Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,220,199 |
C→T |
15.8% |
intergenic (+459/‑519) |
yhdH → / → accB |
predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,220,199 | 0 | C | T | 15.8%
| 150.4
/ 17.9
| 63 | intergenic (+459/‑519) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base T (5/5); total (34/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01 |
CAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTG > minE/2220131‑2220268
|
cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt < 1:1963329/71‑1 (MQ=255)
aGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg > 1:1632614/1‑71 (MQ=255)
aCGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc < 1:1735119/71‑1 (MQ=255)
cGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCa > 1:3223493/1‑71 (MQ=255)
gATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc > 1:3273377/1‑69 (MQ=255)
tGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCATGTCGCGATCCTGGCATcc > 1:725309/1‑71 (MQ=255)
tGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGc > 1:2068044/1‑67 (MQ=255)
cAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc > 1:2237226/1‑70 (MQ=255)
aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat > 1:1959895/1‑70 (MQ=255)
aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:537918/71‑1 (MQ=255)
aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:3355265/1‑68 (MQ=255)
aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:362339/1‑71 (MQ=255)
aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:1730527/1‑71 (MQ=255)
gggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtg < 1:3593622/70‑1 (MQ=255)
ggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:3064823/68‑1 (MQ=255)
tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTgg > 1:899234/1‑70 (MQ=255)
aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:3277027/1‑71 (MQ=255)
aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:1333357/1‑71 (MQ=255)
tCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCt > 1:1493443/1‑70 (MQ=255)
cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa > 1:3750518/1‑53 (MQ=255)
cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:2076917/71‑1 (MQ=255)
gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:3631409/38‑1 (MQ=255)
gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgt > 1:746696/1‑66 (MQ=255)
gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAg < 1:2025432/70‑1 (MQ=255)
aaacGCTGCGTAATGGCGTCTATCTTGTCGCGATCCTGGCTTGCCTACATTATTTGTGGTCTTTGAAGAtt < 1:932014/68‑1 (MQ=255)
aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATc > 1:1554008/1‑35 (MQ=255)
aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:3621841/71‑1 (MQ=255)
aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:1592181/71‑1 (MQ=255)
aCCGCTGCGTAATGTCGTCTATCTTGTCGCGATcg < 1:114431/35‑2 (MQ=255)
cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc < 1:560546/71‑1 (MQ=255)
gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:181925/1‑45 (MQ=255)
gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACa > 1:3457964/1‑45 (MQ=255)
gcatAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGAAGATTATTTCa < 1:2331853/67‑1 (MQ=255)
gCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt > 1:3208725/1‑71 (MQ=255)
cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:1021865/69‑1 (MQ=255)
cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:3613836/69‑1 (MQ=255)
cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAtt < 1:1665413/71‑1 (MQ=255)
tAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:2851323/35‑1 (MQ=255)
aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg < 1:3578032/71‑1 (MQ=255)
aTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAg < 1:3357673/71‑1 (MQ=255)
tGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattat < 1:2685490/40‑1 (MQ=255)
cgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccc > 1:43452/1‑71 (MQ=255)
gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcccat > 1:2706800/1‑69 (MQ=255)
gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCt > 1:1692820/1‑71 (MQ=255)
cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCa > 1:263531/1‑71 (MQ=255)
tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCAt > 1:1218499/1‑71 (MQ=255)
tATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGAAGCCGCTCa > 1:1786018/1‑70 (MQ=255)
tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAt > 1:2893/1‑71 (MQ=255)
aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg < 1:113801/38‑1 (MQ=255)
aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg < 1:3500207/38‑1 (MQ=255)
aTCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg < 1:1514676/38‑1 (MQ=255)
aTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATc > 1:3737366/1‑71 (MQ=255)
tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt > 1:1963595/1‑71 (MQ=255)
cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGt > 1:719520/1‑37 (MQ=255)
gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc < 1:2189772/69‑1 (MQ=255)
tCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt < 1:1790647/37‑1 (MQ=255)
tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg < 1:3474209/53‑1 (MQ=255)
tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa < 1:2276963/71‑1 (MQ=255)
cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:1734788/1‑36 (MQ=255)
cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:2477830/1‑36 (MQ=255)
cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGa > 1:2482351/1‑36 (MQ=255)
cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:2946723/70‑1 (MQ=255)
aTCCTGGCATCCCTACATTATTTGTGGTTTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:2966677/1‑71 (MQ=255)
aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:3882101/1‑71 (MQ=255)
tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTa < 1:3620748/39‑1 (MQ=255)
tCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctg < 1:2441963/71‑1 (MQ=255)
|
CAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTG > minE/2220131‑2220268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
