Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F108 I0 R1 889 70.1 2273064 96.2% 2186687 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,823,577 C→A 31.8% P393Q (CCG→CAG)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,823,5770CA31.8% 86.7 / 62.5 82P393Q (CCG→CAG) spoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (38/18);  new base A (12/14);  total (50/32)
Fisher's exact test for biased strand distribution p-value = 8.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

CACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACC  >  NC_000913/3823429‑3823725
                                                                                                                                                    |                                                                                                                                                    
cACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGCGCGCGTTAAATCCGATCTCTTCCCGGATGCGATTTACGTTTTCACAcc                                                                                                                                                      >  2:985222/1‑149 (MQ=255)
   ggCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGATTTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGa                                                                                                                                                   >  1:1009856/1‑149 (MQ=255)
    gCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGaa                                                                                                                                                  >  2:654885/1‑149 (MQ=255)
      gAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAgg                                                                                                                                                >  1:299094/1‑149 (MQ=255)
        aaCCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGgc                                                                                                                                              <  2:311023/149‑1 (MQ=255)
        aaCCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGgc                                                                                                                                              <  1:171316/149‑1 (MQ=255)
          ccAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGgcgc                                                                                                                                            >  1:21717/1‑149 (MQ=255)
           cAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAGCAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCa                                                                                                                                           >  1:18446/1‑149 (MQ=255)
                ctacCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTc                                                                                                                                      >  1:198088/1‑149 (MQ=255)
                 tacCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCg                                                                                                                                     >  2:396716/1‑149 (MQ=255)
                 tacCGCACAAATCAGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCTGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGAGCTCTTCCCGGATGAGATTTACGTTTTCAAACAGGAAGGGCGCAGTGTcc                                                                                                                                     >  1:90799/1‑148 (MQ=255)
                  cccGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGa                                                                                                                                    <  2:985700/148‑1 (MQ=255)
                  acCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGa                                                                                                                                    <  2:102775/149‑1 (MQ=255)
                   ccGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAg                                                                                                                                   >  2:925034/1‑149 (MQ=255)
                   ccGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAg                                                                                                                                   >  2:705507/1‑149 (MQ=255)
                   ccGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAg                                                                                                                                   >  1:12861/1‑149 (MQ=255)
                     gCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGct                                                                                                                                 >  1:151289/1‑149 (MQ=255)
                      cacaAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGctg                                                                                                                                <  1:629613/149‑1 (MQ=255)
                         aaaTCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGCGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGCAAGGGCGCATTGTCGAGctgcca                                                                                                                             >  1:310271/1‑148 (MQ=255)
                         aaaTCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGctgcct                                                                                                                             >  1:796900/1‑149 (MQ=255)
                           aTCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGctgcctgc                                                                                                                           <  1:298197/149‑1 (MQ=255)
                           aTCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGctgcctgc                                                                                                                           <  1:867268/149‑1 (MQ=255)
                             ccGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCg                                                                                                                         >  1:245400/1‑149 (MQ=255)
                                 gcCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGc                                                                                                                     <  2:376902/149‑1 (MQ=255)
                                 gcCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGc                                                                                                                     >  1:416651/1‑149 (MQ=255)
                                   ccAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCaa                                                                                                                   >  1:318647/1‑149 (MQ=255)
                                   ccAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCaa                                                                                                                   <  2:11674/149‑1 (MQ=255)
                                         cTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCg                                                                                                             >  2:119204/1‑149 (MQ=255)
                                           ggATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTc                                                                                                           <  2:732845/149‑1 (MQ=255)
                                            gATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCg                                                                                                          >  2:587157/1‑149 (MQ=255)
                                            gATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCg                                                                                                          >  2:498619/1‑149 (MQ=255)
                                             aTGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGa                                                                                                         <  1:1110815/149‑1 (MQ=255)
                                                cAAAGCCTGCTGGAGCTGCAACCGAGCGCCGGTAGGTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACtt                                                                                                      <  2:726671/149‑1 (MQ=255)
                                                cAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACtt                                                                                                      <  1:296259/149‑1 (MQ=255)
                                                cAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACtt                                                                                                      >  1:822550/1‑149 (MQ=255)
                                                  aaGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCg                                                                                                    >  2:1049771/1‑149 (MQ=255)
                                                     ccTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGCGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCtt                                                                                                 <  1:864032/149‑1 (MQ=255)
                                                      ctgctgGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTa                                                                                                <  2:678330/149‑1 (MQ=255)
                                                            gAGCTGCAGCAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGt                                                                                          <  2:18446/149‑1 (MQ=255)
                                                              gCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGc                                                                                        >  1:861018/1‑149 (MQ=255)
                                                                tGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCAt                                                                                      >  1:793274/1‑149 (MQ=255)
