Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 354 41.7 3110465 93.1% 2895842 61.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,103,846 T→C Y5H (TAC→CAC)  ompR → DNA‑binding response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,103,8460TC100.0% 483.1 / NA 128Y5H (TAC→CAC) ompRDNA‑binding response regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/73);  total (55/73)

Only 100 of 128 total aligned reads displayed.

CTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACG  >  W3110S.gb/4103798‑4103898
                                                |                                                    
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGAt                                               >  1:2854217/1‑56 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTc                                             >  1:2840084/1‑58 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgt                                       >  1:2402525/1‑63 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2879133/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2059575/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2140317/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2176204/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2293651/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2303952/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2479249/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2537559/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2541563/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2598188/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2623300/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2639983/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2655088/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:257998/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2883140/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2902072/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2946418/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:3074992/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:3096497/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:317952/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:491596/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:541159/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:581700/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:657005/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:694825/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:784865/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:910614/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:912859/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1423517/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1047561/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1074771/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1307645/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1372828/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:2051637/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1527041/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1610117/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1610929/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1757532/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:1792004/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg                                       >  1:191163/1‑64 (MQ=255)
cTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAAACACAAGATTCtggtgg                                       >  1:2493112/1‑64 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1305327/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:374459/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:332065/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:204746/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1250986/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:595867/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:2997017/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:614517/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:12359/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1423122/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1915594/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:66721/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1519170/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:711627/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:240265/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:887579/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:2520374/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1699932/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:1800488/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:863784/64‑1 (MQ=255)
      aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGGCGATg                                 <  1:108702/64‑1 (MQ=255)
       aTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:2482600/63‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:2797165/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:1298126/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:1895249/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:2975517/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:2913972/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:670173/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:2859475/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:2625490/64‑1 (MQ=255)
                aaCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCCATGACGACATgcg                       <  1:2797707/64‑1 (MQ=255)
                   cTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATg                                 <  1:751257/51‑1 (MQ=255)
                             acaaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATgcg                       <  1:2597349/51‑1 (MQ=255)
                                   aaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc   <  1:862930/65‑1 (MQ=255)
                                   aaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc   <  1:2469252/65‑1 (MQ=255)
                                   aaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc   <  1:29879/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:1495781/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:206676/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:2136810/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:1808769/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:101275/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:2581266/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:801892/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:2363993/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:2319530/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:1219990/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:464999/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:1451751/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:2976273/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:1422731/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:572913/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:56742/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:549488/65‑1 (MQ=255)
                                    aTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:1996171/65‑1 (MQ=255)
                                       cAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:2315395/62‑1 (MQ=255)
                                       cAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACg  <  1:3078625/62‑1 (MQ=255)
                                                |                                                    
CTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACG  >  W3110S.gb/4103798‑4103898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A