Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F18 I0 R1	32	69.1	1533550	96.6%	1481409	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,644	C→A	22.8%	V10F (GTT→TTT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,644	0	C	A	22.8%	132.8 / 30.7	68	V10F (GTT→TTT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (34/18); new base A (8/8); total (42/26)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACGTTGTTGTCTTAATT  >  USA300TCH1516_ALE/1744506‑1744784

cAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACtt                                                                                                                                            >  2:76325/1‑141 (MQ=255)

cAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACtt                                                                                                                                            >  1:230372/1‑141 (MQ=255)

 aGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTc                                                                                                                                           <  2:385369/141‑1 (MQ=255)

       tACAACGATACCAACATCTCTGGCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATAc                                                                                                                                     <  2:895/141‑1 (MQ=255)

        ataaCGATATCAACATCTCTGTCTTTTGCGTATTCTTCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACg                                                                                                                                    <  1:577620/139‑1 (MQ=255)

            cGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTAtt                                                                                                                                >  2:50505/1‑141 (MQ=255)

             gATATCAACATCTCTGTCTTTTTCTTATTCTACAATTTTAACTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTg                                                                                                                               >  2:371454/1‑141 (MQ=255)

              atatCAACATCTCTGTCTTTTGCGTATTTTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGc                                                                                                                              <  1:392205/141‑1 (MQ=255)

                atCAACATCTTTGTTTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCtt                                                                                                                            <  2:244787/141‑1 (MQ=255)

                 tCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCttt                                                                                                                           <  1:272676/141‑1 (MQ=255)

                          cttgcTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCAtt                                                                                                                  <  2:643957/137‑1 (MQ=255)

                          ctGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCAtt                                                                                                                  >  2:117744/1‑141 (MQ=255)

                                    cGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATTCATTAATATTtcct                                                                                                        >  1:449438/1‑141 (MQ=255)

                                      tATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTtcctcc                                                                                                      >  2:427643/1‑141 (MQ=255)

                                        ttCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCCTTATCCCTAATTCTAGTAATACCCTTGAAACTAACACCCGGTTTCGGCCCATCTTGAACTTCTGATACGTATTGCTTTCAATCCATTAATATTTcctcctc                                                                                                    >  2:306378/1‑140 (MQ=255)

                                        ttCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTa                                                                                                    >  2:306377/1‑141 (MQ=255)

                                               aTTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCAATTAATACTCCCTCCTAAATTGCt                                                                                             <  2:757760/141‑1 (MQ=255)

                                                   tATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACg                                                                                         <  1:67742/141‑1 (MQ=255)

                                                    aTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGa                                                                                        >  2:318799/1‑141 (MQ=255)

                                                    aTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGATCACGa                                                                                        >  2:203650/1‑141 (MQ=255)

                                                    aTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATACATTACTATTTCCTCCTAAATTGCTCACGa                                                                                        >  1:80636/1‑141 (MQ=255)

                                                     tCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTCAATCCATTAATATTTTCTCCTAAATTGCTCACGAc                                                                                       >  2:628262/1‑141 (MQ=255)

                                                       ttttGCATAGCCATATGCATCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACaa                                                                                     <  1:489003/139‑1 (MQ=255)

                                                        gTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCCAACTTGAAATTCTGCTACGACTTGCTTTACATCCATTAATATTTCCTCCTAAATTGCTCCCgcccat                                                                                    >  2:594082/1‑136 (MQ=255)

                                                        gTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAACCCATTAATATTTCCTCCTAAATTGCTCACGACAAt                                                                                    <  1:371454/141‑1 (MQ=255)

                                                          tGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATtg                                                                                  <  1:3176/141‑1 (MQ=255)

                                                           gCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATtgt                                                                                 <  1:210249/141‑1 (MQ=255)

                                                                gCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACtt                                                                            >  2:446681/1‑141 (MQ=255)

                                                                  cATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTACTATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTa                                                                          >  1:590284/1‑141 (MQ=255)

                                                                     atGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCTTCCTAAATTGCTCACGACAATTGTGACTTTATcc                                                                       >  2:645072/1‑141 (MQ=255)

                                                                     atGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATcc                                                                       >  2:228312/1‑141 (MQ=255)

                                                                      tGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCa                                                                      >  2:632319/1‑141 (MQ=255)

                                                                       gCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCaa                                                                     >  2:50391/1‑141 (MQ=255)

                                                                        cTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAt                                                                    >  1:546287/1‑141 (MQ=255)

                                                                           cACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAtttc                                                                 <  1:214877/141‑2 (MQ=255)

