Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I0 R1	76	110.5	2404748	96.5%	2320581	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,761,889	C→A	17.4%	D388Y (GAT→TAT)	fpgS ←	Folylpolyglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,761,889	0	C	A	17.4%	235.9 / 33.4	102	D388Y (GAT→TAT)	fpgS	Folylpolyglutamate synthase
Reads supporting (aligned to +/- strand): ref base C (42/41); new base A (12/6); total (54/48)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 108 total aligned reads displayed.

GATTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTCCG  >  USA300TCH1516_ALE/1761750‑1762028

aaTTCTTCATGGCAATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATct                                                                                                                                            <  1:446106/140‑1 (MQ=255)

 aTTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATcta                                                                                                                                           >  2:826521/1‑141 (MQ=255)

  ttcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATctac                                                                                                                                          >  2:1060798/1‑141 (MQ=255)

    cttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATctacta                                                                                                                                        >  2:478977/1‑141 (MQ=255)

     ttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTaa                                                                                                                                       <  1:966585/141‑1 (MQ=255)

     ttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTaa                                                                                                                                       >  1:1162831/1‑141 (MQ=255)

      tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTACTTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTCCTAAt                                                                                                                                      >  1:273328/1‑141 (MQ=255)

      tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAAt                                                                                                                                      <  2:345852/141‑1 (MQ=255)

      tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAAt                                                                                                                                      <  2:60895/141‑1 (MQ=255)

      tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAt                                                                                                                                      >  1:1015515/1‑141 (MQ=255)

      tcATGGCTATTTTAGTTTTAGTTTCATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAAt                                                                                                                                      >  1:761359/1‑141 (MQ=255)

         tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGt                                                                                                                                   >  1:933469/1‑141 (MQ=255)

         tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGt                                                                                                                                   >  2:369317/1‑141 (MQ=255)

         tGGCTATTTTAGTTTAAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGt                                                                                                                                   >  1:891804/1‑141 (MQ=255)

             tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATGGTAttt                                                                                                                               >  1:346484/1‑141 (MQ=255)

                tttagttttagtTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcat                                                                                                                            <  2:401518/141‑1 (MQ=255)

                  tagttttagtttTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGTAATACAGACTACCGGTAATTAGTAATGTATCACCTTGATACTTTTTTATAAATTAAACGTAGTCATCTACTAATTGTGTTtgatct                                                                                                                          >  2:126820/1‑140 (MQ=255)

                   agttttagttttATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTcatcat                                                                                                                         >  2:34141/1‑141 (MQ=255)

                   agttttagttttATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTcatcat                                                                                                                         <  2:1015515/141‑1 (MQ=255)

                   agttttagttttATAATGCTTCAAAGTCTAATTCTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTcatcat                                                                                                                         >  2:1007309/1‑141 (MQ=255)

                       ttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTTATAATTTTTTCTAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATGTTc                                                                                                                     >  1:197172/1‑141 (MQ=255)

                        tagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCa                                                                                                                    <  1:152178/141‑1 (MQ=255)

                             ttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGACATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCAGCTACTAATTGTATTTCATTATTTTCAAtact                                                                                                               >  2:938663/1‑141 (MQ=255)

                             ttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACCTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAAtact                                                                                                               <  1:98182/141‑1 (MQ=255)

                             ttaCAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAAtact                                                                                                               <  2:1107859/141‑1 (MQ=255)

                               actaTGCNTCAAACTTTTATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAAtactac                                                                                                             <  2:15720/138‑1 (MQ=255)

                                taATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATActacc                                                                                                            <  1:979585/141‑1 (MQ=255)

                                taATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacc                                                                                                            <  1:683867/141‑1 (MQ=255)

                                   tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATActacctac                                                                                                         <  2:141274/141‑1 (MQ=255)

                                    gCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACa                                                                                                        >  1:456314/1‑141 (MQ=255)

                                    gCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACtacataca                                                                                                        >  2:814182/1‑141 (MQ=255)

