Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F5 I0 R1
|
408 |
67.6 |
2494072 |
96.8% |
2414261 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,763,450 |
A→G |
7.7% |
V187A (GTG→GCG) ‡ |
sufB ← |
component of SufBCD Fe‑S cluster assembly scaffold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,763,450 | 0 | A | G | 7.7%
| 158.4
/ 3.6
| 52 | V187A (GTG→GCG) ‡ | sufB | component of SufBCD Fe‑S cluster assembly scaffold |
| Reads supporting (aligned to +/- strand): ref base A (25/23); new base G (2/2); total (27/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 |
TCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCC > NC_000913/1763303‑1763590
|
tCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGcaccac < 1:207875/149‑1 (MQ=255)
cTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGc < 3:336273/149‑1 (MQ=255)
gCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCg < 1:605115/149‑1 (MQ=255)
gTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGata > 4:265721/1‑149 (MQ=255)
gTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGata > 3:51529/1‑149 (MQ=255)
tttttCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATAtt > 2:111997/1‑149 (MQ=255)
tGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCa > 4:128794/1‑149 (MQ=255)
cGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCgg < 3:265721/149‑1 (MQ=255)
tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTgg > 2:578688/1‑149 (MQ=255)
ggAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTg > 1:117584/1‑147 (MQ=255)
ggAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTg < 2:117584/147‑1 (MQ=255)
ggAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGa > 2:578796/1‑149 (MQ=255)
ttCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCt > 1:215309/1‑149 (MQ=255)
cATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCAc < 4:81063/149‑1 (MQ=255)
aGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGaa > 1:520787/1‑149 (MQ=255)
aGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGaa < 3:456830/149‑1 (MQ=255)
gcgcACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc < 2:520787/149‑1 (MQ=255)
gCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaaaa > 2:511510/1‑149 (MQ=255)
cTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaata > 4:607193/1‑149 (MQ=255)
tttAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataa < 4:96592/149‑1 (MQ=255)
tAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGGACAGAAAATAAtt < 4:68922/149‑1 (MQ=255)
aGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCa > 1:151311/1‑98 (MQ=255)
aGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCa < 2:151311/98‑1 (MQ=255)
ggCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTcc < 1:261060/149‑1 (MQ=255)
acaTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTg > 1:291947/1‑149 (MQ=255)
acaTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTg > 1:464559/1‑149 (MQ=255)
ataaaCGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGc > 1:565190/1‑149 (MQ=255)
cGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGtt < 1:511510/149‑1 (MQ=255)
cATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcg > 3:67487/1‑149 (MQ=255)
aTCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgc < 2:565190/149‑1 (MQ=255)
cAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGa > 3:67481/1‑149 (MQ=255)
agagGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt < 1:578688/149‑1 (MQ=255)
agGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATaa < 2:291947/149‑1 (MQ=255)
aCCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagtagt < 4:67487/149‑1 (MQ=255)
ccgccgCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTg > 2:563063/1‑149 (MQ=255)
gccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGc > 2:26135/1‑149 (MQ=255)
cgGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAg < 3:235433/149‑1 (MQ=255)
ggCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGt < 3:128794/149‑1 (MQ=255)
gaagTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa < 2:215309/149‑1 (MQ=255)
aagTTGTCATTCCCCGGCACCACGGTGCCGAGATAGTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAAt > 4:383751/1‑149 (MQ=255)
agTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATg < 3:129910/149‑1 (MQ=255)
agTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATg < 4:51529/149‑1 (MQ=255)
tGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGACTCGAAAATGGCa > 2:212390/1‑149 (MQ=255)
gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa < 3:227765/143‑1 (MQ=255)
gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa > 4:227765/1‑143 (MQ=255)
ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCAc > 1:242233/1‑149 (MQ=255)
tCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataa > 1:433117/1‑81 (MQ=255)
tCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTCGATCGCCTCACCCAAGGAACAGAAaagaa < 2:433117/81‑1 (MQ=255)
atctcGGCACCGTGGTGCCGGGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa > 4:340688/5‑138 (MQ=255)
atctcGGCACCGTGGTGCCGGGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa < 3:340688/134‑1 (MQ=255)
tcGGCACCGTGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTccc < 1:419128/82‑1 (MQ=255)
tcGGCACCGTGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTccc > 2:419128/2‑83 (MQ=255)
ccGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGcc > 1:521802/1‑149 (MQ=255)
|
TCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCC > NC_000913/1763303‑1763590
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
