Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F70 I0 R1 629 86.3 3068034 98.1% 3009741 142.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 922,314:1 +C 11.1% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3141.C11.1% 236.5 / 28.3 63intergenic (+21/+52)macB/cspDmacrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base . (25/31);  new base C (4/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGC  >  NC_000913/922166‑922461
                                                                                                                                                     |                                                                                                                                                   
ttGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAg                                                                                                                                                      >  1:347638/1‑149 (MQ=255)
  ggTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑cc                                                                                                                                                   >  3:150520/1‑149 (MQ=255)
    tttttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGa                                                                                                                                                 >  3:447413/1‑149 (MQ=255)
        tCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCAAGACATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAAGGCCAG‑CCGATCgc                                                                                                                                             <  4:491584/149‑2 (MQ=255)
            cGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCggctg                                                                                                                                          <  4:269579/149‑4 (MQ=255)
                ggCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATC‑GGCTGGCAt                                                                                                                                     <  4:354028/149‑1 (MQ=255)
                     tgctgctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGc                                                                                                                                >  1:116492/1‑149 (MQ=255)
                        tgctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCtt                                                                                                                             >  1:100539/1‑149 (MQ=255)
                        tgctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCtt                                                                                                                             >  1:353122/1‑149 (MQ=255)
                         gctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCttt                                                                                                                            <  2:46286/149‑1 (MQ=255)
                          ctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTa                                                                                                                           <  1:167800/149‑1 (MQ=255)
                          ctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTa                                                                                                                           <  2:313108/149‑1 (MQ=255)
                           tgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAg                                                                                                                          <  4:440376/149‑1 (MQ=255)
                                tttCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGt                                                                                                                     <  4:588374/149‑1 (MQ=255)
                                 ttCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTa                                                                                                                    >  3:530327/1‑149 (MQ=255)
                                    tctGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTacac                                                                                                                 >  4:201749/1‑149 (MQ=255)
                                      tGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACaa                                                                                                               <  2:743395/149‑1 (MQ=255)
                                        cTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATg                                                                                                             >  3:324071/1‑149 (MQ=255)
                                         tCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATga                                                                                                            <  1:234850/149‑1 (MQ=255)
                                         tCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATga                                                                                                            <  1:317796/149‑1 (MQ=255)
                                                  aCCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                                                                   >  4:217233/1‑149 (MQ=255)
                                                   ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAagag                                                                                                  >  3:740323/1‑149 (MQ=255)
                                                    cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGc                                                                                                 >  1:318134/1‑149 (MQ=255)
                                                       gATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTAt                                                                                              <  3:553677/149‑1 (MQ=255)
                                                        aTTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATg                                                                                             <  1:221311/149‑1 (MQ=255)
                                                         ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGc                                                                                            <  1:170432/149‑1 (MQ=255)
                                                          tttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCg                                                                                           >  1:510680/1‑149 (MQ=255)
                                                             aTTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACt                                                                                        >  3:542118/1‑149 (MQ=255)
                                                                  gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGc                                                                                   <  2:353122/149‑1 (MQ=255)
                                                                  gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGc                                                                                   <  2:100539/149‑1 (MQ=255)
                                                                        tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTAc                                                                             >  3:142435/1‑149 (MQ=255)
                                                                           ccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                                                                          <  2:552440/149‑1 (MQ=255)
                                                                             gCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGa                                                                        >  4:721341/1‑149 (MQ=255)
                                                                                         gCACGACTGGATCCAGTAGATACTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa                                                            <  4:548782/149‑1 (MQ=255)
                                                                                           aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAaca                                                          <  2:116492/149‑1 (MQ=255)
                                                                                             gACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt                                                        >  4:700141/1‑149 (MQ=255)
                                                                                             gACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt                                                        >  2:185375/1‑149 (MQ=255)
                                                                                               cTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg                                                      <  1:252231/149‑1 (MQ=255)
                                                                                                  gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGt                                                   >  3:723332/1‑149 (MQ=255)
                                                                                                   aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTg                                                  >  1:122222/1‑149 (MQ=255)
                                                                                                    tCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGa                                                 <  3:3239/149‑1 (MQ=255)
                                                                                                     ccAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGAt                                                <  2:7575/149‑1 (MQ=255)
                                                                                                       aGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTATTTCGACGGGCACAATAACACTGGCGTGATTg                                              <  3:700141/149‑1 (MQ=255)
                                                                                                       aGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTg                                              >  3:662968/1‑149 (MQ=255)
                                                                                                        gTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGc                                             <  4:447413/149‑1 (MQ=255)
                                                                                                             tGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCtttt                                        <  1:115670/149‑1 (MQ=255)
                                                                                                               ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTgg                                      >  3:658350/1‑149 (MQ=255)
                                                                                                               ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTgg                                      >  3:735353/1‑149 (MQ=255)
                                                                                                                tctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTTTGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                     >  3:717713/1‑149 (MQ=255)
                                                                                                                        gagaGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggtg                             <  4:150520/149‑1 (MQ=255)
                                                                                                                              aaTTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATc                       <  4:658350/149‑1 (MQ=255)
                                                                                                                                tttttGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCaa                     >  4:297032/1‑149 (MQ=255)
                                                                                                                                 ttttGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCaaa                    <  2:347638/149‑1 (MQ=255)
                                                                                                                                   ttGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACACTGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACt                  <  4:735353/149‑1 (MQ=255)
                                                                                                                                      agaTAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGaa               <  3:114526/149‑1 (MQ=255)
                                                                                                                                        aTAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACg             >  2:102892/1‑149 (MQ=255)
                                                                                                                                           aaaaTGCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGAt          >  3:383920/1‑149 (MQ=255)
                                                                                                                                           aaaaTGCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGAt          >  1:661488/1‑149 (MQ=255)
                                                                                                                                           aaaaTGCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAAGGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCTCTGGTGGACATCAAACTGAACGGAt          >  2:450823/1‑149 (MQ=255)
                                                                                                                                           aaaaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAGCGGAt          <  3:450642/149‑1 (MQ=255)
                                                                                                                                           aaaaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGAt          <  1:185375/149‑1 (MQ=255)
                                                                                                                                             aaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTg        >  1:110725/1‑149 (MQ=255)
                                                                                                                                              aTGCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACa                         >  4:229430/1‑131 (MQ=255)
                                                                                                                                              aTGCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACa                         <  3:229430/131‑1 (MQ=255)
                                                                                                                                                   aG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGc  <  3:721341/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGC  >  NC_000913/922166‑922461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A