Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,933,069 |
A→G |
12.1% |
D458D (GAT→GAC) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,933,069 | 0 | A | G | 12.1%
| 306.8
/ 27.8
| 116 | D458D (GAT→GAC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (51/51); new base G (7/7); total (58/58) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 |
Only 100 of 122 total aligned reads displayed.
TACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAT > minE/1932999‑1933139
|
tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa < 1:221560/71‑1 (MQ=255)
cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt < 1:2233645/70‑1 (MQ=255)
ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:3665241/71‑1 (MQ=255)
ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:2583459/71‑1 (MQ=255)
gTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:3580744/1‑70 (MQ=255)
tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:227480/1‑69 (MQ=255)
gATACCAATTCCCAGACTTGCTGTCGGCTGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa < 1:3717702/71‑1 (MQ=255)
gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:3441203/67‑1 (MQ=255)
gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:508248/1‑70 (MQ=255)
gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:1807213/1‑70 (MQ=255)
gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:1471501/1‑70 (MQ=255)
aTACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa < 1:458509/71‑1 (MQ=255)
aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:132951/71‑1 (MQ=255)
aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:549750/71‑1 (MQ=255)
tACAAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa > 1:3182211/1‑69 (MQ=255)
aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc > 1:2634888/1‑70 (MQ=255)
aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:3843692/1‑71 (MQ=255)
ccAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACAt > 1:3047784/1‑71 (MQ=255)
atacccAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGc > 1:2026012/4‑71 (MQ=255)
aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:14951/1‑71 (MQ=255)
aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2469833/1‑71 (MQ=255)
aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:548675/1‑71 (MQ=255)
aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2041472/1‑71 (MQ=255)
ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt > 1:2388482/1‑71 (MQ=255)
ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:1395080/71‑1 (MQ=255)
ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:3455080/71‑1 (MQ=255)
ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:923091/71‑1 (MQ=255)
ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:1022371/71‑1 (MQ=255)
ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:1395967/71‑1 (MQ=255)
aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:23360/1‑52 (MQ=255)
aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:1968959/1‑71 (MQ=255)
aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:606744/1‑71 (MQ=255)
aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:3440995/1‑71 (MQ=255)
aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:1605980/1‑71 (MQ=255)
aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:2100237/71‑1 (MQ=255)
ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:1069018/71‑1 (MQ=255)
ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt > 1:3359588/1‑51 (MQ=255)
ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:1392547/53‑1 (MQ=255)
tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt > 1:1142805/1‑50 (MQ=255)
tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt > 1:3108360/1‑50 (MQ=255)
tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt > 1:2228414/1‑61 (MQ=255)
tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:491385/1‑66 (MQ=255)
tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:1406876/1‑66 (MQ=255)
gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc > 1:101896/1‑71 (MQ=255)
ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc > 1:3646882/1‑53 (MQ=255)
ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt < 1:3801730/71‑1 (MQ=255)
ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt < 1:2406103/71‑1 (MQ=255)
tgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc < 1:794000/68‑1 (MQ=255)
tgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg > 1:3416936/1‑71 (MQ=255)
tgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg > 1:1639521/1‑71 (MQ=255)
tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa < 1:1932364/41‑1 (MQ=255)
tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa > 1:249419/1‑48 (MQ=255)
tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa > 1:244793/1‑48 (MQ=255)
tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa > 1:2602037/1‑48 (MQ=255)
tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGc < 1:1749900/70‑1 (MQ=255)
tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg < 1:773795/71‑1 (MQ=255)
ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGt > 1:2236883/1‑70 (MQ=255)
gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTa < 1:2612694/43‑1 (MQ=255)
gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCg < 1:1780924/51‑1 (MQ=255)
gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCg < 1:636197/51‑1 (MQ=255)
gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc < 1:401731/63‑1 (MQ=255)
gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc < 1:792217/63‑1 (MQ=255)
gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc < 1:1933875/70‑1 (MQ=255)
cggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCtt > 1:2751356/1‑71 (MQ=255)
ggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt < 1:1822936/71‑1 (MQ=255)
gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt < 1:1668258/36‑1 (MQ=255)
gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:3943209/71‑1 (MQ=255)
gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:371382/71‑1 (MQ=255)
aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc > 1:465693/1‑71 (MQ=255)
gaccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca < 1:3431786/69‑2 (MQ=255)
gaccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca < 1:3303413/69‑2 (MQ=255)
aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt < 1:3384045/55‑1 (MQ=255)
aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc > 1:1187514/1‑69 (MQ=255)
tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:655513/47‑1 (MQ=255)
tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc > 1:462003/1‑71 (MQ=255)
ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACATGCAGCTTTagcatt > 1:3003156/1‑63 (MQ=255)
ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:2700032/56‑1 (MQ=255)
ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa > 1:1081700/1‑71 (MQ=255)
cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCAcc < 1:2691026/48‑1 (MQ=255)
aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc < 1:746536/71‑1 (MQ=255)
tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:563076/1‑69 (MQ=255)
aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCtt < 1:192207/46‑1 (MQ=255)
aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc < 1:2875832/70‑1 (MQ=255)
aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg < 1:1330031/71‑1 (MQ=255)
cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt < 1:2198952/71‑1 (MQ=255)
gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGAt > 1:1103000/1‑59 (MQ=255)
gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGAt > 1:2021538/1‑59 (MQ=255)
gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGtt < 1:2438942/71‑1 (MQ=255)
tAATCTTCATCGTAGAACATCGCTTCTTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg < 1:2984257/68‑1 (MQ=255)
tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg < 1:906911/48‑1 (MQ=255)
tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg < 1:1175115/68‑1 (MQ=255)
aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGAt > 1:2283533/1‑57 (MQ=255)
aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc > 1:2933978/1‑71 (MQ=255)
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg > 1:3277849/1‑71 (MQ=255)
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc < 1:3570258/70‑1 (MQ=255)
ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcc > 1:3735240/1‑55 (MQ=255)
cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa > 1:646527/1‑71 (MQ=255)
aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg > 1:379081/1‑60 (MQ=255)
aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa < 1:1915100/70‑1 (MQ=255)
aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt > 1:384461/1‑71 (MQ=255)
|
TACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAT > minE/1932999‑1933139
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
