Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F67 I0 R1 44 83.9 1894234 96.6% 1829830 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,373,506 A→T 15.7% N90K (AAT→AAA)  pbuG ← Guanine/hypoxanthine permease PbuG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,373,5060AT15.7% 143.1 / 24.6 65N90K (AAT→AAA) pbuGGuanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base A (36/18);  new base T (3/7);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 3.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-02

TAAGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAAC  >  USA300TCH1516_ALE/2373366‑2373646
                                                                                                                                             |                                                                                                                                             
tAAGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCa                                                                                                                                               <  1:531348/141‑1 (MQ=255)
    gAATTGCATTAATAATAACTTCCCTAAGCCCGGGGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTa                                                                                                                                           >  1:455479/1‑141 (MQ=255)
           aTTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTACTCCCA‑t                                                                                                                                   >  2:739871/1‑141 (MQ=255)
               ataataaCTTCCCTAAACCCTATGACGGTTAATATCGCAAAGAATATACCTGAAAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCt                                                                                                                               <  1:511443/141‑1 (MQ=255)
                 aataaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTgg                                                                                                                             <  1:816272/141‑1 (MQ=255)
                 aataaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTgg                                                                                                                             <  2:10533/141‑1 (MQ=255)
                  ataaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAACACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGt                                                                                                                            >  1:835051/1‑141 (MQ=255)
                   taaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTg                                                                                                                           >  1:98723/1‑141 (MQ=255)
                   taaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTg                                                                                                                           <  1:461210/141‑1 (MQ=255)
                    aaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAAAATGCATTTAATCCAA‑TACCTGGTGc                                                                                                                          >  2:277026/1‑141 (MQ=255)
                     aCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCt                                                                                                                         >  2:926441/1‑141 (MQ=255)
                      cTTCCCTAAACCCTGTGACGGTTAATATCGCATAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CACGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTa                                                                                                                        <  1:630810/141‑1 (MQ=255)
                            tAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGCCTCCCTCAT‑GTGTACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCaa                                                                                                                  >  1:448083/1‑141 (MQ=255)
                            tAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGTTTAACACAACAGTAAATGCAAAGAATGCTTTTAAGCCCA‑TACCTGGTGCTAACGCaa                                                                                                                  <  2:269877/141‑1 (MQ=255)
                             aaaCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAAt                                                                                                                 <  1:104679/141‑1 (MQ=255)
                                cccTGCGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGC‑TTTAACGCCACAACCTGGTGCTAACGCAATTgg                                                                                                              <  1:456888/141‑1 (MQ=255)
                                 ccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACCTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGa                                                                                                             <  2:7943/141‑1 (MQ=255)
                                    gtgACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATAGGatat                                                                                                          >  2:937816/1‑141 (MQ=255)
                                    gtgACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACATGGTGCTAACGCAATTGTatat                                                                                                          >  1:186414/1‑141 (MQ=255)
                                    gtgACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACCCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTACTAACGCAATTGGatat                                                                                                          >  1:490161/1‑141 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAGTACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGAAATTTa                                                                                                       >  2:447048/1‑141 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACCACAGTAAATGCACAGAATGCATTTAATAGAA‑TAACTGGGGCTAACGCAATTGGATAtttc                                                                                                       >  2:150402/1‑140 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGGTATTTa                                                                                                       >  2:161501/1‑141 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATAttca                                                                                                       >  1:331576/1‑139 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTa                                                                                                       >  1:865261/1‑141 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGGGCTAACGCAATTGGATAtttg                                                                                                       >  1:419867/1‑140 (MQ=255)
                                        cGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAg                                                                                                      >  1:920449/1‑141 (MQ=255)
                                              atatCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCGATTGGATATTTAGCTATTa                                                                                                <  1:562849/141‑1 (MQ=255)
                                                 tCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGcc                                                                                             <  2:98723/141‑1 (MQ=255)
                                                   gCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGcccc                                                                                           <  1:716926/141‑1 (MQ=255)
                                                   ccAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGcccc                                                                                           <  1:23736/140‑1 (MQ=255)
                                                    cAAAGAATCTACCTGAGCATAAAACGCCTGCTAAACCAACTTGCCACGCAATACC‑CATGGTTAACACAACAGTCAACGCCAAGAATGCACTTACTCCCA‑TACCTGGTGCTAACGCACTTGCCCACTTAGCTATCCGccccc                                                                                          >  2:864575/1‑140 (MQ=255)
                                                    cAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCa                                                                                          <  2:455479/141‑1 (MQ=255)
                                                       aGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTGGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGa                                                                                       <  1:779826/141‑1 (MQ=255)
