Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,208,632 |
T→C |
L235L (TTG→CTG) |
yehL → |
predicted transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,208,632 | 0 | T | C | 86.1%
| 106.7
/ 9.0
| 43 | L235L (TTG→CTG) | yehL | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base C (37/0); total (37/6) |
| Fisher's exact test for biased strand distribution p-value = 1.64e-07 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
TTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATT > W3110S.gb/2208587‑2208682
|
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:395810/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2531887/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1266094/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2754374/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2793098/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2905239/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2953371/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:3050393/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:334917/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:367589/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2540743/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:553420/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:684169/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:685455/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:699042/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:752707/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:799556/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:860991/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:924642/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:957454/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2287068/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1545842/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2251459/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:158365/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2124127/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1600536/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2041489/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2016077/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1768400/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2369525/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:181571/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1796579/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg > 1:461007/1‑48 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg > 1:1379311/1‑48 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg > 1:2133963/1‑48 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTAAGGATTTCGCCCAGGAGCTgg > 1:2765893/1‑49 (MQ=37)
ttCGATTTTGAAACTGTCTTT‑CGATTATGGATTTCGCCCAGGAGCTgg > 1:2308705/1‑48 (MQ=25)
aTTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGccc < 1:1699817/61‑1 (MQ=255)
ggAGTTGGAACTGGTTGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTAtt < 1:1977420/55‑1 (MQ=255)
ggAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTAtt < 1:628653/55‑1 (MQ=255)
ggAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTAtt < 1:1612616/55‑1 (MQ=255)
ggAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTAtt < 1:2123198/55‑1 (MQ=255)
gAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTAtt < 1:650609/54‑1 (MQ=255)
|
TTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATT > W3110S.gb/2208587‑2208682
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
