Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
797,006 |
G→A |
21.7% |
G11S (GGC→AGC) |
modC → |
molybdate transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 797,006 | 0 | G | A | 21.7%
| 94.6
/ 22.1
| 46 | G11S (GGC→AGC) | modC | molybdate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (12/24); new base A (3/7); total (15/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01 |
GCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCGTCTCC > W3110S.gb/796937‑797074
|
gctggcCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTgg < 1:207396/70‑1 (MQ=255)
gcCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTgg > 1:4124604/1‑66 (MQ=255)
gcCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:2351784/71‑1 (MQ=255)
gcCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCa < 1:4718858/69‑1 (MQ=255)
cAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:385702/69‑1 (MQ=255)
gAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCAtt < 1:788284/71‑1 (MQ=255)
tCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCa < 1:4893782/66‑1 (MQ=255)
gTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCAtt > 1:3170889/1‑62 (MQ=255)
gTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCAtt > 1:475544/1‑62 (MQ=255)
aaCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:718584/55‑1 (MQ=255)
aaCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTg > 1:2747920/1‑64 (MQ=255)
aaCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTAtt < 1:4129574/70‑1 (MQ=255)
aaCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTAtt < 1:4659871/70‑1 (MQ=255)
aaCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCTGACTAtt < 1:2440518/70‑1 (MQ=255)
aaCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCTGACTAtt < 1:201602/70‑1 (MQ=255)
aCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTAtt < 1:180961/69‑1 (MQ=255)
aCGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCAATGCCTGACTAtt < 1:694953/69‑1 (MQ=255)
cgggcgggGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGc > 1:2918099/1‑50 (MQ=255)
cgggcgggGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:2764083/53‑1 (MQ=255)
cggggcgCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGaaa < 1:2041869/71‑1 (MQ=255)
cgcTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCt < 1:43203/70‑1 (MQ=255)
gcTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCt < 1:1392379/51‑1 (MQ=255)
gcTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCt < 1:5080905/51‑1 (MQ=255)
cTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATg < 1:4663372/61‑1 (MQ=255)
cTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATg < 1:4501948/61‑1 (MQ=255)
cTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATg < 1:2728311/61‑1 (MQ=255)
tAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCTGa > 1:157344/1‑51 (MQ=255)
tAATCATGCTGGAACTGAATTTTTCCCAGACGTTGAGCAACCATTGCCTGa > 1:1960794/1‑51 (MQ=255)
aaTCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCg > 1:3640315/1‑71 (MQ=255)
cATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:4411283/37‑1 (MQ=255)
cATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:361854/37‑1 (MQ=255)
cATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAc < 1:2791769/37‑1 (MQ=255)
cATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGcc > 1:3408397/1‑70 (MQ=255)
aTGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGaaa > 1:1559301/1‑58 (MQ=255)
tGCTGGAACTGAATTTTTCCCAGACGTTGGGCAAcc < 1:443200/36‑1 (MQ=255)
ggAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGa > 1:687883/1‑41 (MQ=255)
gAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCAt < 1:1342409/71‑1 (MQ=255)
aCTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCa > 1:4971091/1‑71 (MQ=255)
tGAATTTTTCCCAGACGTTGAGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAAt < 1:2031860/62‑1 (MQ=255)
gAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCa > 1:492679/1‑68 (MQ=255)
gAATTTTTCCCAGACGTTGAGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATc < 1:1032835/67‑1 (MQ=255)
aTTTTTCCCAGACGTTGAGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCAc > 1:4951892/1‑67 (MQ=255)
tttCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCAc > 1:2082545/1‑64 (MQ=255)
tCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCtt > 1:4044806/1‑71 (MQ=255)
cccAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCtt < 1:1060978/70‑1 (MQ=255)
ccAGACGTTGGGCAACCATTGCCTGACTATTAATGAAAc < 1:2801837/39‑1 (MQ=255)
ccAGACGTTGGGCAACCATTGCCTGACTATTAATGAAAc < 1:4040033/39‑1 (MQ=255)
aCGTTGGGCAACCATTGCCTGACTATTAATGAAAc < 1:5081135/35‑1 (MQ=255)
gggCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCGTCTcc < 1:3393008/70‑1 (MQ=255)
|
GCTGGCCAGAATCAGCCGTGAACGGGCGGGGCGCTAATCATGCTGGAACTGAATTTTTCCCAGACGTTGGGCAACCATTGCCTGACTATTAATGAAACGCTGCCCGCCAATGGCATCACTGCTATCTTTGGCGTCTCC > W3110S.gb/796937‑797074
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
