Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I1 R1	31	90.0	1985866	95.2%	1890544	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,105,994	T→C	T12T (ACT→ACC)	pdhD →	Dihydrolipoyl dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,105,994	0	T	C	97.5%	252.0 / NA	81	T12T (ACT→ACC)	pdhD	Dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (50/28); total (52/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATT  >  USA300TCH1516_ALE/1105858‑1106131

ttGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAg                                                                                                                                       <  1:95042/141‑1 (MQ=255)

atGACCCCCGAAAAATTGATGTTTTAACGGGCTAAATTGCAATTAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAg                                                                                                                                       <  2:679120/140‑1 (MQ=255)

      acaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATGGTAATCg                                                                                                                                 >  2:785156/1‑141 (MQ=255)

      acaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCg                                                                                                                                 >  1:120242/1‑141 (MQ=255)

        aGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATCGTAATCggg                                                                                                                               >  1:832554/1‑140 (MQ=255)

        aGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTTATCggg                                                                                                                               >  2:957937/1‑140 (MQ=255)

        aGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGa                                                                                                                               >  2:857856/1‑141 (MQ=255)

        aGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGGAATCGGa                                                                                                                               >  1:865157/1‑141 (MQ=255)

               ttGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTACATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTc                                                                                                                        <  2:648032/141‑1 (MQ=255)

                   tGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCtgg                                                                                                                    >  2:965522/1‑141 (MQ=255)

                     gTGCAACTGGCCAAAATGCAATGAATCACCTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCtggtg                                                                                                                  <  2:336449/141‑1 (MQ=255)

                     gTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTACATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCtggtg                                                                                                                  >  1:560034/1‑141 (MQ=255)

                     gTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGGAAGCGGAGCAGGTCCtggtg                                                                                                                  >  1:630983/1‑141 (MQ=255)

                       gCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                                >  1:326238/1‑141 (MQ=255)

                       gCAACTGGCCAAAATGCAATGAAACACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                                <  1:282530/141‑1 (MQ=255)

                        cAACTGGCCAAAAGGCGATGAGTCTCATTAAACGTTGATTAAATAAGCCAGAATTATTACTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAGCAGCTACCATAGTAATCGGAGCAGGTCCTGGTGGAt                                                                                                               <  1:869757/141‑1 (MQ=255)

                          aCTGGCCAAAATGCAATGAATCACATTAAACGTGTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAc                                                                                                             <  2:974332/141‑1 (MQ=255)

                           cTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACg                                                                                                            >  2:623291/1‑141 (MQ=255)

                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTCTTATTAATGGAGGGGTCAAACATGGTAGTGGGCGATTTGCTAATTATAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                        >  1:186130/1‑141 (MQ=255)

                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGCTCCGTTgc                                                                                                        >  2:543159/1‑141 (MQ=255)

                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                        >  2:672932/1‑141 (MQ=255)

                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                        >  2:718606/1‑141 (MQ=255)

                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                        >  2:818286/1‑141 (MQ=255)

                                    aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCaa                                                                                                   <  1:137239/141‑1 (MQ=255)

                                       cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACCTGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGGGGATACGTTGCAGCAATTc                                                                                                >  2:847493/1‑141 (MQ=255)

                                       cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                                >  1:776944/1‑141 (MQ=255)

                                       cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                                >  1:88557/1‑141 (MQ=255)

                                       cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                                <  1:553330/141‑1 (MQ=255)

                                             aTCACATTAAACGTTTATTAAATAATCCAGAACTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAg                                                                                          <  1:25379/141‑1 (MQ=255)

                                               cacaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                        <  1:773875/141‑1 (MQ=255)

                                                acaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTGCAGGAATTTGTGCAGGTc                                                                                       >  2:232278/1‑141 (MQ=255)

                                                   ttAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAAt                                                                                    <  1:393283/141‑1 (MQ=255)

                                                      aaCGTTTATGAAATAAGCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAg                                                                                 <  1:514917/141‑1 (MQ=255)

                                                        cGTTTATTAAATAATCCAGAATTATTATTAATGAAGAGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCACAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCCCAATTAGGa                                                                               >  2:650753/1‑141 (MQ=255)

                                                         gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                              <  2:776944/141‑1 (MQ=255)

                                                         gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                              <  2:88557/141‑1 (MQ=255)

                                                                  ataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAAGAAGGACCAAAAGTaa                                                                     >  2:153381/1‑141 (MQ=255)

                                                                  ataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCCCAATTAGGAGAAAAAGTaa                                                                     >  2:741816/1‑141 (MQ=255)

                                                                   taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGCACAAAAAGTAAc                                                                    >  1:211808/1‑141 (MQ=255)

                                                                   taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAACTGGGAAAAAAAGTaaa                                                                    >  2:772783/1‑140 (MQ=255)

                                                                      tCCAGCATTATTATTAATGCAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAAt                                                                 >  1:144122/1‑141 (MQ=255)

                                                                      tCCAGAATTATTGTTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTGTAGCAGTTCGTGCAGCTCAATGAGGACAAAAAGTAACAAt                                                                 >  1:144279/1‑141 (MQ=255)

                                                                       ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                >  1:510697/1‑141 (MQ=255)

                                                                         aGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGt                                                              <  1:146616/141‑1 (MQ=255)

                                                                          gAATTATTATTAATGGCGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTGGGACAAAAAGTAACAATCGtt                                                             >  1:246384/1‑141 (MQ=255)

