Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F52 I1 R1	27	78.9	1851050	96.3%	1782561	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,362	G→A	H104Y (CAC→TAC)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,362	0	G	A	97.2%	220.0 / NA	73	H104Y (CAC→TAC)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (31/38); minor base T (1/1); total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCA  >  USA300TCH1516_ALE/1744225‑1744498

ttcaattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACAt                                                                                                                                       <  2:227021/141‑1 (MQ=255)

    attATAACTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                                                                   <  1:702465/141‑1 (MQ=255)

    attATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                                                                   <  2:664304/141‑1 (MQ=255)

     ttATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTa                                                                                                                                  <  1:153751/141‑1 (MQ=255)

     ttATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTa                                                                                                                                  >  1:65493/1‑141 (MQ=255)

      tataAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTAATGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaa                                                                                                                                 >  2:678619/1‑141 (MQ=255)

         aaaTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAAc                                                                                                                              <  1:277916/141‑1 (MQ=255)

          aaTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACa                                                                                                                             <  2:540316/141‑1 (MQ=255)

               aaTACCCACTACGATCCCGCCTAATTTTCCAACTAATTTTATTGCTGCCTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                                                                        <  1:534085/141‑1 (MQ=255)

                aTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTa                                                                                                                       >  1:240162/1‑141 (MQ=255)

                   ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcct                                                                                                                    >  1:573482/1‑140 (MQ=255)

                    ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCat                                                                                                                   >  2:374157/1‑141 (MQ=255)

                      actacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCatat                                                                                                                 >  1:49410/1‑141 (MQ=255)

                      actacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCatat                                                                                                                 >  2:290844/1‑141 (MQ=255)

                         acGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                                                                              <  2:65493/141‑1 (MQ=255)

                           gATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAg                                                                                                            <  2:708261/141‑1 (MQ=255)

                            aTACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAgg                                                                                                           <  2:624833/141‑1 (MQ=255)

                             tACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGt                                                                                                          <  2:33597/141‑1 (MQ=255)

                                cGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACAACCGGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCTTTTTCTAGGTCAt                                                                                                       >  1:221731/1‑141 (MQ=255)

                                    tAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTTTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGATCATACTc                                                                                                   >  1:112025/1‑141 (MQ=255)

                                         tttCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAAc                                                                                              >  1:380546/1‑141 (MQ=255)

                                           tCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAACTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGa                                                                                            >  2:609133/1‑141 (MQ=255)

                                                    tttATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATGTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCa                                                                                   <  1:657734/141‑1 (MQ=255)

                                                      tATTGCAGCTTCAATCGTACCACCAATAGCTAATAAATCATCTGTAATTAACACACGTTTCCCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACg                                                                                 >  2:181867/1‑141 (MQ=255)

                                                          gctgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTTCATTGTTAAAACATTTGTAACATATTCTAGGTCATACTCATACAGAATGACTTAAAGAGGt                                                                             >  2:730483/1‑141 (MQ=255)

                                                            tgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTac                                                                           >  2:380878/1‑140 (MQ=255)

                                                            tgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTaa                                                                           >  2:504893/1‑141 (MQ=255)

                                                            tgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACATGGTTTAATTGCATCTTTGCACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATACGGAATAACTTCACGAGGTag                                                                           >  2:668046/1‑140 (MQ=255)

                                                            tgctTCAATCGAACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGAACCGGTTTACTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTat                                                                           >  2:619937/1‑140 (MQ=255)

                                                                    tCGTACCGCCTGTAGCTAATAAATCATTTGTCATTAACACACGTTGACCTGGTTTAATTGTATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGTTCATATTCATACCGAATGTCTTCACGAGGTAATTTCCctt                                                                   <  2:807621/141‑1 (MQ=255)

                                                                    tCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAGGACTTCACGAGGTAATTTCCctt                                                                   <  2:743528/141‑1 (MQ=255)

                                                                     cGTACCACCAGTAGATAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAATATATATTTACAAGGGTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCcttc                                                                  >  1:481751/1‑141 (MQ=255)

                                                                         ccaccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt                                                              <  2:893761/141‑1 (MQ=255)

                                                                         ccaccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt                                                              <  1:108376/141‑1 (MQ=255)

                                                                         ccaccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt                                                              <  1:846297/141‑1 (MQ=255)

                                                                           accaGTAGCTAATAAAGCATCTGTAATGAACACACGTTGACCTGGTTTAATTGGATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCt                                                            <  2:853564/141‑1 (MQ=255)

