Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,826,261 |
C→A |
20.6% |
A21A (GCG→GCT) |
hydN ← |
formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,826,261 | 0 | C | A | 20.6%
| 208.1
/ 27.9
| 82 | A21A (GCG→GCT) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base C (37/28); new base A (13/4); total (50/32) |
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 |
GATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTC > minE/1826192‑1826330
|
gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCAc < 1:1998606/70‑1 (MQ=255)
cgcgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTAc < 1:1741691/71‑1 (MQ=255)
ggTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt > 1:2227259/1‑70 (MQ=255)
ggTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt > 1:2232043/1‑70 (MQ=255)
ggTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt > 1:1351630/1‑70 (MQ=255)
tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTcac < 1:1640991/71‑1 (MQ=255)
tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTca > 1:1351575/1‑70 (MQ=255)
tAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTcac < 1:3690796/71‑1 (MQ=255)
aaaaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTca > 1:3104207/1‑69 (MQ=255)
aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAgg > 1:209274/1‑71 (MQ=255)
aaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:2554580/1‑70 (MQ=255)
aaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:1732410/1‑70 (MQ=255)
tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACgg < 1:3952329/71‑1 (MQ=255)
cGGGGGCAGCGATGCACAGTCCTGATTTTCCAGATGAGAAACCGCGCACGCTACTTc < 1:3913386/57‑1 (MQ=38)
gggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCaa > 1:892809/1‑68 (MQ=255)
gggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCa < 1:3188542/71‑1 (MQ=255)
tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATa < 1:1548548/71‑1 (MQ=255)
tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATa < 1:1551969/71‑1 (MQ=255)
aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACa < 1:3711314/71‑1 (MQ=255)
aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGTCAACCAATAc < 1:257914/70‑1 (MQ=255)
aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa < 1:2733904/71‑1 (MQ=255)
aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa > 1:456722/1‑71 (MQ=255)
aGCGATGCAAAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa > 1:3773237/1‑71 (MQ=255)
cGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACAtt > 1:1524184/1‑71 (MQ=255)
aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGTAACCAATACATTTa > 1:1004968/1‑71 (MQ=255)
tGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc > 1:3501009/1‑71 (MQ=255)
tGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc < 1:2996749/71‑1 (MQ=255)
cacaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACg < 1:3234888/52‑1 (MQ=255)
cattCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc < 1:3592228/66‑1 (MQ=255)
aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgca < 1:3886121/69‑2 (MQ=255)
tCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTc < 1:2760197/71‑1 (MQ=255)
tCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga > 1:3632359/1‑53 (MQ=255)
tCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAAccga > 1:457613/1‑53 (MQ=255)
tGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:3303453/1‑68 (MQ=255)
gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGt > 1:1131247/1‑40 (MQ=255)
aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcgc > 1:3706805/1‑63 (MQ=255)
aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:2801771/1‑71 (MQ=255)
aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:1252820/1‑71 (MQ=255)
aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:2860902/1‑66 (MQ=255)
aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:1301137/69‑1 (MQ=255)
aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2380664/1‑71 (MQ=255)
aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2018472/1‑71 (MQ=255)
aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:2167744/1‑71 (MQ=255)
ttttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:1880420/71‑1 (MQ=255)
tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:1066757/1‑60 (MQ=255)
tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:783581/1‑60 (MQ=255)
tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTc < 1:1525199/64‑1 (MQ=255)
ccTGATGAGAAACCGCGCACGCTACTTCACAGGTACg > 1:1191393/1‑37 (MQ=255)
cTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:184737/63‑1 (MQ=255)
cTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:585801/71‑1 (MQ=255)
tgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaa > 1:3912037/1‑71 (MQ=255)
gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:829433/1‑70 (MQ=255)
atgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac < 1:3390769/70‑2 (MQ=255)
atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAAc > 1:3402681/1‑38 (MQ=255)
atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAAc > 1:1973530/1‑38 (MQ=255)
tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACAttt < 1:3449270/47‑1 (MQ=255)
tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:3370607/1‑71 (MQ=255)
gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:1160774/1‑60 (MQ=255)
agaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:1502972/1‑71 (MQ=255)
agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt < 1:3194102/71‑1 (MQ=255)
gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:529211/1‑62 (MQ=255)
gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:2146661/1‑62 (MQ=255)
gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:3120871/1‑62 (MQ=255)
gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:2359820/1‑70 (MQ=255)
aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:2441630/1‑70 (MQ=255)
aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACa < 1:3453150/40‑1 (MQ=255)
aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc < 1:2575076/47‑1 (MQ=255)
aaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:3015079/62‑1 (MQ=255)
actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat < 1:1392119/68‑1 (MQ=255)
aCCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt > 1:777117/1‑40 (MQ=255)
ccGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:221195/1‑70 (MQ=255)
tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa > 1:729198/2‑52 (MQ=255)
tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa > 1:3328465/2‑52 (MQ=255)
tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatga > 1:3730334/2‑69 (MQ=255)
gcgcAAGCCACTTCACAGGTACGGCAACCGATACa > 1:571261/1‑35 (MQ=25)
cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:135977/1‑67 (MQ=255)
cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:201223/71‑1 (MQ=255)
gcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt < 1:384788/71‑1 (MQ=255)
gcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt > 1:2888500/1‑71 (MQ=255)
cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:2533518/71‑1 (MQ=255)
aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:850961/1‑64 (MQ=255)
aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc > 1:3439196/1‑71 (MQ=255)
aCGCTACTTCACAAGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:768890/71‑1 (MQ=255)
|
GATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTC > minE/1826192‑1826330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
