Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,933,240 |
C→T |
12.5% |
A401A (GCG→GCA) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,933,240 | 0 | C | T | 12.5%
| 128.4
/ 8.6
| 48 | A401A (GCG→GCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (17/25); new base T (2/4); total (19/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAC > minE/1933171‑1933308
|
aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc < 1:1211217/70‑1 (MQ=255)
cGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:797113/1‑70 (MQ=255)
aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:1621589/1‑71 (MQ=255)
aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg < 1:3030350/71‑1 (MQ=255)
aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg < 1:3266250/71‑1 (MQ=255)
tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:3372925/1‑67 (MQ=255)
tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg < 1:2259486/70‑1 (MQ=255)
tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:413429/71‑1 (MQ=255)
tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:638612/70‑1 (MQ=255)
cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:1015024/1‑65 (MQ=255)
cGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga > 1:2921249/1‑71 (MQ=255)
cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:700539/71‑1 (MQ=255)
gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:3321789/70‑1 (MQ=255)
gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa < 1:2394561/71‑1 (MQ=255)
accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaaa > 1:1221087/1‑71 (MQ=255)
accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa < 1:1241886/70‑1 (MQ=255)
caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAATCt < 1:273048/71‑1 (MQ=255)
caATGAAGCACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt > 1:2549195/1‑71 (MQ=255)
agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt < 1:2271152/63‑1 (MQ=255)
gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc > 1:1784565/1‑46 (MQ=255)
gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc > 1:2138673/1‑46 (MQ=255)
gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc > 1:1741593/1‑46 (MQ=255)
aCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata < 1:525533/70‑1 (MQ=255)
cAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt > 1:1097369/1‑69 (MQ=255)
aaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:471292/53‑1 (MQ=255)
aaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:466910/53‑1 (MQ=255)
aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt > 1:2519784/1‑54 (MQ=255)
aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt > 1:1379386/1‑54 (MQ=255)
cGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAAt < 1:3339145/71‑1 (MQ=255)
gTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa < 1:704248/71‑1 (MQ=255)
tCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCa < 1:2701564/63‑1 (MQ=255)
aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTaa < 1:73438/61‑1 (MQ=255)
aTTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg > 1:554603/1‑68 (MQ=255)
tCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt > 1:3422507/1‑70 (MQ=255)
ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg < 1:5978/70‑1 (MQ=255)
ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg < 1:1139038/70‑1 (MQ=255)
ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg < 1:1115944/70‑1 (MQ=255)
cGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGaa > 1:2494362/1‑71 (MQ=255)
cGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGa < 1:862675/70‑1 (MQ=255)
ggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:3521032/1‑70 (MQ=255)
gTTATCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:301384/71‑1 (MQ=255)
gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg > 1:927109/1‑41 (MQ=255)
ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:451700/51‑1 (MQ=255)
ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa < 1:3208918/66‑1 (MQ=255)
aCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt < 1:1666789/70‑1 (MQ=255)
gtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg > 1:741130/1‑71 (MQ=255)
tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:2809988/45‑1 (MQ=255)
tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc > 1:3219514/1‑71 (MQ=255)
tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc > 1:2874648/1‑71 (MQ=255)
tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTCAATCAGATGTGc > 1:2118866/1‑71 (MQ=255)
acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:2891770/68‑1 (MQ=255)
acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:1248812/68‑1 (MQ=255)
acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTg > 1:1541410/1‑71 (MQ=255)
cgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt > 1:2138303/1‑52 (MQ=255)
cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc > 1:1590119/1‑71 (MQ=255)
gcgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGc < 1:1110939/67‑1 (MQ=255)
cgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTg < 1:1172822/48‑1 (MQ=255)
|
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAC > minE/1933171‑1933308
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
