Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R2	251	90.0	5200563	86.9%	4519289	59.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,155,529	A→G	14.3%	intergenic (+40/+146)	dacB → / ← obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,155,529	0	A	G	14.3%	87.3 / 6.9	35	intergenic (+40/+146)	dacB/obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand): ref base A (14/16); new base G (3/2); total (17/18)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAA  >  minE/2155461‑2155599

tGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat                                                                       <  1:3468451/70‑1 (MQ=255)

  tataAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTg                                                                     <  1:2947863/70‑1 (MQ=255)

  tataAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTg                                                                     <  1:5105805/70‑1 (MQ=255)

      aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaaa                                                                <  1:3609270/71‑1 (MQ=255)

                tCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt                                                       <  1:3591502/70‑1 (MQ=255)

                         ttAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGt                                                              >  1:4284690/1‑54 (MQ=255)

                         ttAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTa                                                  >  1:437690/1‑66 (MQ=255)

                         ttAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCg                                             <  1:411054/71‑1 (MQ=255)

                            gTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGa                                           <  1:5029606/70‑1 (MQ=255)

                                 aaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                     >  1:1094493/1‑71 (MQ=255)

                                    gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                     <  1:407887/52‑1 (MQ=255)

                                       aCCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                                 <  1:697930/37‑1 (MQ=255)

                                           cGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt                           >  1:735555/1‑71 (MQ=255)

                                           cGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt                           >  1:4380898/1‑71 (MQ=255)

                                              cacaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt                                                        >  1:2301868/1‑39 (MQ=255)

                                              cacaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt                                                        >  1:1930754/1‑39 (MQ=255)

                                                   ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg                    >  1:1751575/1‑70 (MQ=255)

                                                   ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                   >  1:841965/1‑71 (MQ=255)

                                                   ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAAAGCCTTATCCGGc                   >  1:222517/1‑71 (MQ=255)

                                                   ggCCGCGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                 >  1:4171027/1‑57 (MQ=255)

                                                            tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                 <  1:4326983/62‑1 (MQ=11)

                                                            tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                 <  1:4278650/62‑1 (MQ=11)

                                                             tCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGcc                            >  1:1863045/1‑52 (MQ=255)

                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc               >  1:4166163/1‑64 (MQ=255)

                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc               >  1:3280805/1‑64 (MQ=255)

                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc               >  1:2267739/1‑64 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                                >  1:2148579/1‑46 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                                >  1:3370355/1‑46 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:763949/44‑1 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:656312/44‑1 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:893721/44‑1 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:982521/44‑1 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:2551119/44‑1 (MQ=255)

                                                               aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:296044/44‑1 (MQ=255)

                                                                gATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:3902879/43‑1 (MQ=255)

                                                                gATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:2477515/43‑1 (MQ=255)

                                                                 attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:1085459/42‑1 (MQ=255)

                                                                 attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:4628730/42‑1 (MQ=255)

                                                                 attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:2408081/42‑1 (MQ=255)

                                                                 attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:777712/42‑1 (MQ=255)

                                                                 attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:1468123/42‑1 (MQ=255)

                                                                 attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                  <  1:4047696/42‑1 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:2119871/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:2209558/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:1879137/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:869525/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:24202/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:5063918/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:3842137/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:2540806/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:2768067/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:3133601/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:4130201/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                    >  1:3455003/1‑39 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAt          >  1:4585261/1‑65 (MQ=255)

                                                                  ttattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc     >  1:4591056/1‑70 (MQ=255)

                                                                   tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt                                   <  1:4136959/39‑1 (MQ=255)

                                                                   tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                                >  1:3627590/1‑42 (MQ=255)

                                                                   tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa    >  1:2172985/1‑70 (MQ=255)

                                                                   tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa    >  1:3840130/1‑70 (MQ=255)

                                                                    attGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa  >  1:2696007/1‑71 (MQ=255)

                                                                    attGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa  >  1:3062189/1‑71 (MQ=255)

TGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAA  >  minE/2155461‑2155599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A