Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,100 T→G 43.0% D448A (GAC→GCC) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1000TG43.0% 5.6 / 234.6 170D448A (GAC→GCC) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (45/52);  new base G (38/35);  total (83/87)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 173 total aligned reads displayed.

GGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGT  >  minE/1933031‑1933169
                                                                     |                                                                     
 gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                                     <  1:4702600/71‑1 (MQ=255)
 gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                                     <  1:132381/71‑1 (MQ=255)
    gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                                                  <  1:4853767/71‑1 (MQ=255)
    gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                                                  <  1:3262295/71‑1 (MQ=255)
     tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                                                 <  1:5427428/71‑1 (MQ=255)
      aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc                                                                >  1:5192428/1‑71 (MQ=255)
      aaGCCAAGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc                                                                >  1:2210741/1‑71 (MQ=255)
         ccATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCt                                                                     <  1:1116696/63‑1 (MQ=255)
           aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGAGTCTTTCGCGGCaa                                                           >  1:226970/1‑71 (MQ=255)
            tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                                     <  1:3339026/60‑1 (MQ=255)
            tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                               <  1:3178810/66‑1 (MQ=255)
            tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                               <  1:4510530/66‑1 (MQ=255)
            tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                               <  1:3206314/66‑1 (MQ=255)
              ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt                                                        >  1:3531122/1‑71 (MQ=255)
              ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                                                 >  1:1858649/1‑60 (MQ=255)
               tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg                                                       >  1:2664724/1‑71 (MQ=255)
                ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa                                                      >  1:369527/1‑71 (MQ=255)
                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                                                >  1:2511602/1‑61 (MQ=255)
                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                      >  1:3222538/1‑70 (MQ=255)
                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                      >  1:1089950/1‑70 (MQ=255)
                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                      >  1:1492064/1‑70 (MQ=255)
                ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                      >  1:4727515/1‑70 (MQ=255)
                 cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                     <  1:691635/71‑3 (MQ=255)
                 cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                     <  1:2131891/71‑3 (MQ=255)
                  aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                                                 >  1:2415427/1‑58 (MQ=255)
                   gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                    >  1:476922/1‑70 (MQ=255)
                   gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                    >  1:2973139/1‑70 (MQ=255)
                    tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt                                                                   <  1:1154704/54‑1 (MQ=255)
                    tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                                     <  1:4422532/52‑1 (MQ=255)
                    tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                    >  1:1347867/1‑69 (MQ=255)
                        gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                   <  1:208561/66‑1 (MQ=255)
                        gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                   <  1:1990504/66‑1 (MQ=255)
                        gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                   <  1:702811/66‑1 (MQ=255)
                          caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac                                            >  1:3999744/2‑69 (MQ=255)
                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                                                 >  1:5537666/1‑47 (MQ=255)
                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcctg                                                 >  1:1652409/1‑63 (MQ=255)
                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                           <  1:2627342/71‑1 (MQ=255)
                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                           >  1:5455186/1‑71 (MQ=255)
                           aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                           >  1:108465/1‑71 (MQ=255)
                           aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                       <  1:140950/59‑1 (MQ=255)
                           aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                            <  1:4591973/70‑1 (MQ=255)
                           aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                            <  1:5499635/70‑1 (MQ=255)
                            cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                                                <  1:4942817/49‑1 (MQ=255)
                            cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                          <  1:344419/71‑1 (MQ=255)
                            cGTAATCTTCAACGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAcc                                                         <  1:835081/56‑1 (MQ=255)
                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                               >  1:4627129/1‑48 (MQ=19)
                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                               >  1:5189370/1‑48 (MQ=19)
                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                           <  1:251110/69‑1 (MQ=255)
                             gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                         <  1:2052306/71‑1 (MQ=255)
                             gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                            <  1:5420870/68‑1 (MQ=255)
                             gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                            <  1:2108966/68‑1 (MQ=255)
                             gTAATCTTAGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                               >  1:361452/1‑48 (MQ=16)
                              tAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGttc                                        <  1:145909/71‑2 (MQ=255)
                               aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                          <  1:2769100/68‑1 (MQ=255)
                                aTCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                               >  1:2153076/1‑45 (MQ=16)
                                aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCacc                                                          >  1:22391/1‑49 (MQ=255)
                                aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                       <  1:2946673/70‑1 (MQ=255)
                                aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                       <  1:1217528/70‑1 (MQ=255)
                                aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCTGATCCAGGAAGCGTTgcg                                      >  1:3048664/1‑71 (MQ=255)
                                 tCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                       <  1:3514704/69‑1 (MQ=255)
                                  cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc                                                                      >  1:2245386/1‑37 (MQ=255)
                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                               >  1:1303626/1‑43 (MQ=255)
                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                               >  1:4230971/1‑43 (MQ=255)
                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                                                 >  1:4335824/1‑41 (MQ=255)
                                   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                    >  1:1685781/1‑70 (MQ=255)
                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                     <  1:3314824/67‑1 (MQ=255)
                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                   <  1:5634821/69‑1 (MQ=255)
                                     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg                                  <  1:3157432/70‑1 (MQ=255)
                                       tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATc                               <  1:2769368/68‑1 (MQ=255)
                                         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                          <  1:557120/57‑1 (MQ=255)
                                         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                             >  1:504192/2‑71 (MQ=255)
                                         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                              >  1:3321430/2‑70 (MQ=255)
                                         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                              <  1:1578500/69‑1 (MQ=255)
                                          tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                         <  1:3810730/58‑1 (MQ=255)
                                          tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                         <  1:1038443/58‑1 (MQ=255)
                                          tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc                            <  1:5379538/71‑1 (MQ=255)
                                            gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                          >  1:5693059/1‑69 (MQ=255)
                                            gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                           <  1:4171349/70‑2 (MQ=255)
                                            gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                          >  1:4037584/1‑71 (MQ=255)
                                             aaCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcgc                         <  1:2846754/71‑1 (MQ=255)
                                               cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                       >  1:2141943/1‑71 (MQ=255)
                                               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                    <  1:5451451/58‑1 (MQ=255)
                                               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                    <  1:2982565/58‑1 (MQ=255)
                                               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                    <  1:3622461/58‑1 (MQ=255)
                                                 tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                     <  1:4602582/69‑2 (MQ=255)
                                                 tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                     <  1:304919/69‑2 (MQ=255)
                                                 tCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCt                                                        >  1:4109142/1‑36 (MQ=255)
                                                  ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                     <  1:567082/69‑2 (MQ=255)
                                                  cGCTTCGTCGTCACCTGCGTCTTTCGCTGCAACCTGATCCAGGAAGc                                            <  1:1793986/47‑1 (MQ=255)
                                                   gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                   >  1:5288223/1‑71 (MQ=255)
                                                    cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGtt                                         <  1:51440/48‑1 (MQ=255)
                                                      tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                  <  1:102543/69‑2 (MQ=255)
                                                       cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                <  1:185405/70‑4 (MQ=255)
                                                       cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                <  1:5573518/70‑4 (MQ=255)
                                                       cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttta                 <  1:1583054/69‑3 (MQ=255)
                                                        gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                >  1:3920543/1‑66 (MQ=255)
                                                         tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc             >  1:2887221/1‑71 (MQ=255)
                                                           gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAg                                             <  1:2196866/37‑1 (MQ=255)
                                                            tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt          <  1:5443102/71‑1 (MQ=255)
                                                                   cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg   >  1:3763480/1‑71 (MQ=255)
                                                                     |                                                                     
GGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGT  >  minE/1933031‑1933169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A