Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,105 C→A 43.1% A446A (GCG→GCT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1050CA43.1% 109.3 / 134.8 132A446A (GCG→GCT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (37/38);  new base A (25/32);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 163 total aligned reads displayed.

GTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACC  >  minE/1933035‑1933173
                                                                      |                                                                    
gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                                                      <  1:3262295/71‑1 (MQ=255)
gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                                                      <  1:4807766/71‑1 (MQ=255)
 tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                                                     <  1:5427428/71‑1 (MQ=255)
  aaGCCAAGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc                                                                    >  1:2210741/1‑71 (MQ=255)
     ccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagcatt                                                                 <  1:2202938/71‑4 (MQ=255)
      cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                                                                 >  1:2967475/1‑70 (MQ=255)
       aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGAGTCTTTCGCGGCaa                                                               >  1:226970/1‑71 (MQ=255)
        tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                   <  1:4510530/66‑1 (MQ=255)
        tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                   <  1:3178810/66‑1 (MQ=255)
          ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt                                                            >  1:3531122/1‑71 (MQ=255)
           tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg                                                           >  1:2664724/1‑71 (MQ=255)
            ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                                                    >  1:5569178/1‑61 (MQ=255)
            ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                          >  1:1492064/1‑70 (MQ=255)
            ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                          >  1:4727515/1‑70 (MQ=255)
            ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                          >  1:3222538/1‑70 (MQ=255)
             cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                         <  1:2131888/71‑3 (MQ=255)
             cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                         <  1:691635/71‑3 (MQ=255)
                tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                        >  1:1347867/1‑69 (MQ=255)
                    gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                       <  1:702811/66‑1 (MQ=255)
                    gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                       <  1:208561/66‑1 (MQ=255)
                    gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAgg                                                    >  1:2885049/1‑69 (MQ=255)
                      caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac                                                >  1:3999744/2‑69 (MQ=255)
                       aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcctg                                                     >  1:1652409/1‑63 (MQ=255)
                       aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                               >  1:5455186/1‑71 (MQ=255)
                       aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                               >  1:108465/1‑71 (MQ=255)
                       aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                           <  1:140950/59‑1 (MQ=255)
                       aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                           <  1:1086110/59‑1 (MQ=255)
                        cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                                                    <  1:4942817/49‑1 (MQ=255)
                        cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                              <  1:344419/71‑1 (MQ=255)
                        cGTAATCTTCAACGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAcc                                                             <  1:835081/56‑1 (MQ=255)
                         gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                                   >  1:5189370/1‑48 (MQ=19)
                         gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                                   >  1:3202087/1‑48 (MQ=19)
                         gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                                   >  1:4627129/1‑48 (MQ=19)
                         gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                               <  1:251110/69‑1 (MQ=255)
                         gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                             <  1:1957255/71‑1 (MQ=255)
                         gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                             >  1:2153822/1‑71 (MQ=255)
                         gTAATCTTAGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                                                   >  1:361452/1‑48 (MQ=16)
                          tAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGttc                                            <  1:145909/71‑2 (MQ=255)
                            aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                          >  1:5262763/1‑71 (MQ=255)
                            aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                           <  1:2946673/70‑1 (MQ=255)
                            aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                           <  1:1217528/70‑1 (MQ=255)
                            aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCTGATCCAGGAAGCGTTgcg                                          >  1:3048664/1‑71 (MQ=255)
                             tCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                                                     <  1:2562381/43‑1 (MQ=255)
                             tCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                           <  1:3514704/69‑1 (MQ=255)
                              cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                       >  1:1760708/1‑56 (MQ=255)
                              cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGa                                                   >  1:401019/1‑60 (MQ=255)
                               ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaa                                                 >  1:3260059/1‑60 (MQ=255)
                               ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGa                                                   <  1:5156722/59‑1 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                   >  1:5443595/1‑43 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                   >  1:1303626/1‑43 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                   >  1:3642964/1‑43 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                   >  1:1201384/1‑43 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                                                     >  1:4335824/1‑41 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                         >  1:5430545/1‑69 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                        >  1:1685781/1‑70 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                       >  1:2063233/1‑71 (MQ=255)
                               ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                       >  1:2486038/1‑71 (MQ=255)
                                   tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATc                                   <  1:2769368/68‑1 (MQ=255)
                                    catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                  >  1:3431730/3‑71 (MQ=255)
                                     atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                              <  1:4595056/57‑1 (MQ=255)
                                     atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                              <  1:557120/57‑1 (MQ=255)
                                     atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                 >  1:504192/2‑71 (MQ=255)
                                     atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                 >  1:4084607/2‑71 (MQ=255)
                                     atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                  <  1:1578500/69‑1 (MQ=255)
                                     atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                  >  1:3321430/2‑70 (MQ=255)
                                      tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                             <  1:3810730/58‑1 (MQ=255)
                                      tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                             <  1:1038443/58‑1 (MQ=255)
                                      tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc                                <  1:5379538/71‑1 (MQ=255)
                                        gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                              >  1:3853750/1‑69 (MQ=255)
                                        gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                               <  1:4171349/70‑2 (MQ=255)
                                           cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                           >  1:2141943/1‑71 (MQ=255)
                                           cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                           <  1:4430440/71‑1 (MQ=255)
                                           cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTACTGGAAACGTTCAGCCTGATCTTCTGCGt                           >  1:2382617/1‑71 (MQ=255)
                                           cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                              >  1:3133129/1‑52 (MQ=255)
                                           cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                        <  1:4142501/58‑1 (MQ=255)
                                           cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                        <  1:3622461/58‑1 (MQ=255)
                                             tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                         <  1:4602582/69‑2 (MQ=255)
                                             tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                         <  1:5685779/69‑2 (MQ=255)
                                             tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                         <  1:304919/69‑2 (MQ=255)
                                              cGCTTCGTCGTCACCTGCGTCTTTCGCTGCAACCTGATCCAGGAAGc                                                <  1:1793986/47‑1 (MQ=255)
                                               gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                       >  1:5288223/1‑71 (MQ=255)
                                               gCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                 >  1:2250125/1‑61 (MQ=255)
                                                cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt                                    >  1:2837605/1‑57 (MQ=255)
                                                cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                      <  1:2975721/71‑1 (MQ=255)
                                                  tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                      <  1:102543/69‑2 (MQ=255)
                                                   cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                    <  1:185405/70‑4 (MQ=255)
                                                   cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttta                     <  1:1583054/69‑3 (MQ=255)
                                                    gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                    >  1:2656606/1‑66 (MQ=255)
                                                     tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                 <  1:5644894/71‑1 (MQ=255)
                                                      cgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc                                <  1:2014241/55‑1 (MQ=255)
                                                       gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAg                                                 <  1:4729331/37‑1 (MQ=255)
                                                       gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc               >  1:5584112/1‑71 (MQ=255)
                                                            cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg          >  1:2133253/1‑71 (MQ=255)
                                                               cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg       >  1:3763480/1‑71 (MQ=255)
                                                                 tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt      >  1:5461608/1‑70 (MQ=255)
                                                                  cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa    >  1:3019898/1‑71 (MQ=255)
                                                                   tttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc   >  1:1110760/1‑71 (MQ=255)
                                                                    ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc         >  1:4955044/1‑64 (MQ=255)
                                                                     tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc   <  1:3032305/69‑1 (MQ=255)
                                                                      cgcgGCAACCTGATCCAGGACGCGTTGCGCCTGATc                                   >  1:5638580/1‑36 (MQ=255)
                                                                      |                                                                    
GTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACC  >  minE/1933035‑1933173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A