Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
821,826 |
A→T |
38.6% |
intergenic (‑363/+91) |
ldrA ← / ← ldrC |
toxic polypeptide, small/toxic polypeptide, small |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 821,826 | 0 | A | T | 38.6%
| 32.5
/ 48.2
| 44 | intergenic (‑363/+91) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base A (9/18); new base T (7/10); total (16/28) |
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCC > minE/821760‑821887
|
acAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGAt < 1:2122423/69‑1 (MQ=255)
aGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa < 1:105634/71‑1 (MQ=255)
gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:1774006/1‑70 (MQ=255)
tttCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaa < 1:3172004/71‑1 (MQ=255)
ttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa > 1:773758/1‑65 (MQ=255)
tCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa > 1:278430/1‑70 (MQ=255)
aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAAt < 1:3394965/71‑1 (MQ=255)
tGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATg < 1:662431/71‑1 (MQ=255)
gAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATg < 1:2399610/70‑1 (MQ=255)
gTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGcc < 1:3825580/71‑1 (MQ=255)
ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGc < 1:1552748/70‑1 (MQ=255)
ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2069879/71‑1 (MQ=255)
ctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1268934/69‑1 (MQ=255)
ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATaaa > 1:3393867/1‑53 (MQ=255)
ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCg > 1:807849/1‑67 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:668563/1‑71 (MQ=255)
atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt < 1:2425254/71‑1 (MQ=255)
atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt > 1:535585/1‑71 (MQ=255)
tgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt > 1:1029920/1‑70 (MQ=255)
atgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:2162208/66‑1 (MQ=255)
gCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2080475/60‑1 (MQ=255)
ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaaaa < 1:3702351/48‑1 (MQ=255)
ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc > 1:3201175/1‑71 (MQ=255)
cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCg < 1:209352/71‑1 (MQ=255)
gCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCgg > 1:55312/1‑70 (MQ=255)
cTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCg < 1:3689502/68‑1 (MQ=255)
cGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGAc < 1:3634067/37‑1 (MQ=255)
gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCg < 1:3682159/47‑1 (MQ=255)
gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCg < 1:1124784/47‑1 (MQ=255)
gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAAt < 1:2931438/40‑1 (MQ=255)
gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAAt < 1:2992327/40‑1 (MQ=255)
ttGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc < 1:1627388/71‑1 (MQ=255)
ctGCCAATATTTACTTGTGGAAGTGATAAAGACaaaa < 1:1712164/36‑1 (MQ=39)
gCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc > 1:2977712/1‑40 (MQ=255)
cAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgc > 1:3890915/1‑71 (MQ=255)
cAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt < 1:2076553/48‑1 (MQ=255)
atatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:3503344/40‑1 (MQ=255)
atatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcg > 1:2478154/1‑70 (MQ=255)
tatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc > 1:165946/1‑42 (MQ=255)
atTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa > 1:199331/1‑70 (MQ=255)
aCTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTg > 1:2829459/1‑71 (MQ=255)
gtgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGcc < 1:864509/69‑1 (MQ=255)
tgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTAc < 1:535924/38‑1 (MQ=255)
aaGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGcc < 1:29224/45‑1 (MQ=255)
agaTAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc > 1:848335/1‑38 (MQ=255)
|
GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCC > minE/821760‑821887
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
