Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,069 A→G 26.4% D458D (GAT→GAC)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,0690AG26.4% 386.3 / 153.1 235D458D (GAT→GAC) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (102/71);  new base G (38/24);  total (140/95)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGA  >  minE/1932999‑1933138
                                                                      |                                                                     
tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTGAGCCATGTTCCAGTGCGGTGACGTAATCTTCa                                                                       >  1:4605625/1‑71 (MQ=255)
tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa                                                                       >  1:3330490/1‑71 (MQ=255)
tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa                                                                       <  1:4850848/71‑1 (MQ=255)
tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa                                                                       >  1:4409699/1‑71 (MQ=255)
  cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc                                                                     >  1:2249762/1‑71 (MQ=255)
  cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc                                                                     >  1:3019300/1‑71 (MQ=255)
     tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                                                                    >  1:2556433/1‑69 (MQ=255)
     tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                                                                    >  1:5486413/1‑69 (MQ=255)
     tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                                                                    >  1:3344681/1‑69 (MQ=255)
      cGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                                                                    >  1:4360687/1‑68 (MQ=255)
       gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTa                                                                  >  1:177889/1‑69 (MQ=255)
       gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                                                                 >  1:344547/1‑70 (MQ=255)
       gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                                                                 <  1:4864166/70‑1 (MQ=255)
       gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                                                                 >  1:585365/1‑70 (MQ=255)
        aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa                                                               <  1:2874446/71‑1 (MQ=255)
          aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa                                                                       >  1:5337729/1‑61 (MQ=255)
          aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                                                                      >  1:2142107/1‑62 (MQ=255)
           ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa                                                                <  1:203293/67‑1 (MQ=255)
           ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                                                             <  1:5325187/70‑1 (MQ=255)
           ccAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACa                                                             <  1:2339575/70‑1 (MQ=255)
            cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                           >  1:5018567/1‑71 (MQ=255)
              aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc                                                         >  1:2187071/1‑71 (MQ=255)
               ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                           <  1:203776/68‑1 (MQ=255)
                tcacAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt                                                       >  1:227026/4‑71 (MQ=255)
                 cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                      >  1:1049344/1‑71 (MQ=255)
                  ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg                                                     >  1:4437206/1‑71 (MQ=255)
                  ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                      <  1:867354/70‑1 (MQ=255)
                   cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                                                                      >  1:679348/1‑53 (MQ=255)
                   cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                                                                      >  1:4010927/1‑53 (MQ=255)
                    aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc                                                   <  1:642669/71‑1 (MQ=255)
                    aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc                                                   >  1:1584344/1‑71 (MQ=255)
                    aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc                                                   >  1:2688439/1‑71 (MQ=255)
                    aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc                                                   >  1:3930457/1‑71 (MQ=255)
                    aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgt                                                    >  1:4269045/1‑70 (MQ=255)
                       ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc                                                <  1:857411/71‑1 (MQ=255)
                        ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa                                               <  1:3368009/71‑1 (MQ=255)
                         tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                              >  1:3667897/1‑71 (MQ=255)
                           ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt                                            >  1:1489893/1‑71 (MQ=255)
                             gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc                                                   >  1:4159028/1‑62 (MQ=255)
                                ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt                                                    >  1:5431395/1‑58 (MQ=255)
                                ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCCTCGTCGTCACCTGCGTc                                       >  1:2235179/1‑71 (MQ=255)
                                    gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                      <  1:1561213/52‑1 (MQ=255)
                                    gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                   <  1:3262295/71‑1 (MQ=255)
                                     tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                  <  1:5427428/71‑1 (MQ=255)
                                      aaGCCAAGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc                                 >  1:2210741/1‑71 (MQ=255)
                                       aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                              >  1:5115191/1‑57 (MQ=255)
                                       aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                              >  1:3049866/1‑57 (MQ=255)
                                       aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                              >  1:2749332/1‑57 (MQ=255)
                                       aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                >  1:1326127/1‑71 (MQ=255)
                                         ccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtc                                                      <  1:1729618/47‑1 (MQ=255)
                                          cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc                                                   >  1:2885321/1‑49 (MQ=255)
                                          cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                              >  1:2967475/1‑70 (MQ=255)
                                            tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                      <  1:3339026/60‑1 (MQ=255)
                                              ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt                         >  1:3531122/1‑71 (MQ=255)
                                              ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACa                                                             >  1:3405306/1‑35 (MQ=12)
                                              ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                  >  1:1858649/1‑60 (MQ=255)
                                              ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                  >  1:3308526/1‑60 (MQ=255)
                                               tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                           >  1:3686878/1‑36 (MQ=255)
                                               tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                           >  1:3768674/1‑36 (MQ=255)
                                                ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa                       >  1:369527/1‑71 (MQ=255)
                                                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                 >  1:5569178/1‑61 (MQ=255)
                                                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                       >  1:1089950/1‑70 (MQ=255)
                                                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                       >  1:3222538/1‑70 (MQ=255)
                                                 cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCAc                                              >  1:5659632/1‑47 (MQ=255)
                                                  aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                  >  1:2415427/1‑58 (MQ=255)
                                                   gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                     >  1:476922/1‑70 (MQ=255)
                                                    tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt                                    <  1:1386820/54‑1 (MQ=255)
                                                    tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt                                    <  1:1154704/54‑1 (MQ=255)
                                                        gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                    <  1:702811/66‑1 (MQ=255)
                                                        gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                    <  1:1990504/66‑1 (MQ=255)
                                                          caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCttt                                    <  1:4309067/47‑1 (MQ=255)
                                                          caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac             >  1:3999744/2‑69 (MQ=255)
                                                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGcag                                        >  1:1134714/1‑41 (MQ=37)
                                                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg            <  1:2627342/71‑1 (MQ=255)
                                                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg            >  1:5455186/1‑71 (MQ=255)
                                                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg            >  1:108465/1‑71 (MQ=255)
                                                           aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                        <  1:140950/59‑1 (MQ=255)
                                                            cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                 <  1:4942817/49‑1 (MQ=255)
                                                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                  >  1:551934/1‑45 (MQ=255)
                                                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                >  1:3202087/1‑48 (MQ=19)
                                                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                >  1:5189370/1‑48 (MQ=19)
                                                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                >  1:4627129/1‑48 (MQ=19)
                                                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg            <  1:251110/69‑1 (MQ=255)
                                                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt          >  1:2153822/1‑71 (MQ=255)
                                                             gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc             <  1:2108966/68‑1 (MQ=255)
                                                             gTAATCTTAGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                >  1:361452/1‑48 (MQ=16)
                                                               aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa                             <  1:1507352/50‑1 (MQ=255)
                                                               aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt           <  1:2769100/68‑1 (MQ=255)
                                                                aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg       >  1:5262763/1‑71 (MQ=255)
                                                                aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc        <  1:2946673/70‑1 (MQ=255)
                                                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                >  1:4352900/1‑43 (MQ=255)
                                                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                >  1:3642964/1‑43 (MQ=255)
                                                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                >  1:1303626/1‑43 (MQ=255)
                                                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc     >  1:1685781/1‑70 (MQ=255)
                                                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt    >  1:2063233/1‑71 (MQ=255)
                                                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt    >  1:2486038/1‑71 (MQ=255)
                                                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                              <  1:1238746/43‑1 (MQ=255)
                                                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt    <  1:5634821/69‑1 (MQ=255)
                                                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg   <  1:3157432/70‑1 (MQ=255)
                                                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa  >  1:728693/1‑71 (MQ=255)
                                                                      |                                                                     
TACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGA  >  minE/1932999‑1933138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A