Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I1 R2
|
57 |
53.4 |
717274 |
8.4% |
60251 |
54.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,420,657 |
C→T |
T155T (ACC→ACT) |
pta → |
phosphate acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,420,657 | 0 | C | T | 99.7%
| 776.9
/ NA
| 377 | T155T (ACC→ACT) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (0/376); total (0/377) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
Only 100 of 377 total aligned reads displayed.
TTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGACCCGCAACA > W3110S.gb/2420602‑2420664
|
tATTTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:388192/62‑1 (MQ=255)
tATGTCTCTGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATAGAACTGACTCGCAACa < 1:71636/62‑1 (MQ=255)
tATGTCTCAGGTCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:456597/62‑1 (MQ=255)
tATGTCTCAGGGCACTGTTACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:433481/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGACCTGACTCGCAACa < 1:220460/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAATTGACTCGCAACa < 1:600543/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCATCa < 1:682474/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:484004/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:573487/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:567783/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:557408/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:55178/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:549369/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:540400/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:531968/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:506906/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:503737/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:490905/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:580128/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:47765/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:458511/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:440196/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:42455/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:102911/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:416485/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:404992/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:656050/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:9190/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:83355/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:7773/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:712972/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:707575/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:699869/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:695197/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:679481/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:664025/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:657711/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:576778/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:646299/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:637867/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:62413/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:616024/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:608039/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:595038/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:588717/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:422846/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:578633/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:189916/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:402229/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:270863/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:26325/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:253826/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:245285/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:243279/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:235261/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:224575/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:206632/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:199550/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:196779/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:194205/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:27225/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:18232/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:1677/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:160761/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:155177/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:148922/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:144835/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:142031/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:134796/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:125984/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:116419/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:108905/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:285209/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:34957/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:345394/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:339501/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:333810/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:32546/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:355521/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:362778/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:3770/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:386737/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:323097/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:316608/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:39796/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:313342/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:307090/62‑1 (MQ=255)
tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACCCGCAACa < 1:666167/62‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:446416/61‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:407850/61‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:514079/61‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:519644/61‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:525590/61‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:544642/61‑1 (MQ=255)
aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:297656/61‑1 (MQ=255)
tcAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:218497/56‑1 (MQ=255)
gCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:630132/51‑1 (MQ=255)
ggAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:360681/38‑1 (MQ=37)
ggAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:43093/38‑1 (MQ=37)
aCAGTTGAAAGAGCGTATCGAACTGCCTCGCAACa < 1:371267/35‑1 (MQ=25)
|
TTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGACCCGCAACA > W3110S.gb/2420602‑2420664
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