                                                                            ccaGTCGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCAGCTTTGTACATTGTTAAAACATTTGTACCATATTATAGGTAATCCTCATAACGAATGACTCCACGAGATAATATCCCTTCTTGTCTa                                                           >  2:692845/1‑141 (MQ=255)

                                                                            ccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTa                                                           >  2:613052/1‑141 (MQ=255)

                                                                                     aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCa                                                  <  1:608976/141‑1 (MQ=255)

                                                                                        aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                               <  1:840519/141‑1 (MQ=255)

                                                                                         aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGc                                              >  2:289196/1‑141 (MQ=255)

                                                                                         aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGGGCAAAGc                                              >  2:656928/1‑141 (MQ=255)

                                                                                           tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                            <  2:275974/141‑1 (MQ=255)

                                                                                           tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                            <  1:520244/141‑1 (MQ=255)

                                                                                           tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                            <  2:50023/141‑1 (MQ=255)

                                                                                           tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                            <  2:806835/141‑1 (MQ=255)

                                                                                             atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAt                                          >  1:545995/1‑141 (MQ=255)

                                                                                             atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAt                                          >  1:265426/1‑141 (MQ=255)

                                                                                             atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAt                                          <  1:139358/141‑1 (MQ=255)

                                                                                                  tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCTATTGACTGCTAACAGGTGCAAAGCCAATCCCCa                                     <  1:914236/141‑1 (MQ=255)

                                                                                                  tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCa                                     >  1:112902/1‑141 (MQ=255)

                                                                                                     ttAACACACGTTGACCTGGTTTAATTGCATCTTTTTACATAGATCCCCCGTTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTg                                  <  1:434095/141‑1 (MQ=255)

                                                                                                      tAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGa                                 <  2:847906/141‑1 (MQ=255)

                                                                                                      tAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGa                                 <  1:819726/141‑1 (MQ=255)

                                                                                                      tAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGa                                 <  1:718342/141‑1 (MQ=255)

                                                                                                        acatacGTTGACCTGGTTTGATTGCATGTTTGTACATTGTGAAAACATTTGGACCATATTCGAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                               <  1:874815/141‑1 (MQ=255)

                                                                                                        acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                               <  1:284094/141‑1 (MQ=255)

                                                                                                        acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                               <  1:609133/141‑1 (MQ=255)

                                                                                                          acacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATaa                             <  2:90035/141‑1 (MQ=255)

                                                                                                              ggtGACCTGGTTCAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGTTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTa                         <  1:800933/139‑1 (MQ=255)

                                                                                                               ttGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                        <  1:64661/141‑1 (MQ=255)

                                                                                                               gtcacgtGGTTTCATTGCATCTTTGTCCATTGTTAACACATTTTCACCATCTTCTAGGTCATCCTCATAACGAATGACTTCCCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                        <  1:205369/135‑1 (MQ=255)

                                                                                                                tGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACa                       >  1:192127/1‑141 (MQ=255)

                                                                                                                    cTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACCGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGAc                   >  1:736486/1‑141 (MQ=255)

                                                                                                                    cTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGAc                   >  1:736472/1‑141 (MQ=255)

                                                                                                                    cTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGAc                   >  1:828338/1‑141 (MQ=255)

                                                                                                                      ggTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATAGTCTAGGTCATACTCATAACGAATGACTTCACGAAGTAATTACTCTTCTTTTCTAACAGGTGCCAAGCCAACCCCCATTGAATAAGATACAGGACac                 >  2:506870/1‑140 (MQ=255)

                                                                                                                        tttAATCGCATCCTTGCACATTGTTCCAACATTTGTACCATATTCTAGGTCCTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGcc               <  1:191412/141‑1 (MQ=255)

                                                                                                                           aaTTGCATCTTTGTTCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTTCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAt            <  2:311842/141‑1 (MQ=255)

                                                                                                                           aaTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAt            <  1:802029/141‑1 (MQ=255)

                                                                                                                                cATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTACCCGGTGCAGAGGCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaa       >  2:455809/1‑141 (MQ=255)

                                                                                                                                cATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACCAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCTCATTGAATAAGCTACAGGACAGCCAATGATaa       >  1:615157/1‑141 (MQ=255)

                                                                                                                                     ttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCa  <  1:374157/141‑1 (MQ=255)

TTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCA  >  USA300TCH1516_ALE/1744225‑1744498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A