Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,969 G→A 22.8% H158H (CAC→CAT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,9690GA22.8% 134.0 / 36.0 70H158H (CAC→CAT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (24/30);  new base A (11/5);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

GCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTT  >  minE/1933900‑1934039
                                                                     |                                                                      
gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt                                                                       >  1:3877097/1‑71 (MQ=255)
gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATACTGCAAGCCg                                                                        <  1:1179262/70‑1 (MQ=255)
 cGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt                                                                       <  1:3171502/70‑1 (MQ=255)
   ggATTCTTCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt                                                                       <  1:805195/68‑1 (MQ=255)
   ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt                                                                       <  1:2804304/68‑1 (MQ=255)
   ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg                                                                     >  1:3499569/1‑70 (MQ=255)
      ttCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt                                                                 >  1:3209376/1‑71 (MQ=255)
       ttaTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAtt                                                                 >  1:606104/3‑70 (MQ=255)
         aTCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt                                                              >  1:3705092/1‑71 (MQ=255)
            gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc                                                            <  1:2830040/70‑1 (MQ=255)
            gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc                                                            <  1:1311780/70‑1 (MQ=255)
             ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg                                                          <  1:3227969/71‑1 (MQ=255)
                 cgatgaggtCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTggta                                                        >  1:2147575/2‑67 (MQ=255)
                  gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg                                                     >  1:1843248/1‑71 (MQ=255)
                   atgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa                                                                    <  1:1865289/55‑1 (MQ=255)
                   atgagatCGAGATAACGCTGACAATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt                                                              >  1:1278203/1‑61 (MQ=255)
                      agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATc                                                             >  1:210819/1‑59 (MQ=255)
                      agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg                                                     >  1:1281842/1‑67 (MQ=255)
                      agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg                                                 >  1:2977955/1‑71 (MQ=255)
                        atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt                                                                 <  1:1400419/53‑1 (MQ=255)
                        atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg                                                       <  1:486779/63‑1 (MQ=255)
                            agaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGt                                             >  1:1346340/1‑69 (MQ=255)
                            agaTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt                                                                <  1:366453/50‑1 (MQ=255)
                             gaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgt                                                >  1:2572066/1‑64 (MQ=255)
                              aTAACGCTGACGATAGCGCGCTTCCTGAGCCTGCAAGCCGTGGAATTTATCCGGc                                                         <  1:2164308/55‑1 (MQ=255)
                               tAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt                                         >  1:1584586/1‑70 (MQ=255)
                                aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTa                                                               >  1:2165279/1‑47 (MQ=255)
                                aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTa                                                               >  1:2402927/1‑47 (MQ=255)
                                 aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                       <  1:3901868/70‑2 (MQ=255)
                                 aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                       <  1:3022798/70‑2 (MQ=255)
                                 aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCCGCGGACGCAGTGCTTTgg                                      <  1:1927494/71‑1 (MQ=255)
                                 aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg                                       <  1:3189770/70‑1 (MQ=255)
                                  cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg                                                                     <  1:2426346/39‑1 (MQ=255)
                                  cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                     >  1:3601980/1‑71 (MQ=255)
                                   gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                     <  1:3547776/70‑1 (MQ=255)
                                     tGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                  >  1:119124/1‑71 (MQ=255)
                                      gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgta                                               >  1:3114064/1‑55 (MQ=255)
                                        cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagcag                               <  1:2671373/71‑1 (MQ=255)
                                        cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                  >  1:2228930/1‑68 (MQ=255)
                                         gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt                                                              >  1:3690804/1‑39 (MQ=255)
                                         gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt                                                              >  1:1529379/1‑39 (MQ=255)
                                         gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt                                                              >  1:1524875/1‑39 (MQ=255)
                                         gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa                              <  1:1193225/71‑1 (MQ=255)
                                           tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc                                    >  1:2968468/1‑63 (MQ=255)
                                             gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc                           <  1:1660476/70‑1 (MQ=255)
                                              cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                     <  1:1574511/59‑1 (MQ=255)
                                              cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                         <  1:3551784/71‑1 (MQ=255)
                                              cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                         <  1:3120687/71‑1 (MQ=255)
                                                 acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTca                                   <  1:1779040/57‑1 (MQ=255)
                                                 acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCag                         >  1:2363474/2‑67 (MQ=255)
                                                  cTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                     >  1:3761438/1‑71 (MQ=255)
                                                    tCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTcag                    >  1:3840113/1‑68 (MQ=255)
                                                    tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                   >  1:909015/1‑71 (MQ=255)
                                                     ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                  >  1:3627898/1‑71 (MQ=255)
                                                     ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAAGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                  >  1:538962/1‑71 (MQ=255)
                                                      cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCgg                    >  1:1742852/1‑68 (MQ=255)
                                                      cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                   >  1:2585507/1‑69 (MQ=255)
                                                      cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                 <  1:1109558/71‑1 (MQ=255)
                                                        gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                       <  1:305944/63‑1 (MQ=255)
                                                        gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                       <  1:1777733/63‑1 (MQ=255)
                                                         aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                <  1:347170/69‑1 (MQ=255)
                                                         aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                <  1:384925/69‑1 (MQ=255)
                                                         aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCa                <  1:1158369/69‑1 (MQ=255)
                                                         aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt              >  1:2764946/1‑71 (MQ=255)
                                                         aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt              <  1:1279800/71‑1 (MQ=255)
                                                         aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt              <  1:697177/71‑1 (MQ=255)
                                                          tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                  >  1:1865291/1‑50 (MQ=255)
                                                            cTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa           <  1:499918/71‑1 (MQ=255)
                                                                 aGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc      <  1:105514/71‑1 (MQ=255)
                                                                  gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa       >  1:2930630/1‑69 (MQ=255)
                                                                    cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg             <  1:1832278/61‑1 (MQ=255)
                                                                     gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt  >  1:2548821/1‑71 (MQ=255)
                                                                     gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt  >  1:1145555/1‑71 (MQ=255)
                                                                     |                                                                      
GCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTT  >  minE/1933900‑1934039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A