Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,636 T→A 14.5% V269V (GTA→GTT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,6360TA14.5% 285.4 / 37.1 110V269V (GTA→GTT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (49/45);  new base A (9/7);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 115 total aligned reads displayed.

GCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACC  >  minE/1933566‑1933702
                                                                      |                                                                  
gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt                                                                    <  1:3595634/71‑1 (MQ=255)
gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt                                                                    <  1:1877632/71‑1 (MQ=255)
gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt                                                                    <  1:3933959/71‑1 (MQ=255)
    gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACgg                                                                <  1:3446948/71‑1 (MQ=255)
    gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg                                                                 >  1:1757474/1‑70 (MQ=255)
    gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg                                                                 <  1:1206485/70‑1 (MQ=255)
    gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg                                                                 >  1:3189364/1‑70 (MQ=255)
     aTCAGATCTTTGTAATCTGCGTAAGCCATGAAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa                                                               <  1:1078116/71‑1 (MQ=255)
     aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa                                                               <  1:3543291/71‑1 (MQ=255)
      tCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaa                                                              <  1:666948/71‑1 (MQ=255)
      tCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa                                                               >  1:3618664/1‑70 (MQ=255)
       cAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTGCATCATGGTGAACTCTGGGTTATGACGTACGGaa                                                              <  1:1139601/70‑1 (MQ=255)
        aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa                                                             >  1:1231389/1‑70 (MQ=255)
          aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa                                                               <  1:2270945/66‑1 (MQ=255)
            cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa                                                             >  1:3423575/1‑66 (MQ=255)
              ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc                                                      >  1:3713919/1‑71 (MQ=255)
              ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc                                                      >  1:3648812/1‑71 (MQ=255)
               tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCg                                                     >  1:3843048/1‑71 (MQ=255)
               tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCg                                                     >  1:2566959/1‑71 (MQ=255)
                 tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGtt                                                   <  1:3654142/71‑1 (MQ=255)
                   aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc                                                 >  1:1800792/1‑71 (MQ=255)
                      tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt                                                            >  1:2204062/1‑57 (MQ=255)
                      tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg                                               >  1:1432986/1‑70 (MQ=255)
                        cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg                                               <  1:3747255/68‑1 (MQ=255)
                        cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg                                               <  1:3763661/68‑1 (MQ=255)
                        cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa                                             <  1:2727350/70‑1 (MQ=255)
                        cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg                                            <  1:1151388/71‑1 (MQ=255)
                          tAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg                                               >  1:1722944/1‑66 (MQ=255)
                            aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa                                         <  1:3129073/70‑1 (MQ=255)
                            aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa                                         <  1:2531258/70‑1 (MQ=255)
                             gCCGTGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg                                       >  1:898899/1‑71 (MQ=255)
                             gCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa                                         <  1:3566231/69‑1 (MQ=255)
                             gCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg                                       >  1:2009827/1‑71 (MQ=255)
                              ccATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg                                      <  1:3496383/71‑1 (MQ=255)
                              ccATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg                                      <  1:31784/71‑1 (MQ=255)
                               cATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGt                                     >  1:2432281/1‑71 (MQ=255)
                               cATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGt                                     <  1:606162/71‑1 (MQ=255)
                                aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa                                                               <  1:2149913/44‑1 (MQ=255)
                                aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt                                    <  1:3346568/71‑1 (MQ=255)
                                aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt                                    <  1:3031687/71‑1 (MQ=255)
                                 tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa                                                               <  1:1944385/43‑1 (MQ=255)
                                 tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGt                                     >  1:883771/1‑69 (MQ=255)
                                  gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTAc                                        <  1:272517/65‑1 (MQ=255)
                                   tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa                                                               <  1:3144676/41‑1 (MQ=255)
                                   tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt                                 <  1:351408/71‑1 (MQ=255)
                                    agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACTGAAGTTACGGTTGAtt                                >  1:2010645/1‑71 (MQ=255)
                                    agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt                                >  1:1596805/1‑71 (MQ=255)
                                    agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt                                >  1:3744116/1‑71 (MQ=255)
                                     gagTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAttt                               >  1:2922537/1‑71 (MQ=255)
                                     gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt                                <  1:3868686/70‑1 (MQ=255)
