Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,464,055 |
A→T |
22.4% |
intergenic (+62/‑180) |
pmbA → / → cybC |
predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,464,055 | 0 | A | T | 22.4%
| 97.0
/ 25.1
| 49 | intergenic (+62/‑180) | pmbA/cybC | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
| Reads supporting (aligned to +/- strand): ref base A (22/16); new base T (6/5); total (28/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463985‑4464123
|
ggACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACGCAAAATCATTCGCACTGCa > 1:3482955/1‑71 (MQ=255)
ggACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCa < 1:1069378/71‑1 (MQ=255)
cAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTc > 1:1805779/1‑70 (MQ=255)
cAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc > 1:2535018/1‑70 (MQ=255)
cAGTAAAAAAACTGGCGCGACATGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc > 1:3030306/1‑70 (MQ=255)
gTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc > 1:67818/1‑68 (MQ=255)
tAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCgaga > 1:3029174/1‑71 (MQ=255)
tAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCgaga > 1:391410/1‑71 (MQ=255)
aaaaCTGGCGCGACCTGCCACGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGa < 1:2706649/71‑1 (MQ=255)
aaCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGa > 1:799823/1‑69 (MQ=255)
aCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACg < 1:3460029/66‑1 (MQ=255)
gACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:3364580/1‑71 (MQ=255)
cTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:2214918/62‑1 (MQ=255)
gCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCg > 1:4437136/1‑71 (MQ=255)
cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGc > 1:1338068/1‑71 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:801282/1‑70 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3471404/1‑54 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc > 1:2939152/1‑69 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:4079410/1‑70 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGGACTGCTTCgaga > 1:336580/1‑42 (MQ=38)
taAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:3147579/69‑1 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGa < 1:4535935/46‑1 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:2695392/51‑1 (MQ=255)
aaaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTAc > 1:2612859/1‑71 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCga > 1:54912/1‑38 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:787630/1‑51 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3413300/1‑51 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3089511/1‑51 (MQ=255)
tAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:3305593/1‑70 (MQ=255)
aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:4459808/1‑47 (MQ=255)
aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:605858/40‑1 (MQ=255)
aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt > 1:557630/1‑47 (MQ=255)
aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc < 1:3174645/69‑1 (MQ=255)
aaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGt < 1:3328805/71‑1 (MQ=255)
aaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGt < 1:3841443/71‑1 (MQ=255)
aaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa > 1:2440883/1‑43 (MQ=255)
ttACACAAAATCATTCTCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:1445216/71‑1 (MQ=255)
ttACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:492209/71‑1 (MQ=255)
aTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa > 1:2241478/1‑50 (MQ=255)
aTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTc > 1:1465758/1‑71 (MQ=255)
aTTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGa < 1:1718445/70‑1 (MQ=255)
ttCGCACTGCTTCGAGACGGTAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:3368836/71‑1 (MQ=255)
ttCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:4258942/71‑1 (MQ=255)
ttCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:4293236/71‑1 (MQ=255)
ttCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:4374478/71‑1 (MQ=255)
gCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGa < 1:949597/66‑1 (MQ=255)
cTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCg > 1:3481702/1‑70 (MQ=255)
tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa < 1:1110574/39‑1 (MQ=255)
tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTa < 1:4436322/48‑1 (MQ=255)
gCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAg > 1:860525/1‑71 (MQ=255)
cATCGAGACGGAAAGTGAATGAGGGCC‑GGGATCGTACTCGc < 1:932743/41‑1 (MQ=37)
|
GGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463985‑4464123
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
