Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F74 I0 R1 29 68.9 1565664 94.4% 1477986 140.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT)  femA_3 → Aminoacyltransferase FemA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,532,2980AT100.0% 236.7 / NA 73T15T (ACA→ACT) femA_3Aminoacyltransferase FemA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/47);  total (26/47)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC  >  USA300TCH1516_ALE/2532164‑2532434
                                                                                                                                      |                                                                                                                                         
gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                     <  2:525645/141‑1 (MQ=255)
gtATTTGTAATAAGTTTAAGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATGATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                     <  2:310715/141‑1 (MQ=255)
 tataTGTAATAAGATTTAGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                    <  2:227456/141‑1 (MQ=255)
 tATTTGTAATTAGTGTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                    <  2:32778/141‑1 (MQ=255)
   tttGTAATCATTATTTGAAAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAt                                                                                                                                  <  2:270854/141‑1 (MQ=255)
     tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAACATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                                <  1:620783/141‑1 (MQ=255)
     tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                                <  1:621215/141‑1 (MQ=255)
       tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTAAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                              >  1:370359/1‑141 (MQ=255)
            aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGTAGGGGTTCAATGCGGTTACTACAAAGCATTTTTCACAt                                                                                                                         >  2:625140/1‑141 (MQ=255)
            aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGAGGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                         <  1:660744/141‑1 (MQ=255)
            aGTTGTTGATAAAATAAGAAAATGGGATTATTAATAAATTCAAGAATTATAGTTCAATTCAGAGAGGGAGGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                         <  2:146565/141‑1 (MQ=255)
             gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATAATAGATCAATTCAGGTGGGGATGGATTAAAATAAACTTTATAACATTGACTTCTGATAGGTTAAATGCTTTTACTACAAAACATCTTTcaaact                                                                                                                        >  2:10566/1‑137 (MQ=255)
              tttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa                                                                                                                       >  1:84405/1‑141 (MQ=255)
                tttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                                     >  1:234652/1‑141 (MQ=255)
                  agATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                   <  2:727485/140‑1 (MQ=255)
                   gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                                  >  1:80894/1‑141 (MQ=255)
                   aataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                                  <  2:167497/140‑1 (MQ=255)
                        aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                             <  1:588545/141‑1 (MQ=255)
                          taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑c                                                                                                          >  1:552129/1‑141 (MQ=255)
                          taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑c                                                                                                          <  1:609351/141‑1 (MQ=255)
                           aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑Ct                                                                                                         <  2:19664/141‑1 (MQ=255)
                           aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑Ct                                                                                                         <  1:314096/141‑1 (MQ=255)
                           aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑Ct                                                                                                         <  1:393290/141‑1 (MQ=255)
                           aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑Ct                                                                                                         >  1:611998/1‑141 (MQ=255)
                              aaaaTAGCATTATTAATAAATTCACAAAGTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTAtt                                                                                                      >  2:57872/1‑141 (MQ=255)
                              aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTAtt                                                                                                      >  1:235925/1‑141 (MQ=255)
                               aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTCTTc                                                                                                     >  1:287589/1‑141 (MQ=255)
                                aaTAGCATTGTAAATAAAGTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCa                                                                                                    <  2:272206/141‑1 (MQ=255)
                                aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCa                                                                                                    <  2:660212/141‑1 (MQ=255)
                                aaTAGCATTATTAATAAATTCAAAAATTATAGTTAAATCTATAGAGGGATGGGATGAAATGAAGTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCa                                                                                                    <  2:588403/141‑1 (MQ=255)
                                         tttaAAAGATTCAAAAAGTATAGTTCAAATAAGAGAGGGATGGGATAAAATGAATTTTGTAACGTTGATTTCGGATGGGTTCAAGGCTGTGACTACAAAGCATTATTCACATTACACACAATCAG‑CTATTCATTACAATCa                                                                                           <  2:13252/140‑1 (MQ=255)
                                         attaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCa                                                                                           <  1:594889/141‑1 (MQ=255)
                                                aaTTCAAAAAGTATAGTTGAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGtt                                                                                    <  2:269745/141‑1 (MQ=255)
                                                   tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGAt                                                                                 >  1:144692/1‑141 (MQ=255)
                                                     aaaaaaTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGAttt                                                                               <  1:141408/141‑1 (MQ=255)
                                                       aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTaa                                                                             <  1:229694/141‑1 (MQ=255)
                                                        aaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTATACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTaaa                                                                            <  1:751500/141‑1 (MQ=255)
