Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,932,996 C→T 15.8% V483I (GTA→ATA) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,932,9960CT15.8% 248.4 / 32.2 101V483I (GTA→ATA) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (45/40);  new base T (8/8);  total (53/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 102 total aligned reads displayed.

TTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATC  >  minE/1932928‑1933065
                                                                    |                                                                     
ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAcc                                                                      <  1:1169249/70‑1 (MQ=255)
ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                     >  1:878366/1‑71 (MQ=255)
ttttACCGGACGCATCGCCGCGAACAGCATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                     >  1:2314682/1‑71 (MQ=255)
   tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa                                                                   <  1:1280495/70‑1 (MQ=255)
   tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc                                                                  >  1:2324401/1‑71 (MQ=255)
     ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCGGTTGGTGAACAGCATTACCATACg                                                                 <  1:3049851/70‑1 (MQ=255)
       ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                              <  1:142934/71‑1 (MQ=255)
       ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                              <  1:3554281/71‑1 (MQ=255)
       ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                              <  1:1995375/71‑1 (MQ=255)
        gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                              >  1:2499762/1‑70 (MQ=255)
             aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                     >  1:342351/1‑58 (MQ=255)
             aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc                                                         >  1:1589153/1‑70 (MQ=255)
             aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc                                                         >  1:399179/1‑70 (MQ=255)
                gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                                      >  1:389074/1‑70 (MQ=255)
                 ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATa                                                          <  1:2249842/65‑1 (MQ=255)
                 ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAta                                                    <  1:1387639/71‑2 (MQ=255)
                 ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt                                                     >  1:2309855/1‑70 (MQ=255)
                  cGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAta                                                    <  1:3545116/70‑2 (MQ=255)
                   gggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc                                                  <  1:2036599/71‑1 (MQ=255)
                    ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAt                                                                    <  1:2573551/52‑1 (MQ=255)
                    ggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                                            <  1:957812/60‑1 (MQ=255)
                    ggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                  >  1:2420740/1‑70 (MQ=255)
                     gAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa                                                <  1:3836490/71‑1 (MQ=255)
                     gAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                  >  1:446491/1‑69 (MQ=255)
                     gAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                  >  1:51047/1‑69 (MQ=255)
                      aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                  >  1:2017540/1‑68 (MQ=255)
                       aCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGa                                              >  1:2874504/1‑71 (MQ=255)
                        gaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                                             >  1:2195732/2‑71 (MQ=255)
                           aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa                                                                   >  1:2444327/1‑46 (MQ=255)
                           aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa                                                                   >  1:2073444/1‑46 (MQ=255)
                           aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                          >  1:3221528/1‑71 (MQ=255)
                           aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                          >  1:729160/1‑71 (MQ=255)
                           aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGAATTACCATa                                                                   >  1:2595537/1‑46 (MQ=255)
                           aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAcca                                           >  1:439714/1‑69 (MQ=255)
                            aTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc                                         >  1:265374/1‑71 (MQ=255)
                             tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                        <  1:2236446/71‑1 (MQ=255)
                              aaCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg                                       >  1:881339/1‑71 (MQ=255)
                               aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                                      <  1:2107835/71‑1 (MQ=255)
                               aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                                      <  1:1883498/71‑1 (MQ=255)
                                 gTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                              >  1:3812308/1‑45 (MQ=255)
                                 gTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                        <  1:1849638/67‑1 (MQ=255)
                                  tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                             <  1:1516799/61‑1 (MQ=255)
                                  tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                        <  1:2579557/66‑1 (MQ=255)
                                  tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg                                    >  1:3709333/1‑70 (MQ=255)
                                     cgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg                                <  1:949646/71‑1 (MQ=255)
                                      ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                        <  1:596495/62‑1 (MQ=255)
                                      ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg                                >  1:891360/1‑70 (MQ=255)
                                        atagTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtggcgg                                >  1:3952323/4‑68 (MQ=255)
                                        aTGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                               >  1:622335/1‑69 (MQ=255)
                                         tGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAg                            <  1:3250687/71‑1 (MQ=255)