                                                           tataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAg                                                                                   <  2:187452/141‑1 (MQ=255)
                                                              aCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑AATGGTTAACACAACAGTAAATGCAAAGAATGCAGTTAATCCAA‑TACCTGGTGCTAACGCAAATGGATAGTTAGCTATTAGCCCCATGAATAGCgg                                                                                >  2:844930/1‑140 (MQ=255)
                                                                   agaATAAACCGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTa                                                                           <  2:72705/141‑1 (MQ=255)
                                                                     aaTAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACa                                                                         >  1:577079/1‑141 (MQ=255)
                                                                                 gTTAAACCAACTTGCCAAGGAATACC‑CATGGTTCACACCACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAAGTCCATCGGCGCGTGATGTAGGAGCCCCATGAATAGCGAGCATACAAATGCTGCTaag                                                             >  1:306826/1‑140 (MQ=255)
                                                                                    aaaCCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGATGCTAATGCa                                                          >  1:633476/1‑141 (MQ=255)
                                                                                        cAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGAGATTTAGCTATTAGCCCCATGAATAGCGAGCGTACAAATGCCGCTAATGCGGTcc                                                      >  2:330861/1‑140 (MQ=255)
                                                                                         aaCTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGc                                                     <  2:362337/141‑1 (MQ=255)
                                                                                           cctGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTa                                                   <  1:855822/139‑1 (MQ=255)
                                                                                             ggCCAAGGAATACC‑CAGGGTTAACACAACGGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACa                                                 <  1:864575/140‑1 (MQ=255)
                                                                                              gCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACaa                                                >  1:492129/1‑141 (MQ=255)
                                                                                                 aaGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACaaaaa                                             >  2:819116/1‑141 (MQ=255)
                                                                                                  atgAATACC‑CATGTTTAACACAACATTAAATGCAAAGAATGCATTTAACCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCGATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAAt                                            <  2:539654/139‑1 (MQ=255)
                                                                                                  aGGAATACC‑CATGGCTAACACGACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATGTTTAGCTATTAGACCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAAt                                            >  2:646544/1‑141 (MQ=255)
                                                                                                        aCC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCAcc                                      >  1:752701/1‑141 (MQ=255)
                                                                                                         cc‑cATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTGTTTATTATCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCt                                     >  1:686564/1‑141 (MQ=255)
                                                                                                              tGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGt                                 <  2:308482/141‑1 (MQ=255)
                                                                                                              tGGTTAAAACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCGAACTTAATTGGATATTTAGCTATTCGCCCGATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGt                                 <  2:705206/141‑1 (MQ=255)
                                                                                                                    agacaacaGTCAATACAAGGAATGCATTTAATGTCA‑TAACTAATGGAACTGCATTTATTGAGTAAGTTGTTGGCGCCAGGAATAGCGAGACTACAAATGCTGCTAATGCAGTGGCTAAAAAAGGGGCCACGTGGTCAAttt                           >  2:429974/3‑141 (MQ=255)
                                                                                                                    acacAACAGTAAATGCAAAGAATGCTTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                           >  1:578818/1‑141 (MQ=255)
                                                                                                                    acacAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCCGTCGCTACAAAAATGGCACCTTGGTGCCttt                           >  1:843/1‑141 (MQ=255)
                                                                                                                    acacAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                           >  1:144763/1‑141 (MQ=255)
                                                                                                                    acacAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                           >  1:493949/1‑141 (MQ=255)
                                                                                                                     tacaacaGTAAATGCAAAGTATGCATTTAATCCCA‑TTCCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAtttt                          <  1:157232/140‑1 (MQ=255)
                                                                                                                     cacaACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAtttt                          <  2:114710/141‑1 (MQ=255)
                                                                                                                              aaaTGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtcttc                 <  2:733594/141‑1 (MQ=255)
                                                                                                                               aaTGGAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACCAAAATGGCACCATGGTCAATGTTCGAAtcttct                >  2:571315/1‑141 (MQ=255)
                                                                                                                               aaTGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTGCATTTTCATAtcttct                >  2:412281/1‑141 (MQ=255)
                                                                                                                               aaTGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtcttct                >  2:828842/1‑141 (MQ=255)
                                                                                                                               aaTGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAccttct                >  2:224222/1‑141 (MQ=255)
                                                                                                                                      aGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGc         >  1:146781/1‑141 (MQ=255)
                                                                                                                                           gCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTa    >  2:876030/1‑141 (MQ=255)
                                                                                                                                             aTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACACAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTacc  >  2:259595/1‑139 (MQ=255)
                                                                                                                                             aTTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCCCATTGGTCCCTTTTCCTATCTTCTGAAAAACCTTTAac  >  1:377954/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                             
TAAGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACC‑CATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCA‑TACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAAC  >  USA300TCH1516_ALE/2373366‑2373646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A