                                                        aaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTaaa                                                                            <  2:445487/141‑1 (MQ=255)
                                                            ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                        <  1:553468/141‑1 (MQ=255)
                                                            ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                        >  1:138218/1‑141 (MQ=255)
                                                                ttCAATTCAGAGAGGGATGGGATAGAATGAACTTTGTAACGTTGAGTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                    <  2:574226/141‑1 (MQ=255)
                                                                ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                    <  2:363498/141‑1 (MQ=255)
                                                                   aaTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc                                                                 <  1:312405/141‑1 (MQ=255)
                                                                    aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCa                                                                <  2:377273/141‑1 (MQ=255)
                                                                       aagagggGGATGGGATAAAATGATCTTTGTAACGTTGACTTCGGATGAGTTGAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑TTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                             <  2:574626/140‑1 (MQ=255)
                                                                        agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAACAGGCGATGTGCACCtt                                                            >  1:621269/1‑141 (MQ=255)
                                                                             gggatgggatAAGATGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTAACATGACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                       <  2:8091/141‑1 (MQ=255)
                                                                                atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  2:186031/141‑1 (MQ=255)
                                                                                ataggatAAAATGAAGTTTGTAATGTTGAATTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTTTTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  2:659170/138‑1 (MQ=255)
                                                                                     atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg                                               <  1:116538/141‑1 (MQ=255)
                                                                                       aaaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTGAAAGGCGATGTGCATCTTGGAGGGGTGAAGGAt                                             >  1:114246/1‑141 (MQ=255)
                                                                                       aaaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATAAG‑CTATTCATTACACTCATAGAGTTGATTTAAAAAACCATGTGCATTTTGTAAGTGTTAAAGaa                                             >  2:429862/1‑140 (MQ=255)
                                                                                        ataTGAACTTTGTACCTTTGCCGTCTGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAA‑CAGCCTATTCATGACAATAATATAGGTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                            <  2:95086/139‑1 (MQ=255)
                                                                                        aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                            <  2:235925/141‑1 (MQ=255)
                                                                                        aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                            <  2:646195/141‑1 (MQ=255)
                                                                                               cTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATgg                                     <  1:297958/141‑1 (MQ=255)
                                                                                                tttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGt                                    <  2:510401/141‑1 (MQ=255)
                                                                                                  tGTAACGTTGACTTCGGATGCGTTCACTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCGTAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                  <  2:125539/141‑1 (MQ=255)
                                                                                                  tGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAACTGCGTTGAGACAGTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                  <  2:756938/141‑1 (MQ=255)
                                                                                                    tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                                >  1:453202/1‑141 (MQ=255)
                                                                                                    tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                                >  1:141451/1‑141 (MQ=255)
                                                                                                     aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATATTGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGt                               >  2:634735/1‑141 (MQ=255)
                                                                                                     aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTAAAga                               >  2:631164/1‑140 (MQ=255)
                                                                                                      aCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg                              >  1:10984/1‑141 (MQ=255)
                                                                                                              ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                      <  2:80894/141‑1 (MQ=255)
                                                                                                                cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                    >  1:506338/1‑141 (MQ=255)
                                                                                                                cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                    >  1:148707/1‑141 (MQ=255)
                                                                                                                cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATATTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                    >  2:739037/1‑141 (MQ=255)
                                                                                                                  gATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGc                  >  1:476203/1‑141 (MQ=255)
                                                                                                                         tccacgCGTTTACTACAAAGCATTCTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa           <  1:81939/136‑1 (MQ=255)
                                                                                                                                gTTTACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgc    >  1:150369/1‑141 (MQ=255)
                                                                                                                                 tttACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca   <  1:57872/141‑1 (MQ=255)
                                                                                                                                  ttACTACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac  <  2:552129/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAG‑CTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC  >  USA300TCH1516_ALE/2532164‑2532434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A