                                     gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt                                <  1:3854885/70‑1 (MQ=255)
                                      agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg                                      <  1:2750015/63‑1 (MQ=255)
                                      agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc                              <  1:2960355/71‑1 (MQ=255)
                                      agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc                              >  1:1255903/1‑71 (MQ=255)
                                      agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc                              <  1:2596932/71‑1 (MQ=255)
                                       gTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTc                              >  1:1558291/1‑70 (MQ=255)
                                       gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt                                 >  1:1976200/1‑67 (MQ=255)
                                       gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg                             <  1:922304/71‑1 (MQ=255)
                                       gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg                             <  1:3881830/71‑1 (MQ=255)
                                       gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg                             <  1:2235807/71‑1 (MQ=255)
                                        ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt                                    >  1:3804349/1‑63 (MQ=255)
                                        ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg                             >  1:1794347/1‑70 (MQ=255)
                                        ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg                             >  1:3181857/1‑70 (MQ=255)
                                         tCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa                           >  1:466830/1‑71 (MQ=255)
                                          ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc                                                 >  1:92746/1‑48 (MQ=255)
                                          ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa                           <  1:3432322/70‑1 (MQ=255)
                                            atcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTAccg                                      >  1:1370776/1‑55 (MQ=255)
                                            atcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc                                                      >  1:1768892/1‑41 (MQ=255)
                                            atcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc                                                      >  1:2138235/1‑41 (MQ=255)
                                            atcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc                                                      >  1:767046/1‑41 (MQ=255)
                                              catTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATaccc                      >  1:1453502/1‑69 (MQ=255)
                                              catGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa                           >  1:442877/1‑66 (MQ=255)
                                               atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa                                                  >  1:2546357/1‑42 (MQ=255)
                                                ttgTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCgt                    <  1:2639753/69‑2 (MQ=255)
                                                tGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt                                    >  1:1989752/1‑55 (MQ=255)
                                                  gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc                  >  1:1442235/1‑71 (MQ=255)
                                                   tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa                           >  1:3324809/1‑61 (MQ=255)
                                                   tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg                 <  1:249991/71‑1 (MQ=255)
                                                   tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg                 <  1:3220104/71‑1 (MQ=255)
                                                   tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg                 <  1:2740201/71‑1 (MQ=255)
                                                     aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg                 >  1:3250418/1‑69 (MQ=255)
                                                     aaCTCAGGATTATGGCGAAGAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa                           >  1:1545477/1‑59 (MQ=255)
                                                      aCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa              <  1:3645128/71‑2 (MQ=255)
                                                       ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc             >  1:3670150/1‑71 (MQ=255)
                                                       ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc             <  1:843694/71‑1 (MQ=255)
                                                       ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc             >  1:3504748/1‑71 (MQ=255)
                                                       ctcaGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGa                            >  1:1400569/1‑56 (MQ=255)
                                                         ctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca           >  1:1724942/1‑71 (MQ=255)
                                                             attATGGCGAACAGAAATACCTTCATTACGGAAGtt                                          <  1:1261407/35‑1 (MQ=16)
                                                              ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctac      <  1:922462/71‑1 (MQ=255)
                                                              ttATGACGTACGGAAATACCTTCGTTAGGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaac      <  1:1063126/71‑1 (MQ=255)
                                                               tATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATac                        >  1:2349790/1‑52 (MQ=255)
                                                                 tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac   <  1:1300805/71‑1 (MQ=255)
                                                                  gACGTACGGATATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa               >  1:1279412/1‑58 (MQ=255)
                                                                  gACGTACGGATATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa               >  1:1274816/1‑58 (MQ=255)
                                                                   aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccacc  <  1:3086059/70‑1 (MQ=255)
                                                                    cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac   >  1:1376230/1‑68 (MQ=255)
                                                                     gTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca           >  1:189450/1‑59 (MQ=255)
                                                                      tACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt                                >  1:3913098/1‑37 (MQ=255)
                                                                      tACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt                                >  1:1625328/1‑37 (MQ=255)
                                                                      |                                                                  
GCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACC  >  minE/1933566‑1933702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A