Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F21 I0 R1
|
1009 |
73.8 |
4146436 |
87.4% |
3623985 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,391,735 |
T→C |
24.7% |
K370E (AAA→GAA) |
fruA ← |
fused fructose‑specific PTS enzyme IIB'BC components |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,391,735 | 0 | T | C | 24.7%
| 128.0
/ 46.4
| 73 | K370E (AAA→GAA) | fruA | fused fructose‑specific PTS enzyme IIB'BC components |
| Reads supporting (aligned to +/- strand): ref base T (26/29); new base C (7/11); total (33/40) |
| Fisher's exact test for biased strand distribution p-value = 5.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 |
GAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTT > minE/1391666‑1391804
|
gAACCTCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTa < 1:3080924/71‑1 (MQ=255)
ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTAcc > 1:3699283/1‑70 (MQ=255)
ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg < 1:384437/71‑1 (MQ=255)
ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCg < 1:443106/71‑1 (MQ=255)
cGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGa < 1:1428275/71‑1 (MQ=255)
ttCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCTCCGATCAg < 1:1052977/71‑1 (MQ=255)
tCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg > 1:3176837/1‑71 (MQ=255)
cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGAt > 1:167157/1‑66 (MQ=255)
cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg > 1:1370503/1‑70 (MQ=255)
gCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTa < 1:1146796/71‑1 (MQ=255)
cAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTa < 1:1939825/70‑1 (MQ=255)
aGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGa < 1:204521/71‑1 (MQ=255)
gTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGat < 1:164556/71‑1 (MQ=255)
tCCCCATGGGCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGatc < 1:926662/71‑1 (MQ=255)
cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg < 1:598093/57‑1 (MQ=255)
cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGatcat < 1:205375/71‑1 (MQ=255)
aTGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGc > 1:3647111/1‑71 (MQ=255)
gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATAGc > 1:496278/1‑68 (MQ=255)
gTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGc > 1:2494588/1‑68 (MQ=255)
tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga > 1:2779581/1‑71 (MQ=255)
tCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga > 1:1117237/1‑71 (MQ=255)
cTGCGGCCAGTGAGTCAGCCCTTCGAGAATGCCCGCAACTGGTTCACCGATCAGGTAGATCATCGCCAgac > 1:3817218/1‑71 (MQ=255)
tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatc < 1:2101642/60‑1 (MQ=255)
cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTa > 1:3670439/1‑43 (MQ=255)
cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGt < 1:3427440/53‑1 (MQ=255)
aGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAgaccgac < 1:4002423/71‑1 (MQ=255)
ccAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGacca > 1:3290093/1‑71 (MQ=255)
ccAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGacca > 1:3285513/1‑71 (MQ=255)
ccAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGacca > 1:2967843/1‑71 (MQ=255)
gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGAccacca < 1:1398400/71‑1 (MQ=255)
gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGAccacca < 1:619701/71‑1 (MQ=255)
gTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGAccacca < 1:3187719/71‑1 (MQ=255)
tGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac > 1:2595212/1‑68 (MQ=255)
gAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat > 1:1838699/1‑49 (MQ=255)
gAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGat > 1:3189556/1‑49 (MQ=255)
gAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGa > 1:3169146/1‑48 (MQ=255)
aGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa < 1:2900723/71‑1 (MQ=255)
aGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTgg > 1:2625078/1‑70 (MQ=255)
aGCCCTTCGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa < 1:2373791/71‑1 (MQ=255)
gCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGaa < 1:1637135/71‑1 (MQ=255)
cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTa < 1:3597245/37‑1 (MQ=255)
cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAg < 1:2617439/38‑1 (MQ=255)
cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAgaccgac < 1:1841050/55‑1 (MQ=255)
cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGaa < 1:308102/68‑1 (MQ=255)
cTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAAtt < 1:1933753/71‑1 (MQ=255)
tCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGa < 1:3265583/37‑1 (MQ=255)
tCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg > 1:3061802/1‑71 (MQ=255)
cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGaaa > 1:1826567/1‑66 (MQ=255)
cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg > 1:2138020/1‑70 (MQ=255)
cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc > 1:1561371/1‑71 (MQ=255)
cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc > 1:2984885/1‑71 (MQ=255)
cGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGc > 1:1553748/1‑71 (MQ=255)
cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGatcat > 1:598690/1‑40 (MQ=255)
cGAGAATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAg < 1:337157/70‑1 (MQ=255)
agaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg < 1:1055288/71‑1 (MQ=255)
agaATGCCAGCAACTGGTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCgg < 1:4143893/71‑1 (MQ=255)
gaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAgaccgac > 1:2940641/1‑49 (MQ=255)
gaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac < 1:2139990/52‑1 (MQ=255)
aTGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAAt > 1:475509/1‑71 (MQ=255)
tGCCAGCAACTGGTTTACCTATCAGGTAGATCATCGCCAgaccgac < 1:3737732/46‑1 (MQ=255)
tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGc < 1:2013622/37‑1 (MQ=255)
tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac < 1:2990845/49‑1 (MQ=255)
tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac < 1:467238/49‑1 (MQ=255)
tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTgg > 1:781664/1‑57 (MQ=255)
gCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCa > 1:1691233/1‑38 (MQ=255)
ccAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGAt > 1:2943981/1‑71 (MQ=255)
ccAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGAt > 1:1391918/1‑71 (MQ=255)
cAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATc < 1:2576145/71‑1 (MQ=255)
cTGGTTTACCGATCAGGTAGATCATCGCCAgaccg < 1:348640/35‑1 (MQ=255)
tGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAgg < 1:2025912/67‑1 (MQ=255)
gTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGt > 1:3851760/1‑71 (MQ=255)
gTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCg < 1:4065136/69‑1 (MQ=255)
gTTCACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCg < 1:2181933/69‑1 (MQ=255)
tACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGttt < 1:1499428/70‑1 (MQ=255)
|
GAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTT > minE/1391666‑1391804
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