                                    gCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTAACTACa                                                                                                        >  1:475705/1‑141 (MQ=255)

                                     cTTCAAAGTCTAATTTTGATTTAAGTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACaa                                                                                                       >  1:766435/1‑141 (MQ=255)

                                     cTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAACTGTATTTCATCATTTCCTCTACTCCCTTCaa                                                                                                       <  2:1004034/141‑1 (MQ=255)

                                      ttCAAAGTCTAATTTTTATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAAt                                                                                                      <  1:277677/141‑1 (MQ=255)

                                          aaGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATttct                                                                                                  <  2:553148/141‑1 (MQ=255)

                                          aaGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATttct                                                                                                  <  1:1138236/141‑1 (MQ=255)

                                          aaGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTGCAATACTACCTACAATttct                                                                                                  >  2:929828/1‑141 (MQ=255)

                                             tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTtcttct                                                                                               >  2:827769/1‑141 (MQ=255)

                                                   tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                         <  1:598791/141‑1 (MQ=255)

                                                     tGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTaa                                                                                       <  1:526077/141‑1 (MQ=255)

                                                             cTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCg                                                                               <  1:310744/141‑1 (MQ=255)

                                                                 aCTTATGAAATACAGACTACCGGTAATTACTAATGTATCGGCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaa                                                                           >  2:596669/1‑141 (MQ=255)

                                                                   ttATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTACCGCTTTCGGAAAAt                                                                         >  2:204790/1‑141 (MQ=255)

                                                                     aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                       <  1:679852/141‑1 (MQ=255)

                                                                     aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                       >  1:532292/1‑141 (MQ=255)

                                                                     aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                       >  1:465516/1‑141 (MQ=255)

                                                                     aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                       >  1:131483/1‑141 (MQ=255)

                                                                          aTACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTc                                                                  >  1:1083718/1‑141 (MQ=255)

                                                                            aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAg                                                                >  1:1098149/1‑141 (MQ=255)

                                                                            aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAACCAAATTCat                                                                >  2:454954/1‑140 (MQ=255)

                                                                             cAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                               >  1:372804/1‑141 (MQ=255)

                                                                               gACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTg                                                             >  1:180848/1‑141 (MQ=255)

                                                                                aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                            >  1:861229/1‑141 (MQ=255)

                                                                                       gTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAAc                                                     >  1:729162/1‑141 (MQ=255)

                                                                                       gTAATTACTAATGTATCACCTGGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCATTGCATAACGCTCTCGGAAAATCAAATTCAGTTGCATAAAAc                                                     >  2:247695/1‑141 (MQ=255)

                                                                                        tAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACg                                                    <  1:1015532/141‑1 (MQ=255)

                                                                                        tAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACg                                                    <  1:478977/141‑1 (MQ=255)

                                                                                        tAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAAAGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAAGGCTTTCGGAAAATAAAATTCAGTTTCATaaaaag                                                    >  1:422381/1‑139 (MQ=255)

                                                                                         aaTTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGt                                                   <  1:1133947/141‑1 (MQ=255)

                                                                                            tACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATg                                                <  1:1060798/141‑1 (MQ=255)

                                                                                             ccTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTGTCGGAAAATCAAATTCAGTTGCATAAAGCGTATgc                                               <  2:273328/140‑1 (MQ=255)

                                                                                              cTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcg                                              <  2:861229/141‑1 (MQ=255)

                                                                                              cTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcg                                              <  2:952162/141‑1 (MQ=255)

                                                                                              cTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcg                                              <  2:423098/141‑1 (MQ=255)

                                                                                                aaTGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCa                                            >  1:1134101/1‑141 (MQ=255)

                                                                                                    tATCACCTTTATAATTTTTTATAAATTCAACGTAGTCATTTACTTATTGTATTTCATCATTTTCACAACTACCTAGAATTTCTTCTTTGCGTAACCCTTTCGGAAAACCAAATTCAGTTGCATAAAACGTATTCGCAATTa                                        <  2:580168/141‑1 (MQ=255)

