Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R2
|
24 |
47.7 |
2431847 |
96.1% |
2337004 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
817,564 |
C→T |
L113L (CTC→CTT) |
prfA → |
peptide chain release factor RF‑1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 817,564 | 0 | C | T | 95.7%
| 140.9
/ ‑1.2
| 47 | L113L (CTC→CTT) | prfA | peptide chain release factor RF‑1 |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (23/22); total (24/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 |
CAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGC > minE/817496‑817633
|
cAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGa < 1:968161/71‑1 (MQ=255)
cAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGa < 1:1538685/71‑1 (MQ=255)
cTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGt > 1:21663/1‑71 (MQ=255)
gAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc < 1:832632/69‑1 (MQ=255)
aTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGaa > 1:556705/1‑71 (MQ=255)
aTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGaa > 1:229676/1‑71 (MQ=255)
ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc < 1:2322269/71‑1 (MQ=255)
ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc > 1:711993/1‑71 (MQ=255)
ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc > 1:1714820/1‑71 (MQ=255)
ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGaa < 1:831734/57‑1 (MQ=255)
aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAcc > 1:956262/1‑70 (MQ=255)
aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:2419422/1‑71 (MQ=255)
aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:2208722/1‑71 (MQ=255)
aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:1205817/1‑71 (MQ=255)
gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGcc > 1:1825407/1‑60 (MQ=255)
gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcgg < 1:791117/68‑1 (MQ=255)
gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg < 1:1323525/67‑1 (MQ=255)
ttCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCggcggc < 1:696453/71‑1 (MQ=255)
gTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc < 1:579702/49‑1 (MQ=255)
gTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc < 1:1254546/49‑1 (MQ=255)
ttACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGcgacga < 1:949029/71‑1 (MQ=255)
aCTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGaa > 1:626153/1‑70 (MQ=255)
cTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAg < 1:243170/70‑1 (MQ=255)
gCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGt < 1:1556713/42‑1 (MQ=255)
ccAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc > 1:1794142/1‑53 (MQ=255)
aaaGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcgg < 1:1787157/67‑1 (MQ=255)
aGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:1227636/1‑50 (MQ=255)
gATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc > 1:2049883/1‑36 (MQ=255)
gATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc > 1:1395202/1‑36 (MQ=255)
cTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgcg < 1:2406752/71‑1 (MQ=255)
cTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgc < 1:1971296/70‑1 (MQ=255)
atgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcggc > 1:2105485/1‑44 (MQ=255)
atgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc > 1:1777498/1‑71 (MQ=255)
tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCgg < 1:2341330/68‑1 (MQ=255)
tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc > 1:1831853/1‑70 (MQ=255)
tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCg < 1:658994/71‑1 (MQ=255)
aCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCg < 1:1697700/69‑1 (MQ=255)
aCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGa < 1:1768541/70‑1 (MQ=255)
cGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCt < 1:1096892/57‑1 (MQ=255)
aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:1786985/1‑35 (MQ=255)
aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCggg > 1:2287539/1‑63 (MQ=255)
aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTg < 1:1107964/70‑1 (MQ=255)
aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGAGGGCGATCTGt > 1:2310319/1‑71 (MQ=255)
aCGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGt < 1:644907/71‑1 (MQ=255)
cGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTa > 1:624184/1‑71 (MQ=255)
ccTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTa > 1:1566258/1‑69 (MQ=255)
tCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGc > 1:1057033/1‑71 (MQ=255)
|
CAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGC > minE/817496‑817633
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
