Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,178,810 G→C P282P (CCC→CCG)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,178,8100GC100.0% 490.7 / NA 168P282P (CCC→CCG) gatZD‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (168/0);  total (168/0)

Only 100 of 168 total aligned reads displayed.

TGCAAATATCGCCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAG  >  W3110S.gb/2178774‑2178834
                                    |                        
cgCAAATATCGCCTCGCGTAACGCTAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1273079/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGa                       >  1:595960/2‑40 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCaaa           >  1:1332485/2‑51 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAaaat         >  1:754288/2‑54 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAAttc       >  1:479735/2‑55 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAAtt        >  1:672315/2‑55 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:511952/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:659245/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:644647/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:627109/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:561687/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:551423/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:550344/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:525117/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:521083/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:686828/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:497995/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:487833/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:460948/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:455720/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:445624/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:429549/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:402856/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:358616/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:344259/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:333544/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:326652/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:296703/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:843539/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:987696/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:968526/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:956297/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:954175/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:935555/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:921220/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:918943/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:896475/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:882444/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:877692/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:869551/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:668645/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:835721/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:808534/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:789309/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:786221/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:770963/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:738735/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:723102/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:712246/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:711929/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:278625/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1165629/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1340907/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1335886/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1303104/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1290665/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1272069/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1251196/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1250261/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:123873/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1212880/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1201326/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1179365/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1357567/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1163915/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1149161/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1139331/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1130199/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1119928/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1116328/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1102613/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1088217/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1083053/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1066422/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1047519/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1505396/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1004129/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:2767/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:258378/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:221832/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:215735/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:202202/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:171787/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1609818/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1569713/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1546127/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1540112/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:2877/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1483770/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1475471/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:146739/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1456931/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:14404/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1428820/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1427076/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1421372/2‑56 (MQ=255)
cgCAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1411654/2‑56 (MQ=255)
cgCAAATATCGACTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAAtt        >  1:1464286/2‑55 (MQ=255)
cacAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1136720/3‑56 (MQ=255)
cacAAATATCGCCTCGCGTAACGCAAAGGTTAATGCCGGACCGACTTTCAAAATTg       >  1:1391522/3‑56 (MQ=255)
                                    |                        
TGCAAATATCGCCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAG  >  W3110S.gb/2178774‑2178834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A