                                                   aAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTAt                          <  2:281009‑M1/140‑1 (MQ=255)

                                                                                                                     aaaaGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTAttt                        >  2:213032/1‑141 (MQ=255)

                                                                                                                      aaaGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTa                       >  1:232077/1‑141 (MQ=255)

                                                                                                                      aaaGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTa                       >  2:1169572/1‑141 (MQ=255)

                                                                                                                      aaaGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTa                       <  1:544175/141‑1 (MQ=255)

                                                                                                                         gTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGa                    >  1:113643/1‑141 (MQ=255)

                                                                                                                          ttATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAg                   <  2:1305822/141‑1 (MQ=255)

                                                                                                                          ttATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAg                   <  1:1474694/141‑1 (MQ=255)

                                                                                                                          ttATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAg                   <  2:923065/141‑1 (MQ=255)

                                                                                                                            aTTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCt                 <  1:1405306/141‑1 (MQ=255)

                                                                                                                               gACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACAATAATTACAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAAt              <  2:764326/141‑1 (MQ=255)

                                                                                                                                 cTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACATTAATACCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCa            <  2:168/141‑1 (MQ=255)

                                                                                                                                     gaTGTTGGTAAAATGGATTATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAAAATTGAAAACAGAATGGCAATATGTCAAAGTTAATTTCCAAAAAAGTAAATATAAATTACAAACATTATTTAGTAATTATGAGCTaaataaaaa        >  2:1151727/2‑134 (MQ=255)

                                                                                                                                       tGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAAcatc      <  1:765328/141‑1 (MQ=255)

                                                                                                                                         ttAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAGAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAAcatcat    <  2:1034094/141‑1 (MQ=255)

                                                                                                                                         ttAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAAcatcat    <  1:705723/141‑1 (MQ=255)

                                                                                                                                           aataaAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATGTAGTATTTATGAGCTAATCAAACATCATaa  <  1:731334/141‑1 (MQ=255)

GGTGTAATTTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAA‑‑‑TGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGCTAATCAAACATCATAA  >  CP000730/512504‑512783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A