                                                                tGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCAt                                                                                      >  1:194043/1‑149 (MQ=255)
                                                                 gCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATa                                                                                     >  1:244231/1‑149 (MQ=255)
                                                                   aaCAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGCCTTCGCTTATGCAGTGCATAcc                                                                                   >  2:350102/1‑149 (MQ=255)
                                                                   aaCAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCAGTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATAcc                                                                                   >  1:393849/1‑149 (MQ=255)
                                                                    aCAGAGCGCCGGTAGTTCGTTTGAATTAATCGAGAGCGTTAAATCCGATCTCTTCCAGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTCCCGGTGAAACGCCCGTCGACTTCGCTTATGCAGTTCATACCg                                                                                  >  2:337359/1‑149 (MQ=255)
                                                                        agCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGatat                                                                              <  2:688241/149‑1 (MQ=255)
                                                                           gcCGGTAGATCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCgg                                                                           <  2:116078/149‑1 (MQ=255)
                                                                            ccGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGt                                                                          <  2:950647/149‑1 (MQ=255)
                                                                             cGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTc                                                                         >  2:1044928/1‑149 (MQ=255)
                                                                             cGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTc                                                                         >  1:475724/1‑149 (MQ=255)
                                                                               gTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCAt                                                                       >  1:1123755/1‑149 (MQ=255)
                                                                                 aGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGc                                                                     <  2:526470/149‑1 (MQ=255)
                                                                                  gTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGcc                                                                    >  1:633256/1‑149 (MQ=255)
                                                                                       tttGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGt                                                               >  1:476975/1‑149 (MQ=255)
                                                                                          gAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTggg                                                            <  1:365404/149‑1 (MQ=255)
                                                                                           aaTTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCCCCCCGGAAGGGCGCATTGTCTAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGGCATGCCTGCGTGGgc                                                           >  2:752575/1‑149 (MQ=255)
                                                                                             tttATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGgcgc                                                         >  2:621495/1‑149 (MQ=255)
                                                                                                 tCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCAcac                                                     <  2:1123755/149‑3 (MQ=255)
                                                                                                    agagCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGtt                                                  >  1:245257/1‑149 (MQ=255)
                                                                                                    agagCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGtt                                                  >  2:528737/1‑149 (MQ=255)
                                                                                                    agagCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGtt                                                  >  1:684967/1‑149 (MQ=255)
                                                                                                    agagCGTTAAATCCGAGCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGtt                                                  <  2:97673/149‑1 (MQ=255)
                                                                                                        cGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGAcc                                              >  2:80804/1‑149 (MQ=255)
                                                                                                            aaaTCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCa                                          <  1:396716/149‑1 (MQ=255)
                                                                                                              aTCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGc                                        >  1:229224/1‑149 (MQ=255)
                                                                                                              aTCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGACGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCCCACGCGTTGACCGCCAGc                                        >  2:353090/1‑149 (MQ=255)
                                                                                                               tCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGcc                                       <  1:916179/149‑1 (MQ=255)
                                                                                                                  gATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTa                                    >  1:7163/1‑149 (MQ=255)
                                                                                                                   aTCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTAc                                   <  1:1049771/149‑1 (MQ=255)
                                                                                                                   aTCTCTTCCCGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTAc                                   <  2:198088/149‑1 (MQ=255)
                                                                                                                    tctcTTCCCGGATGAGATTTACGGTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTAcc                                  <  1:253322/149‑1 (MQ=255)
                                                                                                                          cccGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGt                            >  2:876771/1‑149 (MQ=255)
                                                                                                                            cGGATGAGATTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTAGGCGCTCGCGTTGACCGCCAGCCTTACCCGCTGTCg                          >  2:711311/1‑149 (MQ=255)
                                                                                                                            cGGATGAGATTTACGTTTACACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCg                          >  2:256102/1‑149 (MQ=255)
                                                                                                                                  agaTTTACGTTTTCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCg                    <  2:194043/149‑1 (MQ=255)
                                                                                                                                      ttACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTa                <  1:575429/149‑1 (MQ=255)
                                                                                                                                          gTTTTCACACCGGAAGGGCGCATTGTCGNGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAg            >  2:695031/1‑149 (MQ=255)
                                                                                                                                          gTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAg            >  2:792462/1‑149 (MQ=255)
                                                                                                                                            tttCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCg          <  2:579413/149‑1 (MQ=255)
                                                                                                                                              tCACACAGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGt        <  2:416651/149‑1 (MQ=255)
                                                                                                                                                acacCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCCTGCCTGCGTGGGCGCACGCGTTGACAGCCAGCCTTACCCGCTGTCGCAGCCACTTACCAGCGGTCa      >  1:585920/1‑149 (MQ=255)
                                                                                                                                                acacCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCa      >  1:770362/1‑149 (MQ=255)
                                                                                                                                                    cGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGCCCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAAcc  >  2:730315/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACC  >  NC_000913/3823429‑3823725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A