Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,857,513 |
C→T |
G58E (GGA→GAA) |
ydjG ← |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,857,513 | 0 | C | T | 100.0%
| 156.7
/ NA
| 47 | G58E (GGA→GAA) | ydjG | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/19); total (28/19) |
CGGGGCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATC > W3110S.gb/1857456‑1857568
|
cGGGGCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTgg > 1:2875551/1‑62 (MQ=255)
gCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCa < 1:3144780/62‑1 (MQ=255)
cAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAg > 1:1127341/1‑62 (MQ=255)
cAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAg > 1:4364274/1‑62 (MQ=255)
tttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTatcaa < 1:1056390/61‑1 (MQ=255)
tttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTatcaa < 1:4364427/61‑1 (MQ=255)
tttAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTatcaa < 1:675154/61‑1 (MQ=255)
cGCCTGCCCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAg > 1:2991562/1‑60 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAg > 1:4244626/1‑60 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAg > 1:3002829/1‑60 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAg > 1:763889/1‑60 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAg > 1:3379119/1‑60 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa > 1:3016428/1‑61 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa > 1:4547066/1‑61 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa > 1:1917468/1‑61 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa > 1:612728/1‑61 (MQ=255)
cGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa > 1:3401000/1‑61 (MQ=255)
cGCCTGAACGACGATAACTTCACTATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGa > 1:2362836/1‑61 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:4131412/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:903430/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:3944107/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:580010/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:558924/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:2406389/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:1100092/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:1124617/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:1406868/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:1522035/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:1571259/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:2005766/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:2680526/35‑1 (MQ=255)
aaCTTCACTATTGCCAAAGTTATATTCTGGCGCAg < 1:2932535/35‑1 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:4097079/1‑35 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:3976717/1‑35 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:4194992/1‑35 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:1890072/1‑35 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:1612865/1‑35 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:37519/1‑35 (MQ=255)
cTATTGCCAAAGTTATATTCTGGCGCAGTatcaat > 1:2930607/1‑35 (MQ=255)
tATTGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCa < 1:405574/62‑1 (MQ=255)
tGCCAAAGTTATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGa > 1:300277/1‑62 (MQ=255)
ttATATTCTGGCGCAGTATCAATCAGATTAATGCCAGAACGATGGGCTTCAAGAATCGTATc > 1:801288/1‑62 (MQ=255)
ttATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATc > 1:2248690/1‑62 (MQ=255)
ttATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATc > 1:2700935/1‑62 (MQ=255)
ttATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATc > 1:3586068/1‑62 (MQ=255)
ttATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATc > 1:3492798/1‑62 (MQ=255)
ttATATTCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATc > 1:880430/1‑62 (MQ=255)
|
CGGGGCAGTTTTTTTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATC > W3110S.gb/1857456‑1857568
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
