Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
801,414 |
A→G |
53.1% |
S186S (AGT→AGC) |
dhaL ← |
dihydroxyacetone kinase, C‑terminal domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 801,414 | 0 | A | G | 53.1%
| 7.6
/ 65.0
| 49 | S186S (AGT→AGC) | dhaL | dihydroxyacetone kinase, C‑terminal domain |
| Reads supporting (aligned to +/- strand): ref base A (11/12); new base G (12/14); total (23/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTTGTGCAGCG > minE/801348‑801480
|
tGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACt < 1:640557/69‑1 (MQ=255)
cGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGt < 1:3227647/71‑1 (MQ=255)
cGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGt < 1:1038425/71‑1 (MQ=255)
cGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGt < 1:1426204/71‑1 (MQ=255)
cTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCa > 1:502354/1‑71 (MQ=255)
cGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCga < 1:3312800/71‑1 (MQ=255)
cGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCga < 1:1541288/71‑1 (MQ=255)
cGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCga < 1:308969/71‑1 (MQ=255)
gCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCAcc > 1:1413652/1‑68 (MQ=255)
cAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCgaga > 1:2173700/1‑70 (MQ=255)
aCATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGCCCAATGCTGCGTTCACCGAGATaa > 1:2285922/1‑71 (MQ=255)
aCATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGt > 1:130966/1‑59 (MQ=255)
aCATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATaa < 1:1197867/71‑1 (MQ=255)
cATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGtt < 1:2730717/59‑1 (MQ=255)
tttGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTg > 1:282632/1‑71 (MQ=255)
ttGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATa > 1:3119515/1‑49 (MQ=255)
ttGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTg > 1:2542898/1‑70 (MQ=255)
catAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGc > 1:2657089/1‑71 (MQ=255)
catAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGc > 1:1877207/1‑71 (MQ=255)
aaaCATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCtt > 1:2590730/1‑71 (MQ=255)
aaCATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCttt < 1:2054963/71‑1 (MQ=255)
aaCATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCttt < 1:1372414/71‑1 (MQ=255)
aaCATCACCGAGGTCGCACCGGGATCCTGGTAACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCttt < 1:1387929/71‑1 (MQ=255)
cACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGc < 1:3665787/38‑1 (MQ=255)
cACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGc < 1:110968/38‑1 (MQ=255)
aCCGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGc < 1:1403273/71‑1 (MQ=255)
ccGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCgag < 1:1144559/46‑1 (MQ=255)
ccGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCgag < 1:3598511/46‑1 (MQ=255)
ccGAGGTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCgag < 1:3329800/46‑1 (MQ=255)
cGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCtt < 1:37911/71‑1 (MQ=255)
gTCGCGCCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCat > 1:2793871/1‑71 (MQ=255)
gcgcCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAAc > 1:262879/1‑43 (MQ=255)
gcgcCGGGATCCTGGTGACCAATGCTGCGTTCACCGAGATAAc > 1:2375547/1‑43 (MQ=255)
cGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCatcgtaatc > 1:2097357/1‑71 (MQ=255)
ggATCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCatcgtaatcg > 1:278947/1‑70 (MQ=255)
tCCTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACt < 1:485381/71‑1 (MQ=255)
cTGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACttt < 1:536259/71‑1 (MQ=255)
tGGTGACCAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTtg > 1:2701589/1‑71 (MQ=255)
ggTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCatcg > 1:897953/1‑57 (MQ=255)
ggTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCatc > 1:1994328/1‑56 (MQ=255)
ggTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACt < 1:3546951/67‑1 (MQ=255)
gACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTtgtg > 1:3163649/1‑69 (MQ=255)
gACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTtgtg > 1:3032907/1‑69 (MQ=255)
ccAATGCTGCGTTCACCGAGATAACTGGCGCGGCCttt < 1:680294/38‑1 (MQ=255)
cAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTg > 1:2807847/1‑46 (MQ=255)
cAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTg > 1:384798/1‑46 (MQ=255)
cAATGCTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTTGTGCAGCg > 1:3605620/1‑71 (MQ=255)
cAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTTGTGCAGCg < 1:3060173/71‑1 (MQ=255)
cAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTTGTGCAGCg < 1:2371419/71‑1 (MQ=255)
aaTACTGCGTTCACCGAGATAACTGGCGCGGCCtt < 1:438752/35‑1 (MQ=255)
|
TGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAACTGGCGCGGCCTTTGCGGGCTTGCATCGTAATCGTACTTTGTGCAGCG > minE/801348‑801480
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
