Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F25 I0 R2 821 102.6 3645220 97.9% 3568670 140.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 922,314:1 +C 8.2% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3141.C 8.2% 297.3 / 23.3 73intergenic (+21/+52)macB/cspDmacrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base . (33/34);  new base C (4/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

TTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCT  >  NC_000913/922166‑922462
                                                                                                                                                     |                                                                                                                                                    
ttGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAg                                                                                                                                                       >  4:469293/1‑149 (MQ=255)
 tGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAg                                                                                                                                                       <  2:11494/148‑1 (MQ=255)
 tGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑c                                                                                                                                                     <  3:346218/149‑1 (MQ=255)
   gTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑cc                                                                                                                                                    >  1:71860/1‑148 (MQ=255)
    tttttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGa                                                                                                                                                  >  4:30031/1‑149 (MQ=255)
     ttttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGGGTAATTTTTGAGATAAAAATGCCAG‑CCGAt                                                                                                                                                 <  3:535344/149‑1 (MQ=255)
         cACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCgg                                                                                                                                              >  2:253563/1‑148 (MQ=255)
          aCCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGATACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGc                                                                                                                                            >  4:350080/1‑149 (MQ=255)
             gTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTgg                                                                                                                                         >  4:526217/1‑149 (MQ=255)
               tGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCa                                                                                                                                       <  3:104229/149‑1 (MQ=255)
                   gctgctgctGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCAttt                                                                                                                                    <  2:113626/148‑1 (MQ=255)
                     tgctgctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTg                                                                                                                                  <  2:184421/148‑1 (MQ=255)
                          ctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTa                                                                                                                            <  3:23679/149‑1 (MQ=255)
                           tgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTa                                                                                                                            >  2:729476/1‑148 (MQ=255)
                           tgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAg                                                                                                                           <  3:893310/149‑1 (MQ=255)
                             gCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGa                                                                                                                         >  3:284342/1‑149 (MQ=255)
                              cGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGa                                                                                                                                                  <  2:759236/123‑1 (MQ=255)
                              cGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGa                                                                                                                                                  >  1:759236/1‑123 (MQ=255)
                                tttCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATg                                                                                                                       >  1:576976/1‑148 (MQ=255)
                                     ctGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTacac                                                                                                                  >  2:687531/1‑148 (MQ=255)
                                      tGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACaa                                                                                                                >  4:416929/1‑149 (MQ=255)
                                        cTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATg                                                                                                              >  3:632849/1‑149 (MQ=255)
                                           gACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGc                                                                                                                                 >  4:805626/1‑127 (MQ=255)
                                           gACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGc                                                                                                                                 <  3:805626/127‑1 (MQ=255)
                                                   ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAagag                                                                                                   <  3:469293/149‑1 (MQ=255)
                                                    cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATC‑GGCTGGCAt                                                                                                                                      <  1:335669/113‑1 (MQ=255)
                                                    cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATC‑GGCTGGCAt                                                                                                                                      >  2:335669/1‑113 (MQ=255)
                                                    cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTa                                                                                                                            >  4:246609/1‑123 (MQ=255)
                                                    cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTa                                                                                                                            <  3:246609/123‑1 (MQ=255)
                                                    cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGc                                                                                                  >  3:213161/1‑149 (MQ=255)
                                                                   cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGGCAGAAGAGCTATGCGACTGCCGc                                                                                    >  2:249914/1‑148 (MQ=255)
                                                                    tGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATC‑GGCTGGc                                                                                                                                        <  4:707826/95‑1 (MQ=255)
                                                                    tGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATC‑GGCTGGc                                                                                                                                        >  3:707826/1‑95 (MQ=255)
                                                                     ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCtt                                                                                  >  1:871673/1‑148 (MQ=255)
                                                                      gTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                                                                                >  4:143152/1‑149 (MQ=255)
                                                                      gTTACACGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                                                                                <  3:526217/149‑1 (MQ=255)
                                                                        tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGGCAGAAGAGCTATGCGACTGCCGCTTCTa                                                                               <  1:249914/148‑1 (MQ=255)
                                                                        tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTa                                                                               <  2:576976/148‑1 (MQ=255)