                                          ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                           >  1:1002513/1‑71 (MQ=255)
                                          ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                           >  1:143374/1‑71 (MQ=255)
                                          ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                           >  1:2167885/1‑71 (MQ=255)
                                             aTGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                   <  1:1840271/60‑1 (MQ=255)
                                             aTGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                   <  1:1447979/60‑1 (MQ=255)
                                              tGGCTGTTGGTGAACAGCATTATCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                       >  1:1916970/1‑71 (MQ=255)
                                              tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                   >  1:2915610/1‑59 (MQ=255)
                                              tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                       >  1:205013/1‑71 (MQ=255)
                                              tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCACAGACCTGCTGTCGGCGGTAAGCCATg                       >  1:1699821/1‑71 (MQ=255)
                                               ggCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                        <  1:2707742/53‑1 (MQ=255)
                                               ggCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                         <  1:1754461/68‑1 (MQ=255)
                                                gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGcc                          >  1:1147443/1‑66 (MQ=255)
                                                gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                      <  1:1736708/70‑1 (MQ=255)
                                                 cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                    <  1:1157779/71‑1 (MQ=255)
                                                 cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                    <  1:1806520/71‑1 (MQ=255)
                                                  tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                        >  1:500213/1‑66 (MQ=255)
                                                  tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                    >  1:608211/1‑70 (MQ=255)
                                                  tGTTGGTGAACAGCATTACCATACCGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                        >  1:389763/1‑66 (MQ=255)
                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTccc                                                 <  1:3230337/39‑1 (MQ=255)
                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTccc                                                 <  1:314631/39‑1 (MQ=255)
                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                  <  1:3740287/70‑1 (MQ=255)
                                                     tGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg                                       <  1:3630332/48‑1 (MQ=255)
                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                  >  1:429589/1‑68 (MQ=255)
                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGt                <  1:610347/70‑1 (MQ=255)
                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg               >  1:1557379/1‑71 (MQ=255)
                                                       gTGAACAGCATTACCATACTGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg               <  1:81702/70‑1 (MQ=255)
                                                       gTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg               <  1:1431549/70‑1 (MQ=255)
                                                        tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg             <  1:126706/71‑1 (MQ=255)
                                                          aaCAGCATAATCATTCGGTCGATACCAATACCCAGAc                                             >  1:2030058/1‑37 (MQ=21)
                                                          aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag            <  1:1518668/70‑3 (MQ=255)
                                                          aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag            <  1:3533722/70‑3 (MQ=255)
                                                           aCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc                                     <  1:86598/44‑1 (MQ=255)
                                                            cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                         >  1:2858227/1‑55 (MQ=255)
                                                             aGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa         <  1:950687/70‑1 (MQ=255)
                                                              gCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACg       >  1:1010793/1‑71 (MQ=255)
                                                               cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg       >  1:448310/1‑70 (MQ=255)
                                                               cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt      <  1:2788785/71‑1 (MQ=255)
                                                                 ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc                   <  1:2087148/56‑1 (MQ=255)
                                                                 ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTg          <  1:836498/65‑1 (MQ=255)
                                                                 ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa    >  1:198306/1‑71 (MQ=255)
                                                                 ttACCATACGGTCGATACCAATTCCCAGACCTGCTCTCGGCGGTAAGCCATg                       <  1:2845356/52‑1 (MQ=255)
                                                                  tACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                 <  1:164329/41‑1 (MQ=255)
                                                                  tACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                 <  1:277304/41‑1 (MQ=255)
                                                                  tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt   >  1:3450901/1‑71 (MQ=255)
                                                                   aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                      >  1:306058/1‑51 (MQ=255)
                                                                   aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                      >  1:2295852/1‑51 (MQ=255)
                                                                   aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                  >  1:3916373/1‑55 (MQ=255)
                                                                   aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt   <  1:3606931/70‑1 (MQ=255)
                                                                   aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc  >  1:427271/1‑71 (MQ=255)
                                                                   aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc  >  1:2344956/1‑71 (MQ=255)
                                                                    |                                                                     
TTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATC  >  minE/1932928‑1933065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A