Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,986 G→A 19.1% L153L (CTG→TTG) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,9860GA19.1% 147.1 / 27.9 68L153L (CTG→TTG) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (24/31);  new base A (8/5);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTG  >  minE/1933918‑1934056
                                                                    |                                                                      
gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg                                                                      >  1:1843248/1‑71 (MQ=255)
    agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg                                                                      >  1:1281842/1‑67 (MQ=255)
    agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg                                                                  >  1:2977955/1‑71 (MQ=255)
      atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg                                                                        <  1:486779/63‑1 (MQ=255)
          agaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGt                                                              >  1:1346340/1‑69 (MQ=255)
           gaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgt                                                                 >  1:2572066/1‑64 (MQ=255)
             tAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt                                                          >  1:1584586/1‑70 (MQ=255)
               aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                                        <  1:3022798/70‑2 (MQ=255)
               aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                                        <  1:3901868/70‑2 (MQ=255)
               aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCCGCGGACGCAGTGCTTTgg                                                       <  1:1927494/71‑1 (MQ=255)
               aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg                                                        <  1:3189770/70‑1 (MQ=255)
                cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                      >  1:3601980/1‑71 (MQ=255)
                 gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                      <  1:3547776/70‑1 (MQ=255)
                   tGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                   >  1:119124/1‑71 (MQ=255)
                    gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgta                                                                >  1:3114064/1‑55 (MQ=255)
                      cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagcag                                                <  1:2671373/71‑1 (MQ=255)
                      cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                                   >  1:2228930/1‑68 (MQ=255)
                       gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa                                               <  1:1193225/71‑1 (MQ=255)
                         tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc                                                     >  1:2968468/1‑63 (MQ=255)
                           gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc                                            <  1:1660476/70‑1 (MQ=255)
                            cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                      <  1:1574511/59‑1 (MQ=255)
                            cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                                          <  1:3120687/71‑1 (MQ=255)
                            cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                                          <  1:3551784/71‑1 (MQ=255)
                               acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTca                                                    <  1:1779040/57‑1 (MQ=255)
                               acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCag                                          >  1:2363474/2‑67 (MQ=255)
                                cTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                      >  1:3761438/1‑71 (MQ=255)
                                  tCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTcag                                     >  1:3840113/1‑68 (MQ=255)
                                  tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                                    >  1:909015/1‑71 (MQ=255)
                                   ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                                   >  1:3627898/1‑71 (MQ=255)
                                   ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAAGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                                   >  1:538962/1‑71 (MQ=255)
                                    cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCgg                                     >  1:1742852/1‑68 (MQ=255)
                                    cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                                    >  1:2585507/1‑69 (MQ=255)
                                    cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                  <  1:1109558/71‑1 (MQ=255)
                                      gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                        <  1:305944/63‑1 (MQ=255)
                                      gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                        <  1:1777733/63‑1 (MQ=255)
                                       aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                                 <  1:347170/69‑1 (MQ=255)
                                       aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                                 <  1:384925/69‑1 (MQ=255)
                                       aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCa                                 <  1:1158369/69‑1 (MQ=255)
                                       aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                               <  1:1279800/71‑1 (MQ=255)
                                       aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                               >  1:2764946/1‑71 (MQ=255)
                                       aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                               <  1:697177/71‑1 (MQ=255)
                                        tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                                   >  1:1865291/1‑50 (MQ=255)
                                          cTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa                            <  1:499918/71‑1 (MQ=255)
                                               aGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                       <  1:105514/71‑1 (MQ=255)
                                                gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                        >  1:2930630/1‑69 (MQ=255)
                                                  cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                              <  1:1832278/61‑1 (MQ=255)
                                                   gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt                   >  1:2548821/1‑71 (MQ=255)
                                                   gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt                   >  1:1145555/1‑71 (MQ=255)
                                                    tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgct                   >  1:2314831/1‑68 (MQ=255)
                                                    tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACg                                             >  1:3311014/1‑44 (MQ=255)
                                                    tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                      >  1:697659/1‑51 (MQ=255)
                                                    tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa                            >  1:1263051/1‑61 (MQ=255)
                                                    tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa                      >  1:1297210/1‑67 (MQ=255)
                                                     ggAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                  <  1:788719/54‑1 (MQ=255)
                                                      gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGc                <  1:2404134/71‑1 (MQ=255)
                                                          ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCgg             <  1:1235123/70‑1 (MQ=255)
                                                             tCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt         <  1:1756259/71‑1 (MQ=255)
                                                             tCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttt          <  1:295452/70‑1 (MQ=255)
                                                              ccGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttg        >  1:3790899/1‑71 (MQ=255)
                                                              ccGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt         >  1:1277966/1‑70 (MQ=255)
                                                               cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg       >  1:1025603/1‑71 (MQ=255)
                                                               cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg       <  1:3674647/71‑1 (MQ=255)
                                                               cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg       <  1:3670154/71‑1 (MQ=255)
                                                                ggCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggt      <  1:676283/71‑1 (MQ=255)
                                                                 gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                  <  1:1318606/42‑1 (MQ=255)
                                                                 gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt     <  1:1514901/71‑1 (MQ=255)
                                                                 gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt     <  1:1181166/71‑1 (MQ=255)
                                                                 gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt     <  1:1042513/71‑1 (MQ=255)
                                                                 gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt         <  1:1856460/67‑1 (MQ=255)
                                                                    gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg  <  1:3493045/71‑1 (MQ=255)
                                                                    gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg  <  1:2485912/71‑1 (MQ=255)
                                                                    gCGGACGCAGTGCTTTGGTCAGCAGACGCAACGCGGTGCAg                                <  1:1690924/41‑1 (MQ=255)
                                                                    gCGGACGCAGGGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg  <  1:444897/71‑1 (MQ=255)
                                                                    |                                                                      
GATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTG  >  minE/1933918‑1934056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A