Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,932,996 C→T 30.1% V483I (GTA→ATA) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,932,9960CT30.1% 199.7 / 105.6 143V483I (GTA→ATA) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (63/37);  new base T (30/13);  total (93/50)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 154 total aligned reads displayed.

ATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCT  >  minE/1932927‑1933066
                                                                     |                                                                      
aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                                        <  1:4162378/70‑1 (MQ=255)
aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                                        <  1:1140601/70‑1 (MQ=255)
 ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                      >  1:2969026/1‑71 (MQ=255)
 ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                      >  1:152248/1‑71 (MQ=255)
    tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc                                                                   >  1:4696342/1‑71 (MQ=255)
      ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg                                                                  <  1:469845/70‑1 (MQ=255)
      ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg                                                                  <  1:5661682/70‑1 (MQ=255)
         gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                               >  1:4596429/1‑70 (MQ=255)
               tCGCCGGTAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                                        <  1:2761608/55‑1 (MQ=255)
               tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                                        <  1:5327225/55‑1 (MQ=255)
                cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                                       >  1:214042/1‑71 (MQ=255)
                cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTTTGTAAACAGCATAATCATTCGGt                                                                >  1:4371568/1‑62 (MQ=255)
                cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTT‑GTAAACAGCATAATCATTCGGt                                                                >  1:3371417/1‑61 (MQ=255)
                 gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                                       >  1:1222664/1‑70 (MQ=255)
                 gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                                       >  1:4542885/1‑70 (MQ=255)
                 gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                                       >  1:1549438/1‑70 (MQ=255)
                   cGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                                             >  1:2007391/1‑62 (MQ=255)
                    gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa                                                       >  1:538936/1‑67 (MQ=255)
                    gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                   <  1:1912456/71‑1 (MQ=255)
                     ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                                         <  1:1215495/64‑1 (MQ=255)
                       aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa                                                 <  1:4105807/67‑1 (MQ=255)
                       aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                   >  1:605765/1‑68 (MQ=255)
                         cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                              >  1:818567/1‑71 (MQ=255)
                         cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                              >  1:2554270/1‑71 (MQ=255)
                          aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                               >  1:2029828/1‑53 (MQ=255)
                           gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                            >  1:1768795/1‑71 (MQ=255)
                           gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                            >  1:2071754/1‑71 (MQ=255)
                           gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                            >  1:602986/1‑71 (MQ=255)
                           gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                            >  1:4361469/1‑71 (MQ=255)
                           gAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                                              >  1:591399/1‑69 (MQ=255)
                            aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                                            >  1:1113569/1‑70 (MQ=255)
                            aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAccag                                           >  1:3842704/1‑69 (MQ=255)
                             aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa                                               >  1:4995649/1‑66 (MQ=255)
                                aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                                     <  1:5310255/57‑1 (MQ=255)
                                 cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                                     <  1:5224403/56‑1 (MQ=255)
                                 cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                                     <  1:3724019/56‑1 (MQ=255)
                                  gTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCa                                                        >  1:4889263/1‑52 (MQ=255)
                                   tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                    <  1:5073843/71‑1 (MQ=255)
                                   tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                                         <  1:1028680/50‑1 (MQ=255)
                                   tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                                         <  1:4781095/50‑1 (MQ=255)
                                   tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                                         <  1:1262694/50‑1 (MQ=255)
                                   tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg                                         <  1:65892/66‑2 (MQ=255)
                                   tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg                                         <  1:2874951/66‑2 (MQ=255)
                                    cgcgAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg                                         <  1:4651124/65‑2 (MQ=255)
                                       ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAGTTCCCAGACCTGCTGTcggcgg                                 <  1:2206873/70‑1 (MQ=255)
                                       ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                           <  1:2438293/60‑1 (MQ=255)
                                       ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                                <  1:4035160/71‑1 (MQ=255)
                                        gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                                >  1:561937/1‑70 (MQ=255)
                                        gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                                >  1:1655341/1‑70 (MQ=255)