                                                                            attattattAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTga                                                           >  2:565246/1‑141 (MQ=255)

                                                                               attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTCGGACAAAAAGTAACAATCGATGAGaa                                                        >  2:486632/1‑141 (MQ=255)

                                                                               attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaa                                                        <  1:707904/141‑1 (MQ=255)

                                                                               attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACAAAAGTAATCGGAGCAGGACCTGATGGATACGTTGAAGCAATTAGTGCAGCTCATATAGGACAAAAAGTTAAAACCGTTGAGaa                                                        >  2:564548/1‑141 (MQ=255)

                                                                               aatattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaa                                                        <  1:707912/139‑1 (MQ=255)

                                                                                       tGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATc                                                <  1:591272/141‑1 (MQ=255)

                                                                                        ggAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCt                                               >  1:3775/1‑141 (MQ=255)

                                                                                        ggAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGGTGAGCAAGGTAATCt                                               >  1:122245/1‑141 (MQ=255)

                                                                                          gggggTAAGACATGGGAGTTGGGGGGGGCCCAGGTGGAACAGATGCCATAGGAATCGGGGCAGGTCCTGGTGGATACGTGGCAGCAATGCGTGCAGCTCAATTAGGACACACCTCGTCAGTCGTTGAGAAAGGTAATCTtg                                             <  2:701141/140‑1 (MQ=255)

                                                                                            gggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggt                                           <  1:748667/141‑1 (MQ=255)

                                                                                             ggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggtg                                          >  2:658693/1‑141 (MQ=255)

                                                                                             ggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATAAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTgggtg                                          >  2:612307/1‑141 (MQ=255)

                                                                                              gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggtgg                                         >  1:65499/1‑141 (MQ=255)

                                                                                               tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAGGTAACAATCGTTGGGAAAGGTAATCTTggtggt                                        >  2:81188/1‑141 (MQ=255)

                                                                                               tAAAACAGGGTAGTTGGAGATTTCTCAAGTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggtggg                                        >  1:628579/1‑140 (MQ=255)

                                                                                                 aaaTATGGTAGTTGGAGATTTACCAATTGAAACAGATACTTGAGAAAGCGGAGGAGGTCCTGTTGGATACGTTACACAAATTGATGCAGCACAACCAGGACAAAAAGTAACAATCGTCGAGAAAGGAAACATAGGGGgtgt                                      >  2:423953/1‑141 (MQ=255)

                                                                                                  accATGGGAATTGGAGTTTGTCCAATTGAAAAAGATCCCATAGGAATCGTACCAGGTACTGGTGGACACCTTGCAGCAATTCGTGCAGCTTAATTAGGACAAAAAGTAACAACCGTTGACAAAGGTAATCTTGGTGGTGtt                                     <  2:367176/139‑1 (MQ=255)

                                                                                                  aaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGCTGAGAAAGGTAATCTTGGTGGTGtt                                     >  2:176980/1‑141 (MQ=255)

                                                                                                  aaCAGGGTAGTGGGAGGTTTCGCAAGTGAAAAAGATGCCATAGTGATCGTAGCAGGTCCTGGTGGATACGTTGCAGCTATTCGCGCAGCTCAATTAGGACAAAAAGTAACAATAGTTGAGAAAGGTAATCTTGGTGGTGtt                                     <  2:523833/141‑1 (MQ=255)

                                                                                                   aCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                    >  1:853417/1‑141 (MQ=255)

                                                                                                   aCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGttt                                    >  1:218580/1‑141 (MQ=255)

                                                                                                    cattctAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTg                                   <  1:120865/136‑1 (MQ=255)

                                                                                                    cATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAAGCTTGGTGGTGTTTg                                   >  2:700009/1‑141 (MQ=255)

                                                                                                         tAGTGGGACATTTCCCATTGGAAACGGGTACCATAGTAATCTGAGCAGGTCCTGGTGGATACGTAGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTTAGAAAGGTAATCTTGGTGGTGTTTGCTTac                              <  2:326238/141‑2 (MQ=255)

                                                                                                           gTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATATTGGTGGTGTTTGCTTAAAc                            >  1:184077/1‑141 (MQ=255)

                                                                                                                 gaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAggg                      >  2:937233/1‑140 (MQ=255)

                                                                                                                  aTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGag                     >  2:227015/1‑140 (MQ=255)

                                                                                                                          aTTGAAACAGGTACCATAGTAGTCGGAGCAGGTCCTGGTGGATACGTTTCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCt             <  2:630983/141‑1 (MQ=255)

                                                                                                                              aaaCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGCACTCGTGGGGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaa         >  1:24021/1‑141 (MQ=255)

                                                                                                                               aaCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTCCaaa        <  1:672932/141‑1 (MQ=255)

                                                                                                                               aaCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTAGTGATTTTTAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaa        <  1:718606/141‑1 (MQ=255)

                                                                                                                                aCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaaa       >  2:743421/1‑141 (MQ=255)

                                                                                                                                   gATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCa    >  1:322963/1‑141 (MQ=255)

                                                                                                                                    aTACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAt   >  1:699411/1‑141 (MQ=255)

                                                                                                                                    aTACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAt   >  2:970453/1‑141 (MQ=255)

                                                                                                                                     tACTATAGGAATCGGTGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAtt  <  1:623291/141‑1 (MQ=255)

TTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATT  >  USA300TCH1516_ALE/1105858‑1106131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A