Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I2 R2
|
42 |
122.2 |
1899532 |
52.7% |
1001053 |
56.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,345,189 |
C→A |
intergenic (+445/+6) |
glnG → / ← hemN |
fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)/coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,345,189 | 0 | C | A | 100.0%
| 10.6
/ NA
| 5 | intergenic (+445/+6) | glnG/hemN | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)/coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) |
CTCAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAA > minE/2345137‑2345198
|
ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1081627/62‑1 (MQ=255)
ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1250288/62‑1 (MQ=255)
ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1673360/62‑1 (MQ=255)
ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1720659/62‑1 (MQ=255)
ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1743658/62‑1 (MQ=255)
|
CTCAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAA > minE/2345137‑2345198
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
