Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F43 I1 R1	11	20.8	477872	94.5%	451589	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	64.7 / NA	23	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (10/13); total (10/13)

CACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATG  >  USA300TCH1516_ALE/1646058‑1646308

cACTTGCATCACCCTTTGGTACTGCACCTCTGTGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                <  1:100722/141‑1 (MQ=255)

cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCACTCATCCCTCCTTTTacaa                                                                                                                >  2:33513/1‑141 (MQ=255)

       aacaacATGTGGTAATGCACCTCTATGACCAGATGCTACAATAGTATCGAATGATGTTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTATAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                         <  2:26813/141‑1 (MQ=255)

        acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                        >  2:186194/1‑141 (MQ=255)

            cATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                                                    >  1:110117/1‑141 (MQ=255)

                  gTAATGCAACTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTTCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                              <  2:202735/141‑1 (MQ=255)

                                        tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                        <  2:175842/141‑1 (MQ=255)

                                               aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                 >  1:220481/1‑141 (MQ=255)

                                                           gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                     <  1:205172/141‑1 (MQ=255)

                                                                     cTGCTCCTAATTCTAGCATTTTGTTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAg                                           <  2:124851/141‑1 (MQ=255)

                                                                        cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                        <  2:110117/141‑1 (MQ=255)

                                                                               ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTCTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGGTGCTTTTTGAATTGAAGCACTTTCGTCAGCGTCTTTGAc                                 >  1:138406/1‑141 (MQ=255)

                                                                                  tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                              <  1:186194/141‑1 (MQ=255)

                                                                                    gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                            >  1:108772/1‑141 (MQ=255)

                                                                                          tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                      <  1:210606/141‑1 (MQ=255)

                                                                                            cTTTCTGATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                    <  2:160970/141‑1 (MQ=255)

                                                                                                      ttGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAATTATATTCATATGTTTTATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTAAGCATCTTTGACTTCTCTAATCTTATCTACAGTAt          >  2:109086/1‑141 (MQ=255)

                                                                                                      ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAACATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAATCAATTTCGTCAGCATCTTTGTCGTCTCTATTTTTCTCTTCAGTAt          >  2:12712/1‑141 (MQ=255)

                                                                                                      ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACCATATTAGCTGCTTTTTGAATTAAAGAAATTTCTTCAGCATCTTTAACTTCTTTAATTTTATCTACAGTAt          >  2:191943/1‑141 (MQ=255)

                                                                                                      ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACTTCTCTAATTTTATCTACAGTAt          >  2:201734/1‑141 (MQ=255)

                                                                                                           tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACCAAAATAATATTTTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa     <  1:101771/141‑1 (MQ=255)

                                                                                                              aaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg  <  1:44847/141‑1 (MQ=255)

                                                                                                              aaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg  <  1:168840/141‑1 (MQ=255)

CACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATG  >  USA300TCH1516_ALE/1646058‑1646308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A