Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F121 I0 R1 45 69.8 1582072 96.2% 1521953 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,000,948 C→T 64.3% T11I (ACA→ATA)  spxA → Regulatory protein Spx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,000,9480CT64.3% 51.9 / 58.5 66T11I (ACA→ATA) spxARegulatory protein Spx
Reads supporting (aligned to +/- strand):  ref base C (14/9);  new base T (22/20);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

AATAAATAGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACTGATGAAA  >  USA300TCH1516_ALE/1000812‑1001082
                                                                                                                                         |                                                                                                                                      
aataaataGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTTTTGTTCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGATTGAGATGTATGGTAACATCATTTAATTCACCAAATTGCACATCt                                                                                                                                    >  2:1298/1‑141 (MQ=255)
   aaataGTAACGATTAAGAATTGTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGc                                                                                                                                 <  1:773101/141‑1 (MQ=255)
    aataGTAACGATTAAGACTTTTAGAAAAAAATGGTTTTTCTTGATCTATAACTAAATTTTAAGCCATGAATTTTATTCATTCAATT‑AGGAGATTGAGATGTATGGTAACATAATTTAGTTTCCTATGTTGCatatttagct                                                                                                                                >  2:740783/1‑135 (MQ=255)
      taGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGt                                                                                                                              >  1:506049/1‑141 (MQ=255)
       aGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTa                                                                                                                             >  2:657943/1‑141 (MQ=255)
       aGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTa                                                                                                                             >  1:570877/1‑141 (MQ=255)
        gTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTaa                                                                                                                            >  1:81620/1‑141 (MQ=255)
        gTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTaa                                                                                                                            >  1:746012/1‑141 (MQ=255)
           aCGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGc                                                                                                                         <  1:53240/141‑1 (MQ=255)
           aCGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATAACAAAAAATTAAGGTTATTCATTCCCCTCATCAGCCACA‑AGGGATGGAAACAGGATGTACTTCACCAAGTTGCACATCTTGCCGTAAAGc                                                                                                                         >  2:359875/1‑141 (MQ=255)
               ttAAGAATTTTAGAAAAAAATGTTTTTTTTTTTTTTTTTACTAAATTTTAGGCAATGAATTTTGTTTATTCTGAT‑AGGAGAGTGAGATGTATGTTTTTATAATTTAATTCACCATTTTGTATTTCTTGCAGTAAAgttaat                                                                                                                     >  2:727696/1‑136 (MQ=255)
                tAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAg                                                                                                                    >  1:414736/1‑141 (MQ=255)
                 aaGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGc                                                                                                                   >  2:286283/1‑141 (MQ=255)
                 aaGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGc                                                                                                                   >  2:602708/1‑141 (MQ=255)
                  aGAATTTTAGAGAAAAAGTTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGCGAGTGCGATGTATGGTAACATTAGTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCa                                                                                                                  <  2:70451/141‑1 (MQ=255)
                  aGAATTATAAAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCa                                                                                                                  <  2:674515/141‑1 (MQ=255)
                   gAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGGGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGTCGTAAAGCGAAAGCAt                                                                                                                 >  2:310037/1‑141 (MQ=255)
                   gAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCAt                                                                                                                 >  1:27570/1‑141 (MQ=255)
                      ttttAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGt                                                                                                              >  1:725071/1‑141 (MQ=255)
                      ttttAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGt                                                                                                              <  2:669535/141‑1 (MQ=255)
                           gAAAAAAATGTTTTGTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTAGTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACaa                                                                                                         >  2:328765/1‑141 (MQ=255)
                            aaaaaaaTGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAg                                                                                                        >  1:297650/1‑141 (MQ=255)
                                  aTGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATg                                                                                                  >  2:718352/1‑141 (MQ=255)
                                  aTGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTCCACCTTGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATg                                                                                                  <  2:759609/141‑1 (MQ=255)
                                  aTGTTTTTTCTTGATCTATAACTAAATCTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATg                                                                                                  <  1:666519/141‑1 (MQ=255)
                                    gTTTTTTCTTGATCTATAACTAAATTTTAAGCACGCACGTTCACTCTTGGAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGAc                                                                                                <  2:164042/141‑1 (MQ=255)
                                                      actgacttttAAGCAATGAAGTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGt                                                                              <  2:572577/135‑1 (MQ=255)
                                                      aCTAAATTTTAAGCAATGTATTTTATTCATCCCAAT‑AGGAGAGTGAGATGTATGGTAACATTAGTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGt                                                                              <  2:744180/141‑1 (MQ=255)
                                                      aCTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGt                                                                              <  2:251169/141‑1 (MQ=255)
                                                               tAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATAttttt                                                                     >  1:312299/1‑141 (MQ=255)
                                                                aaGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATAtttttt                                                                    <  1:443895/141‑1 (MQ=255)
                                                                   cAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTCTGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGCAAGCATGGTTACAAGAACATGACATTCCGTATACCGAGCGTAATATTTTTTCTg                                                                 <  1:512439/141‑1 (MQ=255)