                                                                        tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTAc                                                                              <  4:430116/149‑1 (MQ=255)
                                                                        tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTAc                                                                              <  4:805970/149‑1 (MQ=255)
                                                                          cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACtt                                                                            <  4:632849/149‑1 (MQ=255)
                                                                          cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACt                                                                             <  1:253563/148‑1 (MQ=255)
                                                                              cACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGAc                                                                        <  4:626198/149‑1 (MQ=255)
                                                                               aCGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGAc                                                                        >  1:313320/1‑148 (MQ=255)
                                                                               aCGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACg                                                                       >  3:719698/1‑149 (MQ=255)
                                                                                cGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTAcaca                                                                                                                 <  1:897012/106‑1 (MQ=255)
                                                                                cGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTAcaca                                                                                                                 >  2:897012/1‑106 (MQ=255)
                                                                                      gCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCAtt                                                                                                                                     >  2:734853/1‑80 (MQ=255)
                                                                                      gCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCAtt                                                                                                                                     <  1:734853/80‑1 (MQ=255)
                                                                                      gCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACaa                                                                <  3:416929/149‑1 (MQ=255)
                                                                                         gCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa                                                             >  3:326398/1‑149 (MQ=255)
                                                                                                tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGc                                                      <  4:219398/149‑1 (MQ=255)
                                                                                                      cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGAtt                                                <  4:284342/149‑1 (MQ=255)
                                                                                                      cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGAt                                                 <  1:687531/148‑1 (MQ=255)
                                                                                                         tAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGc                                              >  2:764472/1‑148 (MQ=255)
                                                                                                             tGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCttt                                          <  1:30328/148‑1 (MQ=255)
                                                                                                             tGCTCTGGCACGAGAGTAATTTCTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCatt                                          <  1:783663/148‑1 (MQ=255)
                                                                                                               ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTg                                        >  1:358108/1‑148 (MQ=255)
                                                                                                                tctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                      >  3:598206/1‑149 (MQ=255)
                                                                                                                 ctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGa                                                                                           <  2:754276/95‑1 (MQ=255)
                                                                                                                 ctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGa                                                                                           >  1:754276/1‑95 (MQ=255)
                                                                                                                 ctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                      >  1:355880/1‑148 (MQ=255)
                                                                                                                       cGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcccc                                   >  1:153265/1‑145 (MQ=255)
                                                                                                                       cGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcccc                                   <  2:153265/145‑1 (MQ=255)
                                                                                                                       cGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt                               <  3:350080/149‑1 (MQ=255)
                                                                                                                       cGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtgg                                <  2:358108/148‑1 (MQ=255)
                                                                                                                             tAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACAt                         >  3:77085/1‑149 (MQ=255)
                                                                                                                                        aTAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACaa                                                                                                                <  4:429592/51‑1 (MQ=255)
                                                                                                                                        aTAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACaa                                                                                                                >  3:429592/1‑51 (MQ=255)
                                                                                                                                          aaaaaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACgg             <  2:71860/148‑1 (MQ=255)
                                                                                                                                           aaaaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATa                                                              >  1:656188/1‑98 (MQ=255)
                                                                                                                                           aaaaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATa                                                              <  2:656188/98‑1 (MQ=255)
                                                                                                                                            aaaTGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGAtt          >  3:556338/1‑149 (MQ=255)
                                                                                                                                             aaTGCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGAtt          >  2:332124/1‑148 (MQ=255)
                                                                                                                                                gCCAGCCCGATC‑GGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTc       >  2:741045/1‑148 (MQ=255)
                                                                                                                                                   aG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCg                                                                                     >  1:653126/1‑67 (MQ=255)
                                                                                                                                                   aG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCg                                                                                     <  2:653126/67‑1 (MQ=255)
                                                                                                                                                    g‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCt  >  3:204816/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAG‑CCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCT  >  NC_000913/922166‑922462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A