Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,933,684 |
C→T |
20.7% |
E253E (GAG→GAA) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,933,684 | 0 | C | T | 20.7%
| 182.8
/ 40.1
| 87 | E253E (GAG→GAA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (38/31); new base T (10/8); total (48/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
GTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCC > minE/1933616‑1933753
|
gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:1442235/1‑71 (MQ=255)
tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:3220104/71‑1 (MQ=255)
tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:2740201/71‑1 (MQ=255)
tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:249991/71‑1 (MQ=255)
aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:3250418/1‑69 (MQ=255)
aCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa < 1:3645128/71‑2 (MQ=255)
ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:3504748/1‑71 (MQ=255)
ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:3670150/1‑71 (MQ=255)
ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:843694/71‑1 (MQ=255)
ctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:1724942/1‑71 (MQ=255)
tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:3473895/1‑71 (MQ=255)
ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctac < 1:922462/71‑1 (MQ=255)
ttATGACGTACGGAAATACCTTCGTTAGGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaac < 1:1063126/71‑1 (MQ=255)
tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:1300805/71‑1 (MQ=255)
gACGTACGGATATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa > 1:1279412/1‑58 (MQ=255)
gACGTACGGATATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa > 1:1274816/1‑58 (MQ=255)
aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccacc < 1:3086059/70‑1 (MQ=255)
cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:1376230/1‑68 (MQ=255)
cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:3630647/1‑68 (MQ=255)
gTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:189450/1‑59 (MQ=255)
aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:3649797/1‑71 (MQ=255)
cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAg > 1:629623/3‑67 (MQ=255)
cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt > 1:3141714/3‑70 (MQ=255)
cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:2166419/1‑71 (MQ=255)
ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:2088152/1‑71 (MQ=255)
ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:1701368/1‑71 (MQ=255)
agAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgtt < 1:1161142/70‑3 (MQ=255)
agAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgtt < 1:3260411/70‑3 (MQ=255)
gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt > 1:2154592/1‑71 (MQ=255)
aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGAc < 1:3478955/66‑1 (MQ=255)
cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:2315807/71‑1 (MQ=255)
ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaac > 1:2638097/1‑42 (MQ=25)
ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:3514976/1‑69 (MQ=255)
tcattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAg > 1:3712641/4‑71 (MQ=255)
cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGt > 1:2433687/1‑71 (MQ=255)
attACGGAAGTTACGGTTGATTTCGAATACCCGTTc < 1:962862/35‑1 (MQ=37)
attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTAcaacca < 1:3163864/52‑1 (MQ=255)
ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCt < 1:3123190/58‑1 (MQ=255)
ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc < 1:1908235/71‑1 (MQ=255)
tACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGa > 1:1414325/1‑53 (MQ=255)
aCGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa > 1:984208/1‑36 (MQ=25)
aCGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa > 1:2408405/1‑36 (MQ=25)
aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc < 1:495593/70‑1 (MQ=255)
ggAAGTTACGGTTGATTTCGAATACACTCTCGAAGc < 1:2014571/36‑1 (MQ=255)
ggAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:855054/1‑36 (MQ=255)
ggAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:3189086/36‑1 (MQ=255)
ggAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgc > 1:623995/1‑71 (MQ=255)
gAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcg < 1:792838/71‑1 (MQ=255)
aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:2790031/44‑1 (MQ=255)
aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCTGACGCTTGAGGTACAGTTCCGgcgc < 1:2946860/71‑1 (MQ=255)
aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:2645146/1‑71 (MQ=255)
aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:3912495/1‑71 (MQ=255)
aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:1843283/1‑70 (MQ=255)
gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:1967341/42‑1 (MQ=255)
gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACAAGACGCTTGAGGTACAGTTCCGGCGCGa > 1:1364136/1‑71 (MQ=255)
aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc > 1:3718751/1‑71 (MQ=255)
cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc < 1:1133437/70‑1 (MQ=255)
cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:616068/71‑1 (MQ=255)
ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg > 1:1168678/1‑70 (MQ=255)
gTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCa < 1:1078146/71‑1 (MQ=255)
ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3474315/69‑1 (MQ=255)
ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg > 1:3582482/1‑71 (MQ=255)
ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg > 1:3080684/1‑71 (MQ=255)
gATTTCGAATACACGCTCGAAGCCACCAACCACCAGa > 1:2640767/1‑37 (MQ=255)
gATTTCGAATACACGCTAGAAGCCACCAACCACCAGa > 1:1152665/1‑37 (MQ=255)
aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa > 1:3416192/1‑71 (MQ=255)
aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:2983683/71‑1 (MQ=255)
tttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATa > 1:911576/1‑70 (MQ=255)
ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc > 1:1181936/1‑52 (MQ=255)
ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:3801662/1‑71 (MQ=255)
ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:1286058/1‑71 (MQ=255)
ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:1290634/1‑71 (MQ=255)
cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt < 1:3335801/70‑1 (MQ=255)
cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt < 1:638973/70‑1 (MQ=255)
cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATg > 1:1181427/1‑71 (MQ=255)
gAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGt > 1:587896/1‑71 (MQ=255)
gAATACACGCTCGAAGCCACCAACCACCAGACGCTT‑AGGTACAGTTCCGgcgc < 1:339026/53‑1 (MQ=255)
aaTACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGt < 1:1217053/70‑1 (MQ=255)
tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATcta < 1:2333770/71‑3 (MQ=255)
tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATct > 1:1983765/1‑69 (MQ=255)
tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg < 1:3768193/42‑1 (MQ=255)
tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:1214196/1‑69 (MQ=255)
tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga < 1:86483/71‑1 (MQ=255)
acacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:2422356/62‑1 (MQ=255)
cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga < 1:3669173/71‑1 (MQ=255)
gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc < 1:1093881/71‑1 (MQ=255)
gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc > 1:893998/1‑70 (MQ=255)
|
GTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCC > minE/1933616‑1933753
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
