Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,874,182 |
A→G |
20.0% |
Y143C (TAC→TGC) |
yeaJ → |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,874,182 | 0 | A | G | 20.0%
| 117.8
/ 24.8
| 55 | Y143C (TAC→TGC) | yeaJ | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (25/19); new base G (9/2); total (34/21) |
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAT > W3110S.gb/1874114‑1874252
|
tgtgGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCg > 1:805491/1‑71 (MQ=255)
gtgGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACgg > 1:424133/1‑71 (MQ=255)
aaGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGtt < 1:3099386/69‑1 (MQ=255)
aGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAg < 1:4235728/71‑1 (MQ=255)
aGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAg < 1:1585004/71‑1 (MQ=255)
gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTa > 1:2877172/1‑56 (MQ=255)
gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTgg > 1:3921494/1‑71 (MQ=255)
gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTgg < 1:3145163/71‑1 (MQ=255)
cTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGa > 1:548318/1‑70 (MQ=255)
cTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGa > 1:3045783/1‑70 (MQ=255)
cTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGa > 1:3682977/1‑70 (MQ=255)
aTTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATgg > 1:2759292/1‑71 (MQ=255)
aCCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGcc > 1:4197906/1‑70 (MQ=255)
aCCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGcc > 1:132282/1‑70 (MQ=255)
cccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCg > 1:1670796/1‑70 (MQ=255)
cccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCg > 1:1611585/1‑70 (MQ=255)
cccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCg > 1:3887574/1‑70 (MQ=255)
ccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:3340990/71‑1 (MQ=255)
ccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:1231452/71‑1 (MQ=255)
ccGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:1992890/71‑1 (MQ=255)
cGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCg > 1:1787164/1‑68 (MQ=255)
cGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa < 1:3965565/70‑1 (MQ=255)
gCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGa > 1:3616785/1‑61 (MQ=255)
gCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa > 1:4394330/1‑69 (MQ=255)
cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAagag < 1:4475463/71‑1 (MQ=255)
ttGATCAGTCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAgg > 1:156843/1‑49 (MQ=255)
ttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTa > 1:2139802/1‑40 (MQ=255)
aGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtat > 1:3316534/1‑70 (MQ=255)
ggAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTAtc > 1:1583428/1‑71 (MQ=255)
gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:3783933/1‑41 (MQ=255)
gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:3692006/1‑41 (MQ=255)
gAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGaa > 1:3028148/1‑51 (MQ=255)
aCCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAtct > 1:2804919/1‑70 (MQ=255)
aCCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAtctc < 1:450478/71‑1 (MQ=255)
ccGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCa < 1:2656255/71‑1 (MQ=255)
cGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt > 1:78905/1‑50 (MQ=255)
cGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCaa > 1:3378141/1‑71 (MQ=255)
gCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAAt < 1:3183324/71‑1 (MQ=255)
accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCaa > 1:1071814/1‑67 (MQ=255)
accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCaa > 1:1325215/1‑67 (MQ=255)
accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGAc < 1:374875/71‑1 (MQ=255)
accaGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGAc < 1:3138371/71‑1 (MQ=255)
caGGATGATTACGGTTCAGGTCTTGGGATGGCCGa < 1:701696/35‑1 (MQ=255)
caGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt > 1:2660167/1‑60 (MQ=255)
caGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt > 1:4920366/1‑60 (MQ=255)
caGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATATCACTATTATCTCGAt > 1:2505926/1‑60 (MQ=255)
aGGATGATTACGGTTCAGGTCTTTGGATGGCCGAAGAGAAATTTCACta > 1:1174594/1‑49 (MQ=255)
aGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGaaa < 1:4271932/41‑1 (MQ=255)
aGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGaaa < 1:5081759/41‑1 (MQ=255)
aGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACta > 1:4692868/1‑49 (MQ=255)
ggATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGc > 1:3958004/1‑70 (MQ=255)
gatgatTACGGTTCAGGTCTTGGGATGGCGGAAGAGAAATTTCAc < 1:761343/45‑1 (MQ=255)
gatgatTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCAc < 1:4942196/45‑1 (MQ=255)
tgatTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt > 1:1166765/1‑55 (MQ=255)
gatTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtattat > 1:4423350/1‑48 (MQ=255)
atTGCGGTTCAGGTCTTGGGATGGCCTAAGAGAAATTTCACTATTAtc < 1:5140272/48‑1 (MQ=255)
atTGCGGGTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTAtc < 1:1590775/48‑1 (MQ=255)
aCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAt > 1:2464542/1‑71 (MQ=255)
|
TGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAT > W3110S.gb/1874114‑1874252
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
