Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,933,240 |
C→T |
53.7% |
A401A (GCG→GCA) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,933,240 | 0 | C | T | 53.7%
| ‑0.1
/ 118.6
| 95 | A401A (GCG→GCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (16/28); new base T (22/29); total (38/57) |
| Fisher's exact test for biased strand distribution p-value = 5.35e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01 |
Only 100 of 101 total aligned reads displayed.
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAC > minE/1933171‑1933308
|
aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg > 1:1279968/1‑71 (MQ=255)
aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:4415099/71‑1 (MQ=255)
aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5034452/71‑1 (MQ=255)
aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg > 1:610317/1‑71 (MQ=255)
aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5182046/71‑1 (MQ=255)
tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:114508/71‑1 (MQ=255)
tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:4345069/71‑1 (MQ=255)
tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:2494489/71‑1 (MQ=255)
tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:1902831/71‑1 (MQ=255)
tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGcc > 1:3604451/1‑66 (MQ=255)
tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg < 1:2327593/70‑1 (MQ=255)
tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg < 1:5217669/70‑1 (MQ=255)
cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1954216/69‑1 (MQ=255)
cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGGCAGCGgag > 1:4861846/1‑70 (MQ=255)
cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag > 1:2485440/1‑70 (MQ=255)
gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCTTGCCAGCGgggac < 1:4978718/71‑2 (MQ=255)
ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc > 1:1928819/1‑71 (MQ=255)
ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc > 1:1245625/1‑71 (MQ=255)
accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa < 1:5295457/67‑1 (MQ=255)
gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg < 1:5022301/70‑1 (MQ=255)
actGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:5183383/68‑1 (MQ=255)
aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:5497285/1‑55 (MQ=255)
aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:3819135/1‑55 (MQ=255)
aTGAAGAATTCAAAACGGGCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGc < 1:162148/71‑1 (MQ=255)
tGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:3702050/68‑1 (MQ=255)
gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:1030113/63‑1 (MQ=255)
gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:3077125/63‑1 (MQ=255)
aagaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg < 1:4965131/70‑1 (MQ=255)
aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg < 1:3482038/71‑1 (MQ=255)
aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg > 1:2914009/1‑70 (MQ=255)
agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:4604216/61‑1 (MQ=255)
gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa > 1:2674907/1‑70 (MQ=255)
ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5411983/47‑1 (MQ=19)
ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:1416983/47‑1 (MQ=19)
ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:2235573/47‑1 (MQ=19)
tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg > 1:4740757/1‑64 (MQ=255)
tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg > 1:2266241/1‑64 (MQ=255)
tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTc > 1:5697636/1‑71 (MQ=255)
cAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt > 1:4524972/1‑69 (MQ=255)
aaaaCGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTccg > 1:4684276/1‑69 (MQ=255)
aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa > 1:1058383/1‑64 (MQ=255)
aacggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTa < 1:2695751/69‑1 (MQ=255)
aacggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTa < 1:5613074/69‑1 (MQ=255)
aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:4494449/71‑1 (MQ=255)
aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:5057228/71‑1 (MQ=255)
gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:766922/1‑39 (MQ=255)
gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:1776076/1‑39 (MQ=255)
gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:2978820/1‑39 (MQ=255)
gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTaa > 1:2623724/1‑71 (MQ=255)
cggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:3392334/41‑1 (MQ=19)
cggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga > 1:3525716/1‑47 (MQ=19)
cGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAAt < 1:2902075/71‑1 (MQ=255)
ggTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGata > 1:3379239/1‑61 (MQ=255)
tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1272112/38‑1 (MQ=255)
tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:5655235/38‑1 (MQ=255)
tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt < 1:3115275/47‑1 (MQ=255)
ggtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa > 1:546466/2‑55 (MQ=255)
gtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:4739304/1‑71 (MQ=255)
gtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGcc < 1:4504936/51‑1 (MQ=255)
gtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGcc < 1:3685336/51‑1 (MQ=255)
tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGatat < 1:2685242/56‑1 (MQ=255)
tgATTTCCGGGTTAACGTCGTTACGACGCGCAAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:4597413/1‑71 (MQ=255)
tgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt < 1:310383/71‑2 (MQ=255)
aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt > 1:1742510/1‑43 (MQ=255)
ccGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:645268/37‑1 (MQ=255)
cGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGaa > 1:239370/1‑71 (MQ=255)
gggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa < 1:2620717/69‑1 (MQ=255)
gggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa < 1:3645299/69‑1 (MQ=255)
gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:5534655/1‑69 (MQ=255)
gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:4157015/71‑1 (MQ=255)
gTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:5486266/71‑1 (MQ=255)
ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:5676097/71‑1 (MQ=255)
ttAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAg < 1:2243293/71‑1 (MQ=255)
aaCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa < 1:1599594/70‑1 (MQ=255)
aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:5617751/1‑65 (MQ=255)
aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:1530850/1‑65 (MQ=255)
aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt > 1:4055516/1‑71 (MQ=255)
cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:2597515/50‑1 (MQ=255)
cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:3767432/50‑1 (MQ=255)
tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGa < 1:3596984/36‑1 (MQ=255)
tcattACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGa > 1:5360930/4‑66 (MQ=255)
tcattACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAtg < 1:2922824/65‑1 (MQ=255)
gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:3358170/71‑1 (MQ=255)
gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:2449454/1‑71 (MQ=255)
attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:580606/2‑49 (MQ=19)
attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:4785117/2‑49 (MQ=19)
attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt > 1:2834892/2‑51 (MQ=19)
attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt > 1:4030066/2‑51 (MQ=19)
attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt < 1:187248/70‑1 (MQ=255)
ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATaaa < 1:2350342/47‑1 (MQ=19)
ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg < 1:3570228/52‑3 (MQ=19)
ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTc > 1:2088010/1‑71 (MQ=255)
tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:4351435/60‑1 (MQ=255)
tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:40475/60‑1 (MQ=255)
acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg > 1:2387713/1‑48 (MQ=19)
acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg > 1:638248/1‑48 (MQ=19)
acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTg > 1:3141325/1‑71 (MQ=255)
gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc < 1:3442001/71‑1 (MQ=255)
cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc < 1:2829183/71‑1 (MQ=255)
gcgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt < 1:5121648/52‑1 (MQ=255)
|
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAC > minE/1933171‑1933308
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
