Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,933,780 |
A→G |
12.9% |
R221R (CGT→CGC) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,933,780 | 0 | A | G | 12.9%
| 155.6
/ 14.0
| 62 | R221R (CGT→CGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (31/23); new base G (3/5); total (34/28) |
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01 |
GGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCA > minE/1933713‑1933850
|
ggTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcg > 1:782838/1‑70 (MQ=255)
gatACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcga < 1:3758614/69‑2 (MQ=255)
aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg > 1:1354442/1‑69 (MQ=255)
cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg > 1:1155071/1‑71 (MQ=255)
cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg > 1:2361368/1‑71 (MQ=255)
aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGc < 1:1819896/71‑1 (MQ=255)
gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg < 1:3654387/71‑1 (MQ=255)
gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg > 1:1475851/1‑71 (MQ=255)
tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg > 1:3460421/1‑70 (MQ=255)
gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc < 1:2264892/71‑1 (MQ=255)
gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc < 1:2126745/71‑1 (MQ=255)
gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc > 1:3251873/1‑70 (MQ=255)
gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg < 1:3706680/71‑1 (MQ=255)
gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg < 1:1788250/71‑1 (MQ=255)
tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg > 1:1957852/1‑70 (MQ=255)
aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa > 1:453053/1‑71 (MQ=255)
cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa < 1:880894/70‑1 (MQ=255)
gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa < 1:3910733/69‑1 (MQ=255)
aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc > 1:516773/1‑71 (MQ=255)
gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg < 1:2381602/63‑1 (MQ=255)
gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt > 1:2798574/1‑71 (MQ=255)
gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt > 1:1026421/1‑71 (MQ=255)
aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAc > 1:1075801/1‑40 (MQ=255)
aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc < 1:3224735/63‑1 (MQ=255)
gTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa > 1:1257388/1‑49 (MQ=255)
cGAGCTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa > 1:431986/1‑47 (MQ=255)
cGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAcc > 1:1575550/1‑49 (MQ=255)
cGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCatcatc > 1:2247221/1‑71 (MQ=255)
gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg < 1:1550066/71‑1 (MQ=255)
tCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCg < 1:214493/70‑1 (MQ=255)
cAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGttt < 1:3709489/71‑1 (MQ=255)
aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGttt > 1:1721954/1‑70 (MQ=255)
aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc < 1:3296067/71‑1 (MQ=255)
gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCg < 1:3218267/35‑1 (MQ=255)
cgcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa > 1:2246622/1‑71 (MQ=255)
attATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACtt > 1:3654041/2‑71 (MQ=255)
tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc > 1:3135061/1‑70 (MQ=255)
tggtggGCGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt < 1:3850904/71‑1 (MQ=255)
tgatgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTcc > 1:2541611/4‑69 (MQ=255)
gtgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATa > 1:2177956/1‑70 (MQ=255)
atgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATa < 1:2810000/69‑1 (MQ=255)
atgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATa < 1:3696467/69‑1 (MQ=255)
tgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATa > 1:1411182/1‑69 (MQ=255)
gggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAg > 1:1414807/1‑71 (MQ=255)
ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAg < 1:2314223/70‑1 (MQ=255)
ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc > 1:3260724/1‑71 (MQ=255)
gtgATAAACGGACGCGCAGCGGCTCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt > 1:1454886/1‑60 (MQ=255)
tgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatcat < 1:1989731/46‑1 (MQ=255)
aTAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgc < 1:3420029/70‑1 (MQ=255)
tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg > 1:2073661/1‑71 (MQ=255)
tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg > 1:3834172/1‑71 (MQ=255)
aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcg > 1:269631/1‑69 (MQ=255)
aaaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGt > 1:333916/1‑71 (MQ=255)
aaaCCGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCgg > 1:2977140/1‑45 (MQ=255)
aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat > 1:1976026/1‑38 (MQ=255)
aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat > 1:232042/1‑38 (MQ=255)
aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGtt < 1:3052754/71‑1 (MQ=255)
gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1816317/71‑1 (MQ=255)
gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1877772/71‑1 (MQ=255)
gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:3683957/71‑1 (MQ=255)
gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1664655/71‑1 (MQ=255)
gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1859456/71‑1 (MQ=255)
aCGCGCAGCGGCACCGCCAGGGATCACGTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCa > 1:1597963/1‑71 (MQ=255)
|
GGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCA > minE/1933713‑1933850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