                                                                   cAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTg                                                                 <  1:695987/141‑1 (MQ=255)
                                                                   cAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTg                                                                 <  1:146998/141‑1 (MQ=255)
                                                                    aaTGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGa                                                                <  1:324292/141‑1 (MQ=255)
                                                                     aTGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGaa                                                               >  1:138896/1‑141 (MQ=255)
                                                                        aaTTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACAt                                                            <  1:359875/141‑1 (MQ=255)
                                                                          ttttATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCCACAAGTTGCAAAACTTGCCATAAAGCGAAAGCATGGTTACCAGAAAATGACATTCCGTATACGAAACGAGATATTGTTTCTTAACAttt                                                          >  2:599817/1‑141 (MQ=255)
                                                                          ttttAGTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATGTACTCCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACAttt                                                          <  1:84639/141‑1 (MQ=255)
                                                                               ttcattcaAAG‑AAGAGAGTGACTTGATGGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACaa                                                     <  2:197254/141‑1 (MQ=255)
                                                                                 aatttaAAT‑CGGAGAGTTAGACTTATGGTAACATTATTTACTTAACACACACGCACATCTTCCCCTCACGCTCTCCACTTAATACTAAGACATGTCATACCGCATACGGAGCGTAATATGCCTTCTGAACATTTAACAAtt                                                   <  2:197728/140‑1 (MQ=255)
                                                                                  gttcaAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATtg                                                  >  2:172816/2‑141 (MQ=255)
                                                                                   ttcaAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATtga                                                 <  2:570877/141‑1 (MQ=255)
                                                                                   ttcaAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATtga                                                 >  1:667332/1‑141 (MQ=255)
                                                                                    tcaAAT‑AGGATAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATtgat                                                <  2:381557/141‑1 (MQ=255)
                                                                                            ggAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACCATTGATGAAATTa                                         >  1:725978/1‑141 (MQ=255)
                                                                                            ggAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTa                                         >  2:161927/1‑141 (MQ=255)
                                                                                            ggAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTa                                         >  2:82752/1‑141 (MQ=255)
                                                                                             gagaGTGAGATGTATGATAACATTATTTAATTCACCATGCTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTaa                                        <  2:201253/141‑1 (MQ=255)
                                                                                               gagTGAGATGTATGGTAACATTATTAGCTTCACCAAGTTGCATATTTTGCCGGAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGc                                      <  2:320565/141‑1 (MQ=255)
                                                                                                agTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATCTTTTTTCTGAACATTTAACAATTGATGAAATTAAGCa                                     >  1:144661/1‑141 (MQ=255)
                                                                                                agTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCa                                     <  1:630165/141‑1 (MQ=255)
                                                                                                agTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCa                                     <  1:718352/141‑1 (MQ=255)
                                                                                                     gacgTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGGAATTAAGCAAatat                                >  2:597592/4‑141 (MQ=255)
                                                                                                     gaTGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAatat                                <  1:435588/141‑1 (MQ=255)
                                                                                                      aTGTATGGTAACATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATAtt                               >  1:606397/1‑141 (MQ=255)
                                                                                                                 cATTATTTACTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTg                    >  1:327888/1‑141 (MQ=255)
                                                                                                                     attTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGa                >  2:491693/1‑141 (MQ=255)
                                                                                                                          cTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTCAGCAAATATTAAAAATGAATGAAGACGGTa           >  2:503417/1‑141 (MQ=255)
                                                                                                                          cTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTa           >  2:306160/1‑141 (MQ=255)
                                                                                                                          cTTCACCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGAAGGTa           >  2:410275/1‑141 (MQ=255)
                                                                                                                            tCACCAAGTTGCACATTTTGCCGTAAAGCGAAAGCATGGTTACAAGAACAGGACATTCCGTATACGGAGCGTAATATTCTTTCTGAACATTTAACAATTGAGGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACt         <  1:544240/141‑1 (MQ=255)
                                                                                                                            tCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACt         >  2:251060/1‑141 (MQ=255)
                                                                                                                              aCCAAGTTGCATATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACtga       >  2:758811/1‑141 (MQ=255)
                                                                                                                              aCCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACtga       >  2:84023/1‑141 (MQ=255)
                                                                                                                                aaaGTTGCATATTTTGCCGAAAATCGAAAGCATGGTTACAACAACATCACATTCCGTATACGGAGCGTAATATTTTTCCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACtgatg     <  1:20963/140‑1 (MQ=255)
                                                                                                                                   gTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGACGACGGTACTGATGaaa  >  2:492160/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AATAAATAGTAACGATTAAGAATTTTAGAAAAAAATGTTTTTTCTTGATCTATAACTAAATTTTAAGCAATGAATTTTATTCATTCAAAT‑AGGAGAGTGAGATGTATGGTAACATTATTTACTTCACCAAGTTGCACATCTTGCCGTAAAGCGAAAGCATGGTTACAAGAACATGACATTCCGTATACGGAGCGTAATATTTTTTCTGAACATTTAACAATTGATGAAATTAAGCAAATATTAAAAATGACTGAAGACGGTACTGATGAAA  >  USA300TCH1516_ALE/1000812‑1001082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A