Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
480,905 |
C→T |
G908E (GGG→GAG) |
acrB ← |
multidrug efflux system protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 480,905 | 0 | C | T | 100.0%
| 167.8
/ NA
| 52 | G908E (GGG→GAG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (40/12); total (40/12) |
GTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAG > W3110S.gb/480850‑480972
|
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:1088484/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:3659218/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:3420965/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:3267027/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:820740/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:2289253/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:2172310/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:2021798/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:1870901/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:139490/1‑69 (MQ=255)
gTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGAc > 1:1395044/1‑69 (MQ=255)
gTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGgaga > 1:1846182/1‑70 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:2459771/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:3694406/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:4115757/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:1102290/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:2485314/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:1840382/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:2060969/1‑71 (MQ=255)
gCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGaa > 1:1970997/1‑71 (MQ=255)
ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAAt > 1:464214/1‑71 (MQ=255)
ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAAt > 1:1078406/1‑71 (MQ=255)
ccACGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAAt > 1:855210/1‑71 (MQ=255)
aCGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCg < 1:2351701/71‑1 (MQ=255)
aCGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCg < 1:2356581/71‑1 (MQ=255)
cGGAAGGTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCg < 1:331860/70‑1 (MQ=255)
gTGGCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGct < 1:682819/71‑1 (MQ=255)
gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct < 1:2640409/70‑1 (MQ=255)
gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct < 1:2168787/70‑1 (MQ=255)
gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct < 1:1222515/70‑1 (MQ=255)
gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct < 1:820848/70‑1 (MQ=255)
gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct < 1:19506/70‑1 (MQ=255)
gCAGCCAGCAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGctct < 1:1693871/70‑1 (MQ=255)
agcAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAg < 1:1236721/71‑1 (MQ=255)
agcAACGCACCGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAg < 1:3937031/71‑1 (MQ=255)
ccGATATCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:527776/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:804349/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:956342/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:4025191/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:3626084/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:2545043/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:2395450/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccgc > 1:1515798/1‑68 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccg > 1:3938339/1‑67 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgccg > 1:2021732/1‑67 (MQ=255)
ccGATAACCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGcgacgc > 1:2060837/1‑68 (MQ=255)
aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg > 1:3777097/1‑71 (MQ=255)
aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg > 1:3741713/1‑71 (MQ=255)
aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg > 1:4109802/1‑71 (MQ=255)
aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg > 1:3365355/1‑71 (MQ=255)
aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg > 1:3361499/1‑71 (MQ=255)
aCCTCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAg > 1:2701715/1‑71 (MQ=255)
|
GTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAG > W3110S.gb/480850‑480972
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
