Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,464,055 |
A→T |
25.0% |
intergenic (+62/‑180) |
pmbA → / → cybC |
predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,464,055 | 0 | A | T | 25.0%
| 105.8
/ 40.0
| 60 | intergenic (+62/‑180) | pmbA/cybC | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
| Reads supporting (aligned to +/- strand): ref base A (26/19); new base T (9/6); total (35/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 |
AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463992‑4464123
|
aaaaaaaCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGAc > 1:2213020/1‑71 (MQ=255)
aaaaaaCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACg > 1:369641/1‑71 (MQ=255)
gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:3174909/69‑1 (MQ=255)
gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:157788/69‑1 (MQ=255)
gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:3297742/69‑1 (MQ=255)
gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:33129/69‑1 (MQ=255)
cgACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt > 1:1821577/1‑69 (MQ=255)
cgACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt > 1:4051346/1‑69 (MQ=255)
ccTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCga < 1:2255143/52‑1 (MQ=255)
cTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGa < 1:2687178/63‑1 (MQ=255)
gCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa > 1:3485289/1‑61 (MQ=255)
gcgcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg < 1:3588189/71‑1 (MQ=255)
gcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAg > 1:2415497/1‑53 (MQ=255)
gcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAg < 1:2437494/71‑1 (MQ=255)
cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:606900/54‑1 (MQ=255)
cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:897289/54‑1 (MQ=255)
cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:3440253/54‑1 (MQ=255)
ataaaataaaAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAggg > 1:3049661/1‑60 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCgaga > 1:2720589/1‑43 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:282117/1‑58 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:3046939/1‑58 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:3658847/1‑58 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:701354/1‑58 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:2567370/1‑70 (MQ=255)
ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:2909777/1‑70 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGCACTGCTTc < 1:1504320/38‑1 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGCACTGCTTc < 1:1489694/38‑1 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTg < 1:1893739/51‑1 (MQ=255)
taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:2261040/69‑1 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATg > 1:1138494/1‑53 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt > 1:4039332/1‑71 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt > 1:748230/1‑71 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGaaa > 1:2893918/1‑46 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGaaa > 1:2560824/1‑46 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:938310/67‑1 (MQ=255)
aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:2239805/67‑1 (MQ=255)
cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAg > 1:968424/1‑45 (MQ=255)
cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAg > 1:2128206/1‑45 (MQ=255)
cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:1517579/1‑71 (MQ=255)
cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:537974/1‑71 (MQ=255)
cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGCAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:3090922/1‑71 (MQ=255)
aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3244599/1‑47 (MQ=255)
aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:1564449/1‑47 (MQ=255)
aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa > 1:1970842/1‑46 (MQ=255)
aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:1117179/40‑1 (MQ=255)
aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:1845397/40‑1 (MQ=255)
aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:3464146/40‑1 (MQ=255)
aaaaaTTACACAAAATCATTAGCACTGCATCGAGACGGaa < 1:3354295/40‑1 (MQ=255)
aaTTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTAc > 1:965252/1‑71 (MQ=255)
aTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACg > 1:2084106/1‑71 (MQ=255)
ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:3911050/71‑1 (MQ=255)
ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:3723892/1‑71 (MQ=255)
ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:2673864/1‑71 (MQ=255)
ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:1530298/71‑1 (MQ=255)
ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:2834764/1‑71 (MQ=255)
tACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:2122799/1‑70 (MQ=255)
acacAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg < 1:2434753/61‑1 (MQ=255)
tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAccc > 1:442106/1‑56 (MQ=255)
gCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc < 1:632652/43‑1 (MQ=255)
cATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAg > 1:2312653/1‑70 (MQ=255)
|
AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463992‑4464123
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
