Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,155,529	A→G	35.3%	intergenic (+40/+146)	dacB → / ← obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,155,529	0	A	G	35.3%	46.4 / 27.9	34	intergenic (+40/+146)	dacB/obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand): ref base A (12/10); new base G (2/10); total (14/20)
Fisher's exact test for biased strand distribution p-value = 6.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

TTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTG  >  minE/2155459‑2155595

tttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatta                                                                    <  1:2278123/71‑1 (MQ=255)

       aaaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                                              >  1:2133692/1‑70 (MQ=255)

                tATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                     <  1:2648301/70‑1 (MQ=255)

                                 aaaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                   >  1:2760005/1‑71 (MQ=255)

                                  aaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                  >  1:146625/1‑71 (MQ=255)

                                   aaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                 >  1:2048622/1‑71 (MQ=255)

                                      gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt                               >  1:837092/1‑70 (MQ=255)

                                      gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                              <  1:3823536/71‑1 (MQ=255)

                                              ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt                       >  1:4772423/1‑70 (MQ=255)

                                                 acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGca                                          >  1:4035108/1‑47 (MQ=255)

                                                 acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                   >  1:4800180/1‑71 (MQ=255)

                                                 acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                   >  1:3364822/1‑71 (MQ=255)

                                                  caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                                <  1:3530875/41‑1 (MQ=255)

                                                  caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                                <  1:3065056/41‑1 (MQ=255)

                                                  caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                              <  1:1301565/59‑1 (MQ=255)

                                                    tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                              <  1:2977235/57‑1 (MQ=255)

                                                    tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                              <  1:2921788/57‑1 (MQ=255)

                                                    tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                              <  1:1927413/57‑1 (MQ=255)

                                                      gCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc               <  1:4255506/70‑1 (MQ=255)

                                                          cggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc           >  1:3673691/2‑70 (MQ=11)

                                                           ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa         <  1:1553601/71‑1 (MQ=11)

                                                           ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa          >  1:3050819/1‑70 (MQ=11)

                                                           ggCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa          >  1:5520019/1‑70 (MQ=255)

                                                             cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa       <  1:3245084/71‑1 (MQ=11)

                                                             cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa       <  1:3841057/71‑1 (MQ=11)

                                                             cTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa       <  1:3285390/71‑1 (MQ=255)

                                                              tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                    <  1:255958/39‑1 (MQ=14)

                                                              tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                    <  1:4175532/39‑1 (MQ=14)

                                                              tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                    <  1:5139261/39‑1 (MQ=14)

                                                              tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                       <  1:3603031/52‑1 (MQ=11)

                                                              tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa       <  1:1356754/68‑1 (MQ=11)

                                                              tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa       <  1:4236095/68‑1 (MQ=11)

                                                               acAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                    <  1:5145935/39‑1 (MQ=14)

                                                                cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg    >  1:4382492/1‑71 (MQ=255)

                                                                 aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                            >  1:1573383/1‑46 (MQ=255)

                                                                   attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                              <  1:5410303/42‑2 (MQ=255)

                                                                   attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                              <  1:864957/42‑2 (MQ=255)

                                                                   attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg  >  1:474442/1‑70 (MQ=255)

                                                                   attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCAGCGtt                              <  1:1713143/42‑2 (MQ=11)

TTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTG  >  minE/2155459‑2155595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A