                                         atagTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtgg                                    >  1:3781893/4‑65 (MQ=255)
                                            gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                            >  1:3022853/1‑70 (MQ=255)
                                             tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg                                        <  1:3806969/57‑3 (MQ=255)
                                                 gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt                      >  1:4266918/1‑71 (MQ=255)
                                                 gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt                      >  1:259517/1‑71 (MQ=255)
                                                  cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                     <  1:2287609/71‑1 (MQ=255)
                                                  cTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg                                        >  1:3006644/1‑50 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTCTCGGCGGTAAGCCATGTTCCa                   <  1:227321/70‑1 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa                               >  1:4117786/1‑58 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa                               >  1:4531051/1‑58 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc                    <  1:2511018/69‑1 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc                    <  1:5648073/69‑1 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc                    <  1:1677124/69‑1 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc                    <  1:498485/69‑1 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg                  >  1:249944/1‑71 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg                  >  1:106959/1‑71 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg                  <  1:1957555/71‑1 (MQ=255)
                                                     ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGAGGTa                               >  1:163794/1‑58 (MQ=255)
                                                       ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                              >  1:639598/1‑41 (MQ=255)
                                                       ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg                >  1:4782943/1‑71 (MQ=255)
                                                       ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg                >  1:3662225/1‑71 (MQ=255)
                                                        gTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg                <  1:4421421/70‑1 (MQ=255)
                                                         tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg              >  1:837025/1‑71 (MQ=255)
                                                         tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg              <  1:594551/71‑1 (MQ=255)
                                                          aaaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag             >  1:3994867/2‑69 (MQ=255)
                                                          aaaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag             >  1:2897954/2‑69 (MQ=255)
                                                           aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc                                              >  1:4576652/1‑37 (MQ=255)
                                                           aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc                                              >  1:4574457/1‑37 (MQ=255)
                                                           aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc                                              >  1:4565460/1‑37 (MQ=255)
                                                           aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt            <  1:244594/71‑1 (MQ=255)
                                                           aaCAGCATAATCATTCGGTCGATACCAATACCCAGa                                               >  1:4090682/1‑36 (MQ=16)
                                                           aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtggcggca                               >  1:2556513/1‑50 (MQ=255)
                                                           aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgca              >  1:3310219/1‑68 (MQ=12)
                                                           aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGt            >  1:1117808/1‑71 (MQ=255)
                                                             cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                         >  1:4860255/1‑56 (MQ=255)
                                                             cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                         >  1:5240619/1‑56 (MQ=255)
                                                              aGCATTACCATACGGTCGATACCAATTCCCAGACct                                            >  1:2953900/1‑36 (MQ=255)
                                                              aGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCAc         >  1:1145874/1‑71 (MQ=255)
                                                               gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg              >  1:3103808/1‑65 (MQ=255)
                                                               gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg              >  1:1454453/1‑65 (MQ=255)
                                                                   tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa     >  1:1038074/1‑70 (MQ=255)
                                                                    aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                   >  1:4662066/1‑55 (MQ=255)
                                                                    aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                   >  1:106668/1‑55 (MQ=255)
                                                                    aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt    <  1:711145/70‑1 (MQ=255)
                                                                    aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc   >  1:4053110/1‑71 (MQ=255)
                                                                    aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc   >  1:449954/1‑71 (MQ=255)
                                                                     ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                   >  1:1317486/1‑54 (MQ=255)
                                                                     ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc   <  1:2725785/70‑1 (MQ=255)
                                                                     ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc   <  1:4487654/70‑1 (MQ=255)
                                                                     ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc   <  1:4741292/70‑1 (MQ=255)
                                                                     ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCt  <  1:3005456/71‑1 (MQ=255)
                                                                     |                                                                      
ATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCT  >  minE/1932927‑1933066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A