Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,874,182 |
A→G |
Y143C (TAC→TGC) |
yeaJ → |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,874,182 | 0 | A | G | 100.0%
| 155.4
/ NA
| 42 | Y143C (TAC→TGC) | yeaJ | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/30); total (12/30) |
CAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAT > W3110S.gb/1874125‑1874231
|
cagcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCgg > 1:1643156/1‑61 (MQ=255)
cagcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCgg > 1:4222728/1‑61 (MQ=255)
cagcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCgg > 1:2005842/1‑61 (MQ=255)
cagcaCTATTACCCGCTTTTGATCAGGAAGTGGAAGGCAACCGCCACCAGGATGATTGCgg > 1:3713620/1‑61 (MQ=255)
gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGt < 1:1581811/60‑1 (MQ=255)
aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:4535568/62‑1 (MQ=255)
aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:1728966/62‑1 (MQ=255)
aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:179887/62‑1 (MQ=255)
aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:3795596/62‑1 (MQ=255)
aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:227339/62‑1 (MQ=255)
ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGTATGATTGCGGTTCAg < 1:2467299/58‑1 (MQ=255)
ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:2547634/58‑1 (MQ=255)
ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:833577/58‑1 (MQ=255)
ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg < 1:1875688/58‑1 (MQ=255)
cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:862121/1‑58 (MQ=255)
cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:2468406/1‑58 (MQ=255)
cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg > 1:4553889/1‑58 (MQ=255)
ttttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg < 1:2975185/62‑1 (MQ=255)
ttttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg < 1:3461867/62‑1 (MQ=255)
ttttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg < 1:157907/62‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg < 1:3392966/61‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg < 1:4074376/61‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:1573977/62‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:1163379/62‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:3417681/62‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:1855889/62‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:3746902/62‑1 (MQ=255)
tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:4636138/62‑1 (MQ=255)
ttGATCAGGCTGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc < 1:725579/61‑1 (MQ=255)
aGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAaga > 1:4596828/1‑61 (MQ=255)
ggCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAgaga > 1:1470646/1‑62 (MQ=255)
gTGGAAGTCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt < 1:2774816/62‑1 (MQ=255)
gTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt < 1:2893773/62‑1 (MQ=255)
gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt < 1:3691817/59‑1 (MQ=255)
gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt < 1:3346035/59‑1 (MQ=255)
aCCGCCACCAGGATGATTGCGGTTCAGGTCTTggg > 1:1260801/1‑35 (MQ=255)
aCCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtat > 1:4583418/1‑59 (MQ=255)
aCCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtat > 1:1942061/1‑59 (MQ=255)
cGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTAtct < 1:2802776/62‑1 (MQ=255)
caccaGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGa < 1:2451811/62‑1 (MQ=255)
caccaGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGa < 1:3144743/62‑1 (MQ=255)
accaGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt < 1:412637/62‑1 (MQ=255)
|
CAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAT > W3110S.gb/1874125‑1874231
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
