Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,872,347 |
A→G |
K83K (AAA→AAG) |
yeaI → |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,872,347 | 0 | A | G | 100.0%
| 24.5
/ NA
| 9 | K83K (AAA→AAG) | yeaI | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/0); total (9/0) |
CACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTC > W3110S.gb/1872335‑1872395
|
gacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:1647227/2‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:1028428/1‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:1448280/1‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:1550848/1‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:2261029/1‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:2687847/1‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:277184/1‑61 (MQ=255)
cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:554475/1‑61 (MQ=255)
cacaAATGTAAAGCTAATCCCACAGATGAATTACCTGATGgttgtt > 1:10844/1‑46 (MQ=255)
|
CACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTC > W3110S.gb/1872335‑1872395
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
