Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I2 R2
|
42 |
122.2 |
1899532 |
52.7% |
1001053 |
56.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,658,121 |
(TCA)3→2 |
coding (960‑962/1506 nt) |
glpD ← |
sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,658,115 | 0 | T | . | 100.0%
| 20.2
/ NA
| 6 | coding (968/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (6/0); total (6/0) |
| * | minE | 2,658,116 | 0 | C | . | 100.0%
| 20.2
/ NA
| 6 | coding (967/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (6/0); total (6/0) |
| * | minE | 2,658,117 | 0 | A | . | 100.0%
| 20.2
/ NA
| 6 | coding (966/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (6/0); total (6/0) |
GCGAGTCGGACTCATCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACGG > minE/2658104‑2658168
|||
gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCa > 1:1239427/1‑46 (MQ=255)
gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:176001/1‑62 (MQ=255)
gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:176504/1‑62 (MQ=255)
gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:240773/1‑62 (MQ=255)
gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACgg > 1:314775/1‑62 (MQ=255)
gcgAGTCGGAC‑‑‑TCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACg > 1:135587/1‑61 (MQ=255)
|||
GCGAGTCGGACTCATCATCACACAGCGGACGCACACCGGAGTAGGTCCAGACGATATCGTCACGG > minE/2658104‑2658168
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