                                                                            aCCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAttttt                                                                >  2:294459/1‑141 (MQ=255)

                                                                              cATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCATCCTAAATTGCTCACGCCAATTGTGACTCTATCCAAttttttt                                                              >  2:765658/1‑140 (MQ=255)

                                                                              cATTATCCATAATTGTAGGAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTa                                                              <  1:380375/141‑1 (MQ=255)

                                                                                ttATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCAAGACAATTGTGACTTTATCCAATTTTTTAtt                                                            >  1:658561/1‑141 (MQ=255)

                                                                                 tATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTAttt                                                           >  2:736855/1‑141 (MQ=255)

                                                                                     cATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGa                                                       >  1:389402/1‑141 (MQ=255)

                                                                                      aTAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGACCAATCTTGAACTTCTGATACGCATTGCTTTAAATCCATTAATTTTTCCTCCTAAATTGCTCCCGACAATTGTGACTTTATCCAATTTTTTATTTCTGaa                                                      >  2:269329/1‑141 (MQ=255)

                                                                                       tAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGTCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaa                                                     >  1:647734/1‑141 (MQ=255)

                                                                                        aaTTGGAGTAATATCCTTGAAACTAACACCTGGTTTCCGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACACTTGTGACTTTATCCAATTTTTTATTTCTTaaaa                                                    >  2:299122/1‑141 (MQ=255)

                                                                                           tGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATc                                                 >  1:603935/1‑141 (MQ=255)

                                                                                            gtagtaATATCCTTGATACCAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCATAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCt                                                >  1:429356/1‑141 (MQ=255)

                                                                                               gtaATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTATATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGa                                             >  1:176230/1‑141 (MQ=255)

                                                                                               gtaATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATGTCTGAAAAATCTTGa                                             >  1:329042/1‑141 (MQ=255)

                                                                                               gtaATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATCGTGACTTTATCCAATTTTTTAGTTCTGAAAAATCTTGa                                             <  1:427643/141‑1 (MQ=255)

                                                                                                 aaTATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTAGACCAAAGATGGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGata                                           >  1:365680/1‑141 (MQ=255)

                                                                                                       cTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATAtaataa                                     >  2:727521/1‑141 (MQ=255)

                                                                                                        ttGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATaataat                                    <  2:133135/141‑1 (MQ=255)

                                                                                                            aaCTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAAGCCAGTAATATTTCCTCCTAAGTTGCTCACGGCCATGGGGACTGTATCCAATTTTTTATTTCTGAAATATCTTGATATaataatgtct                                >  1:333369/1‑137 (MQ=255)

                                                                                                             aCTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCtt                               <  2:452296/141‑1 (MQ=255)

                                                                                                             aCTAACACCTGGTGTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCtt                               <  2:59413/141‑1 (MQ=255)

                                                                                                                aaCACCTGGTTTCGGCCAATCTTAAAAGCCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTc                            >  2:165473/1‑141 (MQ=255)

                                                                                                                 acacCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTACTATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCa                           >  1:105967/1‑141 (MQ=255)

                                                                                                                 acacCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCa                           >  1:91062/1‑141 (MQ=255)

                                                                                                                     cTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACAt                       <  1:117744/141‑1 (MQ=255)

                                                                                                                     cTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACAt                       <  1:113836/141‑1 (MQ=255)

                                                                                                                      tGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATc                      >  2:607948/1‑141 (MQ=255)

                                                                                                                             ggCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACgt               <  1:529979/141‑1 (MQ=255)

                                                                                                                               ccAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACgttg             <  2:691305/141‑1 (MQ=255)

                                                                                                                                cAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACgttgt            <  1:356866/141‑1 (MQ=255)

                                                                                                                                 aaTCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATCATAATTGCTTTTCAACATCCATACgttgtt           >  1:187441/1‑141 (MQ=255)

                                                                                                                                   tCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACGTtgttgt         <  1:422925/141‑1 (MQ=255)

                                                                                                                                    cTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACGTTGTTGTc        <  1:326409/141‑1 (MQ=255)

                                                                                                                                       gAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGTAATATCTTGATCTAATACTAGCTTTTCAACCTCCATACGTTGTTGTCTTa     >  2:428679/1‑141 (MQ=255)

                                                                                                                                          cTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACGTTGTTGTCTTAAtt  >  1:314326/1‑141 (MQ=255)

CAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAATAATTGCTTTTCAACATCCATACGTTGTTGTCTTAATT  >  USA300TCH1516_ALE/1744506‑1744784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A