Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I2 R2	42	122.2	1899532	52.7%	1001053	56.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,211,696	G→A	A461V (GCG→GTG)	rng ←	ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,211,696	0	G	A	100.0%	123.1 / NA	38	A461V (GCG→GTG)	rng	ribonuclease G
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/25); total (13/25)

TTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCT > minE/2211656‑2211734

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGTCTCTTCGc                   <  1:36354/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1085958/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:994489/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:989336/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:952803/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:793964/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:705402/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:464437/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:378066/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:289506/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:213882/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1899303/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1844423/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1736568/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1552586/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1182083/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1428165/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1040738/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1297715/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1104901/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1188067/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1637190/62‑1 (MQ=255)

ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1272769/62‑1 (MQ=255)

 tGTACTTTAACCTGTTTGCCAACTAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:83745/61‑1 (MQ=255)

 tGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGc                   <  1:1285647/61‑1 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCACGGCTTCAGCt  >  1:974184/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCa     >  1:1842887/1‑52 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1126522/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:474128/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:939420/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:994832/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:889282/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:753015/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1248157/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1279584/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:253222/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1410096/1‑55 (MQ=255)

                        gAAAATTTCCACTTCCACCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCt  >  1:1722299/1‑55 (MQ=255)

TTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCT > minE/2211656‑2211734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A