                                                                                                      tCACCTTGATAATTTTTTATAAATTCAACGTCGTCATCTACTAATTGTATTTCATCATTTTCAATAATACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCGAATTCAATTGAATACAAAGTATGCGCAATTaaa                                      >  1:153939/1‑141 (MQ=255)

                                                                                                      tCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTaaa                                      >  1:946404/1‑141 (MQ=255)

                                                                                                        aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACt                                    >  2:412146/1‑141 (MQ=255)

                                                                                                             gATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGCAAATCAAATTCAGTTGCATACAACGTATGCGCAATTAAACTTAAAt                               >  1:4189/1‑141 (MQ=255)

                                                                                                             gATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAAt                               >  1:914396/1‑141 (MQ=255)

                                                                                                              aTAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATg                              <  1:826521/141‑1 (MQ=255)

                                                                                                               tAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                             >  1:109757/1‑141 (MQ=255)

                                                                                                               tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                             >  1:1014149/1‑141 (MQ=255)

                                                                                                                 aTTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGttt                           <  2:131483/141‑1 (MQ=255)

                                                                                                                  ttttttATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                          <  2:970638/141‑1 (MQ=255)

                                                                                                                  ttttttATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTACAGTACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                          <  1:34141/141‑1 (MQ=255)

                                                                                                                        ataAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAAAGTTTGACCAtc                    >  2:336034/1‑141 (MQ=255)

                                                                                                                         taAATTCAACGTAGTGATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTGCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtct                   >  2:1054943/1‑141 (MQ=255)

                                                                                                                          aaaTTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACCATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtctc                  <  1:89369/141‑1 (MQ=255)

                                                                                                                          aaaTTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtctc                  <  1:541108/141‑1 (MQ=255)

                                                                                                                          aaaTTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtctc                  <  1:1091261/141‑1 (MQ=255)

                                                                                                                             ttCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATATCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGtt               >  1:795899/1‑141 (MQ=255)

                                                                                                                                 aCGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAGTGTTTGACCATCTCGTTTAtt           >  2:364203/1‑140 (MQ=255)

                                                                                                                                 aCGCAGCCATCTACTAATTGTATTCGATCATTTTCAATACTACCTATAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATc           <  2:56594/141‑1 (MQ=255)

                                                                                                                                   gTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg         >  1:661696/1‑141 (MQ=255)

                                                                                                                                   gTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg         <  2:1098149/141‑1 (MQ=255)

                                                                                                                                   gTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTAACCTTTCGTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg         <  1:1172732/141‑1 (MQ=255)

                                                                                                                                   gTAGTCATCTACTAAGTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg         <  1:152462/141‑1 (MQ=255)

                                                                                                                                   gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg         >  2:857420/1‑141 (MQ=255)

                                                                                                                                     aGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGtt       >  2:38777/1‑141 (MQ=255)

                                                                                                                                      gTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGttt      <  2:343592/141‑1 (MQ=255)

                                                                                                                                      gTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGttt      >  1:49022/1‑141 (MQ=255)

                                                                                                                                       tCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGtttt     >  2:825770/1‑141 (MQ=255)

                                                                                                                                        cATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCACTTAAACTTCAATGTTATACCAGCTCGTTAATCgagtgtc    >  2:835671/1‑135 (MQ=255)

                                                                                                                                         aTCTACTACTTGTATTTCATCATTTTCAATACCACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTcc   <  1:412898/141‑1 (MQ=255)

                                                                                                                                          tCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGTTTTTCCg  >  2:245930/1‑141 (MQ=255)

                                                                                                                                          tCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTCCg  <  2:840791/141‑1 (MQ=255)

                                                                                                                                          tCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTCCg  <  1:178488/141‑1 (MQ=255)

GATTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTCCG  >  USA300TCH1516_ALE/1761750‑1762028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A